PeterEvennettCourse04-2010
From BioDIP
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| align="center" style="background:#f0f0f0;"|'''TOPICS''' | | align="center" style="background:#f0f0f0;"|'''TOPICS''' | ||
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)''' | | align="center" style="background:#f0f0f0;"|'''Responsible person(s)''' | ||
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| + | | 9:00||9:30||align="center"|Recap ||align="center" | day 4 ||align="center" |LMF TEAM | ||
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| 9:30||10:20||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan | | 9:30||10:20||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan | ||
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK''' | |align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK''' | ||
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| 10:35||13:00||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan | | 10:35||13:00||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan | ||
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|''' ''' | |align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|''' ''' | ||
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| − | | 14:00||15:15||align="center"|SESSION 2<br>Small Groups Discussion|| align="center"|What techniques might be good for me<br> ( | + | | 14:00||15:15||align="center"|SESSION 2<br>Small Groups Discussion|| align="center"|What techniques might be good for me<br> (Own Projects) ||align="center" |LMF TEAM<br>Dan/Jan/Silke/Britta/Pete? |
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| style="background:DarkKhaki;"|'''15:15''' | | style="background:DarkKhaki;"|'''15:15''' | ||
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|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK''' | |align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK''' | ||
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| 15:30||16:30||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM | | 15:30||16:30||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM | ||
Latest revision as of 11:55, 13 April 2010
Contents |
[edit] Peter Evennett's Course April 2010
[edit] Setup -Fri 16th Apr
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
[edit] DAY ONE (19th Apr)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:30 | 11:05 | INTRODUCTION | COURSE PRESENTATION GROUP PRESENTATION |
LMF TEAM |
| 11:05 | 11:20 | BREAK | BREAK | BREAK |
| 11:20 | 13:00 | SESSION 1 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Historical aspects of microscopy Introduction to lenses Lens aberrations Magnification Numerical aperture Microscope illumination Conjugate planes Koehler illumination |
PETER EVENNETT LMF TEAM |
| 13:00 | 14:00 | LUNCH | LUNCH | LUNCH |
| 14:00 | 15:30 | SESSION 2 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Demo |
PETER EVENNETT LMF TEAM |
| 15:30 | 15:45 | BREAK | BREAK | BREAK |
| 15:45 | 18:00 | SESSION 3 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY | PETER EVENNETT LMF TEAM |
[edit] DAY TWO (Apr 20th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:30 | Recap | day 1 | LMF TEAM |
| 9:30 | 10:45 | SESSION 1 Theory & Practice |
Resolution Diffraction |
PETER EVENNETT LMF TEAM |
| 10:45 | 11:00 | BREAK | COFFEE BREAK | |
| 11:00 | 13:00 | DEMONSTRATION | Abbe´s diffraction experiments |
PETER EVENNETT LMF TEAM |
| 13:00 | 14:00 | BREAK | LUNCH | |
| 14:00 | 16:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM |
| 16:00 | 16:15 | BREAK | COFFEE BREAK | |
| 16:15 | 18:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM |
[edit] DAY THREE (Apr 21th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:30 | Recap | day 2 | LMF TEAM |
| 9:30 | 11:30 | SESSION 1 Theory & Practice |
Objective reading Objective cleaning |
PETER EVENNETT LMF TEAM |
| 11:30 | 11:45 | BREAK | BREAK | BREAK |
| 11:45 | 13:00 | SESSION 2 Theory & Practice |
Polarized light microscopy Differential Interference Contrast (DIC) |
PETER EVENNETT LMF TEAM |
| 13:00 | 14:00 | BREAK | LUNCH | |
| 14:00 | 15:15 | SESSION 3 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
| 15:15 | 15:30 | BREAK | BREAK | BREAK |
| 15:30 | 18:00 | SESSION 4 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
[edit] DAY FOUR (Apr 22th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | DEMO Activities | DEMO materials |
| 9:00 | 9:30 | Recap | day 3 | LMF TEAM | ||
| 9:30 | 10:20 | SESSION 1 Theory |
Question session Fundamentals concepts of fluorescence |
LMF TEAM Dan |
- | - |
| 10:20 | 10:55 | BREAK | BREAK | BREAK | BREAK | BREAK |
| 10:55 | 13:15 | SESSION 2 Theory & Practice |
Fluorescence Microscopy | LMF TEAM Dan? Sike? Jan? |
• Mercury/Xenon/MetalHalide lamp • Lamp houses • Spectra • Filters • Filter cubes • Fluorescent-labeled samples • Filter spectral charts of each microscope (laminated) |
• Spare lamp houses • Lamps • Ocean optics equipment • Different filters • Four (4) different Filter-sets (cubes), with the appropriate descriptions |
| 13:15 | 14:15 | BREAK | LUNCH | LUNCH | LUNCH | LUNCH |
| 14:15 | 15:45 | SESSION 3 Theory & Practice |
Imaging with CCD and Quantitative Imaging | LMF TEAM Britta/Dan |
• CCD • Cameras |
• CCD chip • RGB Slider Spot Camera setup |
| 15:45 | 16:00 | BREAK | BREAK | |||
| 16:00 | 18:00 | SESSION 4 Practice |
Imaging with CCD cameras using LMF systems | LMF TEAM Jan / Peter / Dan / Britta / Silke (*) |
• Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast |
• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM • Fluorescence Sample slides (Kidney / Cells |
[edit] DAY FIVE (October 23th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:30 | Recap | day 4 | LMF TEAM |
| 9:30 | 10:20 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
| 10:20 | 10:35 | BREAK | BREAK | BREAK |
| 10:35 | 13:00 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
| 13:00 | 14:00 | BREAK | LUNCH | |
| 14:00 | 15:15 | SESSION 2 Small Groups Discussion |
What techniques might be good for me (Own Projects) |
LMF TEAM Dan/Jan/Silke/Britta/Pete? |
| 15:15 | 15:30 | BREAK | BREAK | BREAK |
| 15:30 | 16:30 | SESSION 3 | Course evaluation | LMF TEAM |
[edit] OFFICE DUTIES
[edit] - LMF Members available for users (19th Apr to 23rd Apr) -
| FROM | TO | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
| 9:30 | 12:30 | - | Silke | Peter | Peter | Britta |
| 12:30 | 13:30 | L | U | N | C | H |
| 13:30 | 18:00 | Britta | Dan | Dan | Silke | - |
