PeterEvennettCourse04-2010

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(create page using content from last PE course)
 
(changed dates and revides content for friday and thurs, quantitative imaging moved after ccd talk)
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==Peter Evennett's Course Oct 09 ==
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==Peter Evennett's Course April 2010 ==
  
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=== Friday 16th Oct ===
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=== Setup -Fri 16th Apr ===
 
Setup - all lmf staff move stuff to galleria
 
Setup - all lmf staff move stuff to galleria
 
Equipment needed
 
Equipment needed
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=== DAY ONE (October 19th)===
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=== DAY ONE (19th Apr)===
  
 
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=== DAY TWO (October 20th)===
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=== DAY TWO (Apr 20th)===
  
 
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=== DAY THREE (October 21th)===
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=== DAY THREE (Apr 21th)===
  
 
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=== DAY FOUR (October 22th)===
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=== DAY FOUR (Apr 22th)===
  
 
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| 10:55||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Humberto||align="center" |• Mercury lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions
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| 10:55||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Dan? Sike? Jan?||align="center" |• Mercury/Xenon/MetalHalide lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions
 
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| 14:15||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD ||align="center" |LMF TEAM<br> Britta||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • Cameras
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| 14:15||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD and Quantitative Imaging ||align="center" |LMF TEAM<br> Britta/Dan||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • RGB Slider Spot Camera setup
 
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|align="center" style="background:DarkKhaki;"|'''BREAK'''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''
 
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| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM<br>Jan / Peter / Dan / Britta (*)||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes (4) <br>• Sample slides
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| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM<br>Jan / Peter / Dan / Britta / Silke (*)||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM <br>• Fluorescence Sample slides (Kidney / Cells
 
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| 14:00||15:15||align="center"|SESSION 2<br>Theory & Demo|| align="center"|Basics of Quantitative Image Analysis <br> (Image Processing) ||align="center" |LMF TEAM<br>Dan
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| 14:00||15:15||align="center"|SESSION 2<br>Small Groups Discussion|| align="center"|What techniques might be good for me<br> (Owen Projects) ||align="center" |LMF TEAM<br>Dan/Jan/Silke/Britta/Pete?
 
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| style="background:DarkKhaki;"|'''15:15'''
 
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=== OFFICE DUTIES===
 
=== OFFICE DUTIES===
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==== - LMF Members available for users (19th Oct to 23rd Oct) - ====
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==== - LMF Members available for users (19th Apr to 23rd Apr) - ====
  
 
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| 13:30||18:00||align="center"|Britta|| align="center"|Dan ||align="center" |Silke ||align="center" |(*)Silke <br> Humberto||align="center" |-
 
| 13:30||18:00||align="center"|Britta|| align="center"|Dan ||align="center" |Silke ||align="center" |(*)Silke <br> Humberto||align="center" |-
 
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(*) Silke and Humberto will be cleaning and reorganizing the Galeria
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(*) ???? will be cleaning and reorganizing the Galeria

Revision as of 11:31, 13 April 2010

Contents

Peter Evennett's Course April 2010

Setup -Fri 16th Apr

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (19th Apr)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:05 INTRODUCTION COURSE PRESENTATION
GROUP PRESENTATION		 LMF TEAM
11:05 11:20 BREAK BREAK BREAK
11:20 13:00 SESSION 1
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects of microscopy
Introduction to lenses
Lens aberrations
Magnification
Numerical aperture
Microscope illumination
Conjugate planes
Koehler illumination		 PETER EVENNETT
LMF TEAM
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:30 SESSION 2
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY
Demo		 PETER EVENNETT
LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 18:00 SESSION 3
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY PETER EVENNETT
LMF TEAM

DAY TWO (Apr 20th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:45 SESSION 1
Theory & Practice		 Resolution
Diffraction 		PETER EVENNETT
LMF TEAM
10:45 11:00 BREAK COFFEE BREAK
11:00 13:00 DEMONSTRATION Abbe´s diffraction experiments
 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 16:00 SESSION 2
Theory & Practice		 Bright Field
Dark Field
Phase Contrast microscopy 		PETER EVENNETT
LMF TEAM
16:00 16:15 BREAK COFFEE BREAK
18:00 SESSION 2
Theory & Practice		 Bright Field
Dark Field
Phase Contrast microscopy 		PETER EVENNETT
LMF TEAM

DAY THREE (Apr 21th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:30 SESSION 1
Theory & Practice		 Objective reading
Objective cleaning		 PETER EVENNETT
LMF TEAM
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 SESSION 2
Theory & Practice		 Polarized light microscopy
Differential Interference Contrast (DIC)		 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 3
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM
15:15 15:30 BREAK BREAK BREAK
15:30 18:00 SESSION 4
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM

DAY FOUR (Apr 22th)

FROM TO ACTIVITY TOPICS Responsible person(s) DEMO Activities DEMO materials
9:30 10:20 SESSION 1
Theory		 Question session
Fundamentals concepts of fluorescence 		LMF TEAM
Dan		 - -
10:20 10:55 BREAK BREAK BREAK BREAK BREAK
10:55 13:15 SESSION 2
Theory & Practice		 Fluorescence Microscopy LMF TEAM
Dan? Sike? Jan?		 • Mercury/Xenon/MetalHalide lamp
• Lamp houses
• Spectra
• Filters
• Filter cubes
• Fluorescent-labeled samples
• Filter spectral charts of each microscope (laminated)		 • Spare lamp houses
• Lamps
• Ocean optics equipment
• Different filters
• Four (4) different Filter-sets (cubes), with the appropriate descriptions
13:15 14:15 BREAK LUNCH LUNCH LUNCH LUNCH
14:15 15:45 SESSION 3
Theory & Practice		 Imaging with CCD and Quantitative Imaging LMF TEAM
Britta/Dan		 • CCD
• Cameras 		• CCD chip
• RGB Slider Spot Camera setup
15:45 16:00 BREAK BREAK
16:00 18:00 SESSION 4
Practice		 Imaging with CCD cameras using LMF systems LMF TEAM
Jan / Peter / Dan / Britta / Silke (*)		 • Displaying
• Pixel size
• Look up table
• Binning
• Exposure time
• Saturation
• Signal to noise
• ROI
• Bit depth
• Auto map
• Auto contrast 		• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM
• Fluorescence Sample slides (Kidney / Cells

DAY FIVE (October 23th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:20 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
10:20 10:35 BREAK BREAK BREAK
10:35 13:00 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 2
Small Groups Discussion		 What techniques might be good for me
(Owen Projects) 		LMF TEAM
Dan/Jan/Silke/Britta/Pete?
15:15 15:30 BREAK BREAK BREAK
15:30 16:30 SESSION 3 Course evaluation LMF TEAM

OFFICE DUTIES

- LMF Members available for users (19th Apr to 23rd Apr) -

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
9:30 12:30 - Britta Peter Peter Silke
12:30 13:30 L U N C H
13:30 18:00 Britta Dan Silke (*)Silke
Humberto		 -

(*) ???? will be cleaning and reorganizing the Galeria

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