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	|Institute-logo=Logo MPI-CBG.jpg		|Institute-logo=Logo MPI-CBG.jpg
	|description=Zeiss LSM 780 NLO		|description=Zeiss LSM 780 NLO
−	*Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.<br>	+	Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
−	*Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition	+	Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition
	|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS		|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS
	|image=MZ1 - Zeiss 2photon upright.jpg		|image=MZ1 - Zeiss 2photon upright.jpg
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	|ill8=Laser Multiphoton Chameleon 700-1064 nm		|ill8=Laser Multiphoton Chameleon 700-1064 nm
	|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br>		|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br>
−	'''2 photon detection:''' Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP	+	'''2 photon detection:''' 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction, 2 non-descanned PMTs (1 NDD moule) in transmitted direction
	|reflectors=Zeiss set 49: DAPI<br>		|reflectors=Zeiss set 49: DAPI<br>
	Zeiss set 38 Endow: GFP<br>		Zeiss set 38 Endow: GFP<br>

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Revision as of 19:12, 3 February 2016

Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS.

Directions

MPI-CBG LMF (MPI-CBG, 038C)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Zeiss - Axio Examiner.Z1, Upright stand, motorized XY stage, motorized Z-drive,
objectives	Zeiss EC Plan-Neofluar 10x 0.3 Zeiss Plan-Apochromat 10x 0.45 Zeiss Clr Plan-Apochromat 10x 0.5 Scale Zeiss N-Achroplan 20x 0.5 W Zeiss Plan-Apochromat 20x 0.8 Zeiss W Plan-Apochromat 20x 1.0 DIC Zeiss Clr Plan-Neofluar 20x 1.0 Korr Clarity Zeiss LD SC Plan-Apochromat 20x 1.0 Korr Scale Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC Zeiss C-Apochromat 40x 1.2 W
illumination	Fluorescence (Metal Halide, HXP, 120W) Transmitted Light (Halogen) Laser Diode 405 nm Laser Argon Multiline 458, 488, 514 nm Laser DPSS 561 nm Laser HeNe 594 nm Laser HeNe 633 nm Laser Multiphoton Chameleon 700-1064 nm
detection	Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting 2 photon detection: 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction, 2 non-descanned PMTs (1 NDD moule) in transmitted direction
reflectors	Zeiss set 49: DAPI Zeiss set 38 Endow: GFP Zeiss set 45: HQ Texas Red
features	simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software	Zen 2012 black
incubation	Not available
links	manual laser power values [ beam path] [ advanced system check]
inv.nr.	AIM System 2501000445 10.1.145.41 (IPv4)

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