PeterEvennettCourse04-2010

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==Peter Evennett's Course Oct 09 ==
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==Peter Evennett's Course April 2010 ==
  
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=== Friday 16th Oct ===
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=== Setup -Fri 16th Apr ===
 
Setup - all lmf staff move stuff to galleria
 
Setup - all lmf staff move stuff to galleria
 
Equipment needed
 
Equipment needed
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=== DAY ONE (October 19th)===
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=== DAY ONE (19th Apr)===
  
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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|}
 
|}
  
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=== DAY TWO (October 20th)===
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=== DAY TWO (Apr 20th)===
  
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 
|-
 
|-
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| ||||||||
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| 9:00||9:30||align="center"|Recap ||align="center" | day 1  ||align="center" |LMF TEAM
 
|-
 
|-
 
| 9:30||10:45||align="center"|SESSION 1 <br>Theory & Practice||align="center" |  Resolution<br>Diffraction  ||align="center" |PETER EVENNETT <br>LMF TEAM
 
| 9:30||10:45||align="center"|SESSION 1 <br>Theory & Practice||align="center" |  Resolution<br>Diffraction  ||align="center" |PETER EVENNETT <br>LMF TEAM
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|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''    '''
 
|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''    '''
 
|-
 
|-
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| 16:15|18:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
+
| 16:15||18:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
 
|-
 
|-
 
| ||||||||
 
| ||||||||
 
|}
 
|}
  
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=== DAY THREE (October 21th)===
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=== DAY THREE (Apr 21th)===
  
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 
|-
 
|-
−
| ||||||||
+
| 9:00||9:30||align="center"|Recap ||align="center" | day 2  ||align="center" |LMF TEAM
 
|-
 
|-
 
| 9:30||11:30||align="center"|SESSION 1<br>Theory & Practice||align="center" | Objective reading<br> Objective cleaning||align="center" |PETER EVENNETT <br>LMF TEAM
 
| 9:30||11:30||align="center"|SESSION 1<br>Theory & Practice||align="center" | Objective reading<br> Objective cleaning||align="center" |PETER EVENNETT <br>LMF TEAM
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|}
 
|}
  
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=== DAY FOUR (October 22th)===
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=== DAY FOUR (Apr 22th)===
  
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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| align="center" style="background:#f0f0f0;"|'''DEMO materials'''
 
| align="center" style="background:#f0f0f0;"|'''DEMO materials'''
 
|-
 
|-
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| ||||||||||||
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| 9:00||9:30||align="center"|Recap ||align="center" | day 3  ||align="center" |LMF TEAM ||||
 
|-
 
|-
 
| 9:30||10:20||align="center"|SESSION 1<br>Theory||align="center" | Question session <br> Fundamentals concepts of fluorescence ||align="center" |LMF TEAM<br>Dan||align="center" |- ||align="center" |-  
 
| 9:30||10:20||align="center"|SESSION 1<br>Theory||align="center" | Question session <br> Fundamentals concepts of fluorescence ||align="center" |LMF TEAM<br>Dan||align="center" |- ||align="center" |-  
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| ||||||||||||
 
|-
 
|-
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| 10:55||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Humberto||align="center" |• Mercury lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions
+
| 10:55||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Dan? Sike? Jan?||align="center" |• Mercury/Xenon/MetalHalide lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions
 
|-
 
|-
 
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| 14:15||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD ||align="center" |LMF TEAM<br> Britta||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • Cameras
+
| 14:15||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD and Quantitative Imaging ||align="center" |LMF TEAM<br> Britta/Dan||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • RGB Slider Spot Camera setup
 
|-
 
|-
 
| style="background:DarkKhaki;"|'''15:45'''
 
| style="background:DarkKhaki;"|'''15:45'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''
 
|-
 
|-
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| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM<br>Jan / Peter / Dan / Britta (*)||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes (4) <br>• Sample slides
+
| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM<br>Jan / Peter / Dan / Britta / Silke (*)||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM <br>• Fluorescence Sample slides (Kidney / Cells
 
|}
 
|}
 
''
 
''
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| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 +
|-
 +
| 9:00||9:30||align="center"|Recap ||align="center" |  day 4  ||align="center" |LMF TEAM
 
|-
 
|-
 
| 9:30||10:20||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
 
| 9:30||10:20||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
 
|-
 
|-
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|-
 
| style="background:DarkKhaki;"|'''10:20'''
 
| style="background:DarkKhaki;"|'''10:20'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
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|-
 
|-
 
| 10:35||13:00||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
 
| 10:35||13:00||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
 
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|-
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|-
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| 14:00||15:15||align="center"|SESSION 2<br>Theory & Demo|| align="center"|Basics of Quantitative Image Analysis <br> (Image Processing) ||align="center" |LMF TEAM<br>Dan
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| 14:00||15:15||align="center"|SESSION 2<br>Small Groups Discussion|| align="center"|What techniques might be good for me<br> (Own Projects) ||align="center" |LMF TEAM<br>Dan/Jan/Silke/Britta/Pete?
 
|-
 
|-
 
| style="background:DarkKhaki;"|'''15:15'''
 
| style="background:DarkKhaki;"|'''15:15'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
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| ||||||||
 
 
|-
 
|-
 
| 15:30||16:30||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM
 
| 15:30||16:30||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM
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=== OFFICE DUTIES===
 
=== OFFICE DUTIES===
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==== - LMF Members available for users (19th Oct to 23rd Oct) - ====
+
==== - LMF Members available for users (19th Apr to 23rd Apr) - ====
  
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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|-
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| 9:30||12:30||align="center"| - ||align="center" | Britta ||align="center" |Peter ||align="center" |Peter ||align="center" |Silke
+
| 9:30||12:30||align="center"| - ||align="center" | Silke ||align="center" |Peter ||align="center" |Peter ||align="center" |Britta
 
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|-
 
| style="background:DarkKhaki;"|'''12:30'''
 
| style="background:DarkKhaki;"|'''12:30'''
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| 13:30||18:00||align="center"|Britta|| align="center"|Dan ||align="center" |Silke ||align="center" |(*)Silke <br> Humberto||align="center" |-
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| 13:30||18:00||align="center"|Britta|| align="center"|Dan ||align="center" |Dan ||align="center" |Silke||align="center" |-
 
|}
 
|}
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(*) Silke and Humberto will be cleaning and reorganizing the Galeria
 

Latest revision as of 11:55, 13 April 2010

Contents

[edit] Peter Evennett's Course April 2010

[edit] Setup -Fri 16th Apr

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


[edit] DAY ONE (19th Apr)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:05 INTRODUCTION COURSE PRESENTATION
GROUP PRESENTATION		 LMF TEAM
11:05 11:20 BREAK BREAK BREAK
11:20 13:00 SESSION 1
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects of microscopy
Introduction to lenses
Lens aberrations
Magnification
Numerical aperture
Microscope illumination
Conjugate planes
Koehler illumination		 PETER EVENNETT
LMF TEAM
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:30 SESSION 2
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY
Demo		 PETER EVENNETT
LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 18:00 SESSION 3
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY PETER EVENNETT
LMF TEAM

[edit] DAY TWO (Apr 20th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap day 1 LMF TEAM
9:30 10:45 SESSION 1
Theory & Practice		 Resolution
Diffraction 		PETER EVENNETT
LMF TEAM
10:45 11:00 BREAK COFFEE BREAK
11:00 13:00 DEMONSTRATION Abbe´s diffraction experiments
 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 16:00 SESSION 2
Theory & Practice		 Bright Field
Dark Field
Phase Contrast microscopy 		PETER EVENNETT
LMF TEAM
16:00 16:15 BREAK COFFEE BREAK
16:15 18:00 SESSION 2
Theory & Practice		 Bright Field
Dark Field
Phase Contrast microscopy 		PETER EVENNETT
LMF TEAM

[edit] DAY THREE (Apr 21th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap day 2 LMF TEAM
9:30 11:30 SESSION 1
Theory & Practice		 Objective reading
Objective cleaning		 PETER EVENNETT
LMF TEAM
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 SESSION 2
Theory & Practice		 Polarized light microscopy
Differential Interference Contrast (DIC)		 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 3
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM
15:15 15:30 BREAK BREAK BREAK
15:30 18:00 SESSION 4
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM

[edit] DAY FOUR (Apr 22th)

FROM TO ACTIVITY TOPICS Responsible person(s) DEMO Activities DEMO materials
9:00 9:30 Recap day 3 LMF TEAM
9:30 10:20 SESSION 1
Theory		 Question session
Fundamentals concepts of fluorescence 		LMF TEAM
Dan		 - -
10:20 10:55 BREAK BREAK BREAK BREAK BREAK
10:55 13:15 SESSION 2
Theory & Practice		 Fluorescence Microscopy LMF TEAM
Dan? Sike? Jan?		 • Mercury/Xenon/MetalHalide lamp
• Lamp houses
• Spectra
• Filters
• Filter cubes
• Fluorescent-labeled samples
• Filter spectral charts of each microscope (laminated)		 • Spare lamp houses
• Lamps
• Ocean optics equipment
• Different filters
• Four (4) different Filter-sets (cubes), with the appropriate descriptions
13:15 14:15 BREAK LUNCH LUNCH LUNCH LUNCH
14:15 15:45 SESSION 3
Theory & Practice		 Imaging with CCD and Quantitative Imaging LMF TEAM
Britta/Dan		 • CCD
• Cameras 		• CCD chip
• RGB Slider Spot Camera setup
15:45 16:00 BREAK BREAK
16:00 18:00 SESSION 4
Practice		 Imaging with CCD cameras using LMF systems LMF TEAM
Jan / Peter / Dan / Britta / Silke (*)		 • Displaying
• Pixel size
• Look up table
• Binning
• Exposure time
• Saturation
• Signal to noise
• ROI
• Bit depth
• Auto map
• Auto contrast 		• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM
• Fluorescence Sample slides (Kidney / Cells

[edit] DAY FIVE (October 23th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap day 4 LMF TEAM
9:30 10:20 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
10:20 10:35 BREAK BREAK BREAK
10:35 13:00 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 2
Small Groups Discussion		 What techniques might be good for me
(Own Projects) 		LMF TEAM
Dan/Jan/Silke/Britta/Pete?
15:15 15:30 BREAK BREAK BREAK
15:30 16:30 SESSION 3 Course evaluation LMF TEAM

[edit] OFFICE DUTIES

[edit] - LMF Members available for users (19th Apr to 23rd Apr) -

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
9:30 12:30 - Silke Peter Peter Britta
12:30 13:30 L U N C H
13:30 18:00 Britta Dan Dan Silke -
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