- Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multi-photon excitation.
- Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 710 NLO
- Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multi-photon excitation.
- Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.
Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan..
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope
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Zeiss - Axio Observer, Inverted stand, motorized XY stage, motorized Z-drive,
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objectives
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- Zeiss PlanApo 10X/0.45 W
- Zeiss C-Apochromat 40x 1.2 W
- Zeiss PlanApo 20X/0.8
- Zeiss PlanApo 63X/1.4 Oil
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illumination
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- Fluorescence (Metal Halide, PRIOR, 200W)
- Transmitted Light (Halogen)
- Laser Diode 405 nm
- Laser Argon Multiline 458, 477, 488, 514 nm
- Laser DPSS 561 nm
- Laser HeNe 594 nm
- Laser HeNe 633 nm
- Laser Multiphoton Chameleon 700-1064 nm
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detection
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[[detection::Single photon detection: 2 confocal PMTs, QUASAR detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting
2 photon detection: 2 non-descanned detectors (NDD) - NDD-dichromatic mirrors and emission filters]]
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reflectors
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features
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simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
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software
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ZEN 2010
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incubation
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Not available
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links
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- [ manual]
- [ laser power values]
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- [ beam path]
- [ advanced system check]
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inv.nr.
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AIM-System 2501000455 10.1.30.42
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