MZ2 - Zeiss 2photon inverted

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{{Equipment
 
{{Equipment
 
|system-number=MPI-MZ2
 
|system-number=MPI-MZ2
|system-name=Zeiss 2-photon inverted
+
|system-name=MZ2 - Zeiss 2-photon inverted
 
|type=Microscope
 
|type=Microscope
 
|category=Multiphoton Laser Scanning
 
|category=Multiphoton Laser Scanning

Revision as of 16:11, 26 April 2010

  • Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser for multiphoton excitation.
  • Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 710 NLO
  • Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser for multiphoton excitation.
  • Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan..
MZ1-Zeiss 2photon upr.png


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 456)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axio Observer, Inverted stand, motorized XY stage, motorized Z-drive,
objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss C-Apochromat 40x 1.2 W
illumination
Fluorescence (Metal Halide, PRIOR, 200W)
Transmitted Light (Halogen)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
Laser Multiphoton Chameleon 700-1064 nm
detection [[detection::Single photon detection: 2 confocal PMTs, QUASAR detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting

2 photon detection: 2 non-descanned detectors (NDD) - NDD-dichromatic mirrors and emission filters]]

reflectors -
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software ZEN 2009
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. AIM-System 2501000455



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