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	|Facility-logo=MPI-CBG-Lmf-logo-big.png		|Facility-logo=MPI-CBG-Lmf-logo-big.png
	|Institute-logo=Logo MPI-CBG.jpg		|Institute-logo=Logo MPI-CBG.jpg
−	|description=Zeiss LSM 710 NLO<br>Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multi-photon excitation.	+	|description=Zeiss LSM 710 NLO<br>Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multi-photon excitation.<br>
−	<br>Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition	+	Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition
	|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan.		|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan.
	|image=MZ2 - Zeiss 2photon inverted.jpg		|image=MZ2 - Zeiss 2photon inverted.jpg

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Revision as of 19:20, 3 February 2016

Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 710 NLO<br />Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multi-photon excitation.<br /> Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan..

Directions

MPI-CBG LMF (MPI-CBG, 456A)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Zeiss - Axio Observer, Inverted stand, motorized XY stage, motorized Z-drive,
objectives	Zeiss Plan-Apochromat 10x 0.45 Zeiss C-Apochromat 40x 1.2 W Zeiss Plan-Apochromat 20x 0.8 Zeiss Plan-Apochromat 63x 1.4 Oil Zeiss LD C-Apochromat 40x 1.1 W Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
illumination	Fluorescence (Metal Halide, PRIOR, 200W) Transmitted Light (Halogen) Laser Diode 405 nm Laser Argon Multiline 458, 477, 488, 514 nm Laser DPSS 561 nm Laser HeNe 594 nm Laser HeNe 633 nm Laser Multiphoton Chameleon 700-1064 nm
detection	[[detection::Single photon detection: 2 confocal PMTs, QUASAR detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting 2 photon detection: 2 non-descanned detectors (NDD) - NDD-dichromatic mirrors and emission filters]]
reflectors	-
features	simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software	ZEN 2010
incubation	Not available
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.	AIM-System 2501000455 10.1.30.42

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