Peter Evennett's Course Oct 09
From BioDIP
(Difference between revisions)
(page or schedule of peter evennett course oct 09, stuff needed, and responsibilities) |
(added schedule table test - exported word doc from open office to media wiki markup) |
||
Line 11: | Line 11: | ||
+ | |||
+ | <center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center> | ||
+ | |||
+ | <center>'''Week 2 '''</center> | ||
+ | |||
+ | |||
+ | |||
+ | {| class="prettytable" | ||
+ | | <center>'''FROM'''</center> | ||
+ | | <center>'''TO'''</center> | ||
+ | | <center>'''ACTIVITY'''</center> | ||
+ | | <center>'''MONDAY (Dec 1)'''</center> | ||
+ | | <center>'''Responsible person(s)'''</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''9:00'''</center> | ||
+ | | <center>'''10:30'''</center> | ||
+ | | <center>'''INTRODUCTION'''</center> | ||
+ | |||
+ | |||
+ | |||
+ | | <center>Group presentation</center> | ||
+ | |||
+ | <center>Wishes for the course</center> | ||
+ | |||
+ | <center>The ideal microscope</center> | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''DEMO'''</center> | ||
+ | | <center>Optical bench</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''10:30'''</center> | ||
+ | | <center>'''11:00'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>Break</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''11:00'''</center> | ||
+ | |||
+ | |||
+ | |||
+ | | <center>'''12:30'''</center> | ||
+ | | <center>'''LECTURE 1'''</center> | ||
+ | | <center>Basics in Optics</center> | ||
+ | |||
+ | <center>Characteristics of light</center> | ||
+ | |||
+ | <center>Light sources</center> | ||
+ | |||
+ | <center>Reflection, Absorption, Refraction</center> | ||
+ | |||
+ | <center>Diffraction (Demo)</center> | ||
+ | | <center>Humberto</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 1'''</center> | ||
+ | |||
+ | <center>'''(Group Rotation)'''</center> | ||
+ | | # <nowiki>1. [Capillary + oil] + </nowiki>Coin in glass | ||
+ | |||
+ | 2. Laser refraction + reflection | ||
+ | |||
+ | # 3. Different objectives – Demo TIRF Objective (immersion solution) | ||
+ | |||
+ | |||
+ | | # 1. Peter | ||
+ | |||
+ | 2. Humberto | ||
+ | |||
+ | # 3. Britta | ||
+ | |||
+ | |||
+ | |||
+ | |- | ||
+ | | <center>'''12:30'''</center> | ||
+ | | <center>'''13:30'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>LUNCH</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''13:30'''</center> | ||
+ | | <center>'''15:30'''</center> | ||
+ | | <center>'''LECTURE 2'''</center> | ||
+ | | <center>Introduction to lenses</center> | ||
+ | |||
+ | <center>Lens aberrations</center> | ||
+ | |||
+ | <center>Understanding ray optics</center> | ||
+ | | <center>Humberto</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 2'''</center> | ||
+ | | <center>Ray optics</center> | ||
+ | |||
+ | <center>Drawing ray graphics/diagrams</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 3'''</center> | ||
+ | | # 1. Finding focal point of lenses + infinity + Color fringes | ||
+ | # 2. Virtual & real images. 25cm distance | ||
+ | # 3. Image of the arrow in the microscope (upright – inverted) | ||
+ | |||
+ | |||
+ | | # 1. Jan | ||
+ | # 2. Humberto | ||
+ | # 3. Pete + Britta | ||
+ | |||
+ | |||
+ | |||
+ | |- | ||
+ | | <center>'''15:30'''</center> | ||
+ | | <center>'''15:50'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>Break</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''15:50'''</center> | ||
+ | | <center>'''17:00'''</center> | ||
+ | | <center>'''LECTURE 3'''</center> | ||
+ | | <center>Components of the microscope</center> | ||
+ | |||
+ | <center>Focusing, Field of view, Depth of field, depth of focus</center> | ||
+ | |||
+ | <center>Planes (filament DEMO)</center> | ||
+ | |||
+ | <center>Conjugated planes</center> | ||
+ | |||
+ | <center>Koehler illumination</center> | ||
+ | | <center>Humberto</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''17:00'''</center> | ||
+ | | <center>'''18:30'''</center> | ||
+ | | <center>'''ACTIVITY 4'''</center> | ||
+ | |||
+ | <center>'''Auditorium + Room 036'''</center> | ||
+ | | <center>Glass cube demo and practice - Koehler alignment + Document</center> | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | <center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center> | ||
+ | |||
+ | <center>'''Week 2 '''</center> | ||
+ | |||
+ | |||
+ | {| class="prettytable" | ||
+ | | <center>'''FROM'''</center> | ||
+ | | <center>'''TO'''</center> | ||
+ | | <center>'''ACTIVITY'''</center> | ||
+ | | <center>'''TUESDAY (Dec 2)'''</center> | ||
+ | | <center>'''Responsible person(s)'''</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''9:00'''</center> | ||
+ | | '''10:00''' | ||
+ | | <center>'''INTRODUCTION'''</center> | ||
+ | |||
+ | |||
+ | |||
+ | | <center>Koehler exercise</center> | ||
+ | |||
+ | <center>Questions</center> | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''10:00'''</center> | ||
+ | | <center>'''11:00'''</center> | ||
+ | | <center>'''LECTURE 4'''</center> | ||
+ | | <center>Diffraction </center> | ||
+ | |||
+ | Numerical aperture | ||
+ | | <center>Humberto</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 5'''</center> | ||
+ | | # Diffraction grids/gratings | ||
+ | |||
+ | |||
+ | | # | ||
+ | |||
+ | |||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 6'''</center> | ||
+ | | # Airy disk on the microscope on mirror | ||
+ | |||
+ | |||
+ | | # LMF TEAM | ||
+ | |||
+ | |||
+ | |||
+ | |- | ||
+ | | <center>'''11:00'''</center> | ||
+ | | <center>'''11:15'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>Break</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | '''11:15''' | ||
+ | | <center>'''11:30'''</center> | ||
+ | | <center>'''LECTURE 5'''</center> | ||
+ | | <center>Apertures (Objective and Condenser) - Cube DEMO</center> | ||
+ | | <center>Humberto</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''11:30'''</center> | ||
+ | | <center>'''13:00'''</center> | ||
+ | | <center>'''ACTIVITY 7'''</center> | ||
+ | |||
+ | |||
+ | |||
+ | | # Objective cleaning + NA reading + Cover slip thickness measurement | ||
+ | # Diffraction exercise on microscope | ||
+ | # Fluorescent beads with variable NA exercise | ||
+ | |||
+ | |||
+ | | # Pete | ||
+ | # Dan | ||
+ | # Britta | ||
+ | |||
+ | |||
+ | |||
+ | |- | ||
+ | | <center>'''13:00'''</center> | ||
+ | | <center>'''14:00'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>LUNCH</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''14:00'''</center> | ||
+ | | <center>'''15:45'''</center> | ||
+ | | <center>'''LECTURE 6'''</center> | ||
+ | | * <center><nowiki>Contrasting techniques [Dark field - Phase contrast]</nowiki></center> | ||
+ | |||
+ | |||
+ | | Humberto | ||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 8'''</center> | ||
+ | | Dark field | ||
+ | |||
+ | Phase contrast | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''15:45'''</center> | ||
+ | | <center>'''16:00'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>Break</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''16:00'''</center> | ||
+ | | <center>'''18:00'''</center> | ||
+ | | <center>'''LECTURE 7'''</center> | ||
+ | | * <center><nowiki>Contrasting techniques [Polarization – DIC]</nowiki></center> | ||
+ | |||
+ | |||
+ | | Humberto | ||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 9'''</center> | ||
+ | | Polarization – Polarization foils – Crystals | ||
+ | |||
+ | DIC | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | <center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center> | ||
+ | |||
+ | <center>'''Week 2 '''</center> | ||
+ | |||
+ | |||
+ | |||
+ | {| class="prettytable" | ||
+ | | <center>'''FROM'''</center> | ||
+ | | <center>'''TO'''</center> | ||
+ | | <center>'''ACTIVITY'''</center> | ||
+ | | <center>'''WEDNESDAY (Dec 3)'''</center> | ||
+ | | <center>'''Responsible person(s)'''</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''9:00'''</center> | ||
+ | | '''10:00''' | ||
+ | | <center>'''INTRODUCTION'''</center> | ||
+ | | <center>Questions</center> | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''10:00'''</center> | ||
+ | | <center>'''11:25'''</center> | ||
+ | | <center>'''LECTURE 8'''</center> | ||
+ | |||
+ | <center>'''(Interactive session)'''</center> | ||
+ | | <center>Objectives</center> | ||
+ | |||
+ | <center>Lens aberrations</center> | ||
+ | |||
+ | <center>Infinity optics </center> | ||
+ | | <center>Humberto</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''11:25'''</center> | ||
+ | | <center>'''11:15'''</center> | ||
+ | | '''ACTIVITY 10''' | ||
+ | | <center>1. Lens Aberrations</center> | ||
+ | |||
+ | <center>2. Objective reading</center> | ||
+ | |||
+ | <center>3. Infinity optics on optical bench</center> | ||
+ | | # 1. Humberto | ||
+ | |||
+ | 2. Britta | ||
+ | |||
+ | 3. Jan | ||
+ | |||
+ | |- | ||
+ | | <center>'''11:15'''</center> | ||
+ | | <center>'''11:30'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>Break</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''11:30'''</center> | ||
+ | | <center>'''13:00'''</center> | ||
+ | | <center>'''LECTURE 9'''</center> | ||
+ | | <center>Basics of fluorescence</center> | ||
+ | |||
+ | <center>Fluorochromes</center> | ||
+ | |||
+ | <center>The fluorescence microscope</center> | ||
+ | |||
+ | <center>Filters</center> | ||
+ | | <center>Humberto</center> | ||
+ | |||
+ | <center>Dan</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''13:00'''</center> | ||
+ | | <center>'''14:00'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>LUNCH</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''14:00'''</center> | ||
+ | | <center>'''15:00'''</center> | ||
+ | | <center>'''LECTURE 11'''</center> | ||
+ | | <center>CCD - DEMO</center> | ||
+ | | Britta | ||
+ | |||
+ | |- | ||
+ | | <center>'''15:00'''</center> | ||
+ | | <center>'''15:15'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>Break</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''15:15'''</center> | ||
+ | | <center>'''16:15'''</center> | ||
+ | | <center>'''LECTURE 12'''</center> | ||
+ | | <center>Digital imaging (live cell imaging – optional)</center> | ||
+ | | Dan | ||
+ | |||
+ | |- | ||
+ | | <center>'''16:15'''</center> | ||
+ | | <center>'''16:45'''</center> | ||
+ | | <center>'''ACTIVITY 11'''</center> | ||
+ | | Mercury lamp exchange and centering | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''16:45'''</center> | ||
+ | | <center>'''18:30'''</center> | ||
+ | | <center>'''ACTIVITY 12'''</center> | ||
+ | | Wide fields systems + CCD | ||
+ | |||
+ | Fluorescence | ||
+ | |||
+ | |||
+ | |||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | <center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center> | ||
+ | |||
+ | <center>'''Week 2 '''</center> | ||
+ | |||
+ | |||
+ | {| class="prettytable" | ||
+ | | <center>'''FROM'''</center> | ||
+ | | <center>'''TO'''</center> | ||
+ | | <center>'''ACTIVITY'''</center> | ||
+ | | <center>'''THURSDAY (Dec 4 )'''</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''9:00'''</center> | ||
+ | | '''9:45''' | ||
+ | | <center>'''INTRODUCTION'''</center> | ||
+ | |||
+ | |||
+ | |||
+ | | <center>Questions</center> | ||
+ | |||
+ | <center>Filter reading</center> | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''9:45'''</center> | ||
+ | | <center>'''10:30'''</center> | ||
+ | | <center>'''LECTURE 13'''</center> | ||
+ | | <center>Optical sectioning </center> | ||
+ | |||
+ | <center>Confocal Microscopy</center> | ||
+ | |||
+ | <center>2 photon microscopy</center> | ||
+ | |||
+ | <center>Spinning disk</center> | ||
+ | |||
+ | <center>TIRF</center> | ||
+ | | # | ||
+ | # | ||
+ | # Jan | ||
+ | |||
+ | |||
+ | |||
+ | |- | ||
+ | | <center>'''10:30'''</center> | ||
+ | | <center>'''12:00'''</center> | ||
+ | | <center>'''ACTIVITY 13'''</center> | ||
+ | | System rotation ---Confocal-2P- Spinning disk | ||
+ | | Pete – Jan - Britta | ||
+ | |||
+ | |- | ||
+ | | <center>'''12:00'''</center> | ||
+ | | <center>'''13:00'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>LUNCH</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''13:00'''</center> | ||
+ | | <center>'''14:30'''</center> | ||
+ | | <center>'''ACTIVITY 13'''</center> | ||
+ | | System rotation ---Confocal-2P- Spinning disk | ||
+ | | Pete – Jan - Britta | ||
+ | |||
+ | |- | ||
+ | | <center>'''14:30'''</center> | ||
+ | | <center>'''16:00'''</center> | ||
+ | | <center>'''ACTIVITY 13'''</center> | ||
+ | | System rotation ---Confocal-2P- Spinning disk | ||
+ | | <center>Pete – Jan - Britta</center> | ||
+ | |||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | {| class="prettytable" | ||
+ | | <center>'''FROM'''</center> | ||
+ | | <center>'''TO'''</center> | ||
+ | | <center>'''ACTIVITY'''</center> | ||
+ | | <center>'''FRIDAY (Dec 5)'''</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''9:00'''</center> | ||
+ | | '''10:00''' | ||
+ | | <center>'''INTRODUCTION'''</center> | ||
+ | | <center>Questions</center> | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''10:00'''</center> | ||
+ | | '''11:30''' | ||
+ | | <center>'''LECTURE 14'''</center> | ||
+ | | <center>Image processing, Digital images</center> | ||
+ | |||
+ | <center>What is image processing</center> | ||
+ | | <center>Dan</center> | ||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 14'''</center> | ||
+ | | Quantitative imaging + Image processing | ||
+ | | # | ||
+ | |||
+ | |||
+ | |||
+ | |- | ||
+ | | <center>'''11:30'''</center> | ||
+ | | <center>'''11:45'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>Break</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''11:45'''</center> | ||
+ | | '''13:00''' | ||
+ | | <center>'''LECTURE 14'''</center> | ||
+ | |||
+ | <center>'''(continuation)'''</center> | ||
+ | | <center>Image processing </center> | ||
+ | | Dan | ||
+ | |||
+ | |- | ||
+ | | <center>'''ACTIVITY 14'''</center> | ||
+ | |||
+ | <center>'''(continuation)'''</center> | ||
+ | | Image processing - Image J (FIJI) | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''13:00'''</center> | ||
+ | | <center>'''14:00'''</center> | ||
+ | | <center>'''BREAK'''</center> | ||
+ | | <center>LUNCH</center> | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | <center>'''14:00'''</center> | ||
+ | | <center>'''15:30'''</center> | ||
+ | | | ||
+ | | <center>Introduction to LMF - Wiki</center> | ||
+ | |||
+ | <center>Final Discussion - Quiz </center> | ||
+ | | <center>LMF TEAM</center> | ||
+ | |||
+ | |} | ||
[[category:teaching]] | [[category:teaching]] |
Revision as of 15:19, 8 October 2009
Peter Evennett's Course Oct 09
Preliminary Schedule
Friday 16th Oct
Setup - all lmf staff move stuff to galleria Equipment needed
- microscopes
- optocal bench
- spinning disk gadget
|
|
|
|
|
|
|
|
|
|
|
|
|||
|
|
|
|
|
|
|
|
|
|
|
# 1. [Capillary + oil] + Coin in glass
2. Laser refraction + reflection
|
# 1. Peter
2. Humberto
| ||
|
|
|
|
|
|
|
|
|
|
|
|
|||
|
# 1. Finding focal point of lenses + infinity + Color fringes
|
# 1. Jan
| ||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
10:00 |
|
|
|
|
|
|
Numerical aperture |
|
|
# Diffraction grids/gratings
|
#
| ||
|
# Airy disk on the microscope on mirror
|
# LMF TEAM
| ||
|
|
|
|
|
11:15 | |
|
|
|
|
|
|
# Objective cleaning + NA reading + Cover slip thickness measurement
|
# Pete
|
|
|
|
|
|
|
|
|
*
|
Humberto |
|
Dark field
Phase contrast |
| ||
|
|
|
|
|
|
|
|
*
|
Humberto |
|
Polarization – Polarization foils – Crystals
DIC |
|
|
|
|
|
|
|
10:00 | |
|
|
|
|
|
|
|
|
|
ACTIVITY 10 | |
# 1. Humberto
2. Britta 3. Jan |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Britta |
|
|
|
|
|
|
|
|
|
Dan |
|
|
|
Mercury lamp exchange and centering | |
|
|
|
Wide fields systems + CCD
Fluorescence
|
|
|
|
|
|
|
|
9:45 |
|
|
|
|
|
|
|
#
|
|
|
|
System rotation ---Confocal-2P- Spinning disk | Pete – Jan - Britta |
|
|
|
|
|
|
|
|
System rotation ---Confocal-2P- Spinning disk | Pete – Jan - Britta |
|
|
|
System rotation ---Confocal-2P- Spinning disk | |
|
|
|
|
|
|
10:00 | |
|
|
|
11:30 | |
|
|
|
Quantitative imaging + Image processing | #
| ||
|
|
|
|
|
|
13:00 | |
|
Dan |
|
Image processing - Image J (FIJI) | |||
|
|
|
|
|
|
|
|
|