|
|
Line 10: |
Line 10: |
| * spinning disk gadget | | * spinning disk gadget |
| | | |
− | | + | {| {{table}} |
− | | + | | align="center" style="background:#f0f0f0;"|'''FROM''' |
− | <center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
| + | | align="center" style="background:#f0f0f0;"|'''TO''' |
− | | + | | align="center" style="background:#f0f0f0;"|'''ACTIVITY''' |
− | <center>'''Week 2 '''</center>
| + | | align="center" style="background:#f0f0f0;"|'''MONDAY (Dec 1)''' |
− | | + | | align="center" style="background:#f0f0f0;"|'''Responsible person(s)''' |
− | | + | |
− | | + | |
− | {| class="prettytable" | + | |
− | | <center>'''FROM'''</center> | + | |
− | | <center>'''TO'''</center> | + | |
− | | <center>'''ACTIVITY'''</center> | + | |
− | | <center>'''MONDAY (Dec 1)'''</center> | + | |
− | | <center>'''Responsible person(s)'''</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''9:00'''</center> | + | | ||||||Group presentation|| |
− | | <center>'''10:30'''</center> | + | |
− | | <center>'''INTRODUCTION'''</center> | + | |
− | | + | |
− | | + | |
− | | + | |
− | | <center>Group presentation</center> | + | |
− | | + | |
− | <center>Wishes for the course</center>
| + | |
− | | + | |
− | <center>The ideal microscope</center>
| + | |
− | | <center>LMF TEAM</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''DEMO'''</center> | + | | 9:00||10:30||INTRODUCTION||Wishes for the course||LMF TEAM |
− | | <center>Optical bench</center> | + | |
− | | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''10:30'''</center> | + | | ||||||The ideal microscope|| |
− | | <center>'''11:00'''</center> | + | |
− | | <center>'''BREAK'''</center> | + | |
− | | <center>Break</center> | + | |
− | | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''11:00'''</center> | + | | ||||DEMO||Optical bench|| |
− | | + | |
− | | + | |
− | | + | |
− | | <center>'''12:30'''</center> | + | |
− | | <center>'''LECTURE 1'''</center> | + | |
− | | <center>Basics in Optics</center> | + | |
− | | + | |
− | <center>Characteristics of light</center>
| + | |
− | | + | |
− | <center>Light sources</center>
| + | |
− | | + | |
− | <center>Reflection, Absorption, Refraction</center>
| + | |
− | | + | |
− | <center>Diffraction (Demo)</center>
| + | |
− | | <center>Humberto</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''ACTIVITY 1'''</center> | + | | 10:30||11:00||BREAK||Break|| |
− | | + | |
− | <center>'''(Group Rotation)'''</center>
| + | |
− | | # <nowiki>1. [Capillary + oil] + </nowiki>Coin in glass | + | |
− | | + | |
− | 2. Laser refraction + reflection
| + | |
− | | + | |
− | # 3. Different objectives ‚Äì Demo TIRF Objective (immersion solution)
| + | |
− | | + | |
− | | + | |
− | | # 1. Peter | + | |
− | | + | |
− | 2. Humberto
| + | |
− | | + | |
− | # 3. Britta
| + | |
− | | + | |
− | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''12:30'''</center> | + | | ||||||Basics in Optics|| |
− | | <center>'''13:30'''</center> | + | |
− | | <center>'''BREAK'''</center> | + | |
− | | <center>LUNCH</center> | + | |
− | | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''13:30'''</center> | + | | ||||||Characteristics of light|| |
− | | <center>'''15:30'''</center> | + | |
− | | <center>'''LECTURE 2'''</center> | + | |
− | | <center>Introduction to lenses</center> | + | |
− | | + | |
− | <center>Lens aberrations</center>
| + | |
− | | + | |
− | <center>Understanding ray optics</center>
| + | |
− | | <center>Humberto</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''ACTIVITY 2'''</center> | + | | ||||LECTURE 1||Light sources||Humberto |
− | | <center>Ray optics</center> | + | |
− | | + | |
− | <center>Drawing ray graphics/diagrams</center>
| + | |
− | | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''ACTIVITY 3'''</center> | + | | 11:00||12:30||||Reflection, Absorption, Refraction|| |
− | | # 1. Finding focal point of lenses + infinity + Color fringes | + | |
− | # 2. Virtual & real images. 25cm distance
| + | |
− | # 3. Image of the arrow in the microscope (upright ‚Äì inverted)
| + | |
− | | + | |
− | | + | |
− | | # 1. Jan | + | |
− | # 2. Humberto
| + | |
− | # 3. Pete + Britta
| + | |
− | | + | |
− | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''15:30'''</center> | + | | |||||| Diffraction (Demo)|| |
− | | <center>'''15:50'''</center> | + | |
− | | <center>'''BREAK'''</center> | + | |
− | | <center>Break</center> | + | |
− | | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''15:50'''</center> | + | | ||||ACTIVITY 1||1. 1. [Capillary + oil] + Coin in glass||3. 1. Peter |
− | | <center>'''17:00'''</center> | + | |
− | | <center>'''LECTURE 3'''</center> | + | |
− | | <center>Components of the microscope</center> | + | |
− | | + | |
− | <center>Focusing, Field of view, Depth of field, depth of focus</center>
| + | |
− | | + | |
− | <center>Planes (filament DEMO)</center>
| + | |
− | | + | |
− | <center>Conjugated planes</center>
| + | |
− | | + | |
− | <center>Koehler illumination</center>
| + | |
− | | <center>Humberto</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''17:00'''</center> | + | | ||||(Group Rotation)|| 2. Laser refraction + reflection|| 2. Humberto |
− | | <center>'''18:30'''</center> | + | |
− | | <center>'''ACTIVITY 4'''</center> | + | |
− | | + | |
− | <center>'''Auditorium + Room 036'''</center>
| + | |
− | | <center>Glass cube demo and practice - Koehler alignment + Document</center> | + | |
− | | <center>LMF TEAM</center> | + | |
− | | + | |
− | |} | + | |
− | | + | |
− | | + | |
− | | + | |
− | <center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
| + | |
− | | + | |
− | <center>'''Week 2 '''</center>
| + | |
− | | + | |
− | | + | |
− | {| class="prettytable"
| + | |
− | | <center>'''FROM'''</center> | + | |
− | | <center>'''TO'''</center>
| + | |
− | | <center>'''ACTIVITY'''</center>
| + | |
− | | <center>'''TUESDAY (Dec 2)'''</center>
| + | |
− | | <center>'''Responsible person(s)'''</center>
| + | |
− | | + | |
| |- | | |- |
− | | <center>'''9:00'''</center> | + | | ||||||2. 3. Different objectives – Demo TIRF Objective (immersion solution)|| 3. Britta |
− | | '''10:00''' | + | |
− | | <center>'''INTRODUCTION'''</center> | + | |
− | | + | |
− | | + | |
− | | + | |
− | | <center>Koehler exercise</center> | + | |
− | | + | |
− | <center>Questions</center>
| + | |
− | | <center>LMF TEAM</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''10:00'''</center> | + | | 12:30||13:30||BREAK||LUNCH|| |
− | | <center>'''11:00'''</center> | + | |
− | | <center>'''LECTURE 4'''</center> | + | |
− | | <center>Diffraction </center> | + | |
− | | + | |
− | Numerical aperture
| + | |
− | | <center>Humberto</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''ACTIVITY 5'''</center> | + | | ||||||Introduction to lenses|| |
− | | # Diffraction grids/gratings | + | |
− | | + | |
− | | + | |
− | | # | + | |
− | | + | |
− | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''ACTIVITY 6'''</center> | + | | ||||LECTURE 2||Lens aberrations||Humberto |
− | | # Airy disk on the microscope on mirror | + | |
− | | + | |
− | | + | |
− | | # LMF TEAM | + | |
− | | + | |
− | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''11:00'''</center> | + | | ||||||Understanding ray optics|| |
− | | <center>'''11:15'''</center> | + | |
− | | <center>'''BREAK'''</center> | + | |
− | | <center>Break</center> | + | |
− | | | + | |
− | | + | |
| |- | | |- |
− | | '''11:15''' | + | | 13:30||15:30||ACTIVITY 2||Ray optics|| |
− | | <center>'''11:30'''</center> | + | |
− | | <center>'''LECTURE 5'''</center> | + | |
− | | <center>Apertures (Objective and Condenser) - Cube DEMO</center> | + | |
− | | <center>Humberto</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''11:30'''</center> | + | | ||||||Drawing ray graphics/diagrams|| |
− | | <center>'''13:00'''</center> | + | |
− | | <center>'''ACTIVITY 7'''</center> | + | |
− | | + | |
− | | + | |
− | | + | |
− | | # Objective cleaning + NA reading + Cover slip thickness measurement | + | |
− | # Diffraction exercise on microscope
| + | |
− | # Fluorescent beads with variable NA exercise
| + | |
− | | + | |
− | | + | |
− | | # Pete | + | |
− | # Dan
| + | |
− | # Britta
| + | |
− | | + | |
− | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''13:00'''</center> | + | | ||||ACTIVITY 3||1. 1. Finding focal point of lenses + infinity + Color fringes||4. 1. Jan |
− | | <center>'''14:00'''</center> | + | |
− | | <center>'''BREAK'''</center> | + | |
− | | <center>LUNCH</center> | + | |
− | | | + | |
− | | + | |
| |- | | |- |
− | | <center>'''14:00'''</center> | + | | ||||||2. 2. Virtual & real images. 25cm distance||5. 2. Humberto |
− | | <center>'''15:45'''</center> | + | |
− | | <center>'''LECTURE 6'''</center> | + | |
− | | * <center><nowiki>Contrasting techniques [Dark field - Phase contrast]</nowiki></center> | + | |
− | | + | |
− | | + | |
− | | Humberto | + | |
− | | + | |
| |- | | |- |
− | | <center>'''ACTIVITY 8'''</center> | + | | ||||||3. 3. Image of the arrow in the microscope (upright – inverted)||6. 3. Pete + Britta |
− | | Dark field | + | |
− | | + | |
− | Phase contrast
| + | |
− | | <center>LMF TEAM</center> | + | |
− | | + | |
− | |- | + | |
− | | <center>'''15:45'''</center> | + | |
− | | <center>'''16:00'''</center> | + | |
− | | <center>'''BREAK'''</center> | + | |
− | | <center>Break</center>
| + | |
− | |
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''16:00'''</center>
| + | |
− | | <center>'''18:00'''</center>
| + | |
− | | <center>'''LECTURE 7'''</center>
| + | |
− | | * <center><nowiki>Contrasting techniques [Polarization ‚Äì DIC]</nowiki></center>
| + | |
− | | + | |
− | | + | |
− | | Humberto
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''ACTIVITY 9'''</center>
| + | |
− | | Polarization ‚Äì Polarization foils ‚Äì Crystals
| + | |
− | | + | |
− | DIC
| + | |
− | | <center>LMF TEAM</center>
| + | |
− | | + | |
− | |}
| + | |
− | | + | |
− | | + | |
− | | + | |
− | <center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
| + | |
− | | + | |
− | <center>'''Week 2 '''</center>
| + | |
− | | + | |
− | | + | |
− | | + | |
− | {| class="prettytable"
| + | |
− | | <center>'''FROM'''</center>
| + | |
− | | <center>'''TO'''</center>
| + | |
− | | <center>'''ACTIVITY'''</center>
| + | |
− | | <center>'''WEDNESDAY (Dec 3)'''</center>
| + | |
− | | <center>'''Responsible person(s)'''</center>
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''9:00'''</center>
| + | |
− | | '''10:00'''
| + | |
− | | <center>'''INTRODUCTION'''</center>
| + | |
− | | <center>Questions</center>
| + | |
− | | <center>LMF TEAM</center>
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''10:00'''</center>
| + | |
− | | <center>'''11:25'''</center>
| + | |
− | | <center>'''LECTURE 8'''</center>
| + | |
− | | + | |
− | <center>'''(Interactive session)'''</center>
| + | |
− | | <center>Objectives</center>
| + | |
− | | + | |
− | <center>Lens aberrations</center>
| + | |
− | | + | |
− | <center>Infinity optics </center>
| + | |
− | | <center>Humberto</center>
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''11:25'''</center>
| + | |
− | | <center>'''11:15'''</center>
| + | |
− | | '''ACTIVITY 10'''
| + | |
− | | <center>1. Lens Aberrations</center>
| + | |
− | | + | |
− | <center>2. Objective reading</center>
| + | |
− | | + | |
− | <center>3. Infinity optics on optical bench</center>
| + | |
− | | # 1. Humberto
| + | |
− | | + | |
− | 2. Britta
| + | |
− | | + | |
− | 3. Jan
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''11:15'''</center>
| + | |
− | | <center>'''11:30'''</center>
| + | |
− | | <center>'''BREAK'''</center>
| + | |
− | | <center>Break</center>
| + | |
− | |
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''11:30'''</center>
| + | |
− | | <center>'''13:00'''</center>
| + | |
− | | <center>'''LECTURE 9'''</center>
| + | |
− | | <center>Basics of fluorescence</center>
| + | |
− | | + | |
− | <center>Fluorochromes</center>
| + | |
− | | + | |
− | <center>The fluorescence microscope</center>
| + | |
− | | + | |
− | <center>Filters</center>
| + | |
− | | <center>Humberto</center>
| + | |
− | | + | |
− | <center>Dan</center>
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''13:00'''</center>
| + | |
− | | <center>'''14:00'''</center>
| + | |
− | | <center>'''BREAK'''</center>
| + | |
− | | <center>LUNCH</center>
| + | |
− | |
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''14:00'''</center>
| + | |
− | | <center>'''15:00'''</center>
| + | |
− | | <center>'''LECTURE 11'''</center>
| + | |
− | | <center>CCD - DEMO</center>
| + | |
− | | Britta
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''15:00'''</center>
| + | |
− | | <center>'''15:15'''</center>
| + | |
− | | <center>'''BREAK'''</center>
| + | |
− | | <center>Break</center>
| + | |
− | |
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''15:15'''</center>
| + | |
− | | <center>'''16:15'''</center>
| + | |
− | | <center>'''LECTURE 12'''</center>
| + | |
− | | <center>Digital imaging (live cell imaging ‚Äì optional)</center>
| + | |
− | | Dan | + | |
− | | + | |
− | |- | + | |
− | | <center>'''16:15'''</center>
| + | |
− | | <center>'''16:45'''</center>
| + | |
− | | <center>'''ACTIVITY 11'''</center>
| + | |
− | | Mercury lamp exchange and centering
| + | |
− | | <center>LMF TEAM</center>
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''16:45'''</center>
| + | |
− | | <center>'''18:30'''</center>
| + | |
− | | <center>'''ACTIVITY 12'''</center>
| + | |
− | | Wide fields systems + CCD
| + | |
− | | + | |
− | Fluorescence
| + | |
− | | + | |
− | | + | |
− | | + | |
− | | <center>LMF TEAM</center>
| + | |
− | | + | |
− | |}
| + | |
− | | + | |
− | | + | |
− | | + | |
− | <center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
| + | |
− | | + | |
− | <center>'''Week 2 '''</center>
| + | |
− | | + | |
− | | + | |
− | {| class="prettytable"
| + | |
− | | <center>'''FROM'''</center>
| + | |
− | | <center>'''TO'''</center>
| + | |
− | | <center>'''ACTIVITY'''</center>
| + | |
− | | <center>'''THURSDAY (Dec 4 )'''</center>
| + | |
− | |
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''9:00'''</center>
| + | |
− | | '''9:45'''
| + | |
− | | <center>'''INTRODUCTION'''</center>
| + | |
− | | + | |
− | | + | |
− | | + | |
− | | <center>Questions</center>
| + | |
− | | + | |
− | <center>Filter reading</center>
| + | |
− | | <center>LMF TEAM</center>
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''9:45'''</center>
| + | |
− | | <center>'''10:30'''</center>
| + | |
− | | <center>'''LECTURE 13'''</center>
| + | |
− | | <center>Optical sectioning </center>
| + | |
− | | + | |
− | <center>Confocal Microscopy</center>
| + | |
− | | + | |
− | <center>2 photon microscopy</center>
| + | |
− | | + | |
− | <center>Spinning disk</center>
| + | |
− | | + | |
− | <center>TIRF</center>
| + | |
− | | #
| + | |
− | #
| + | |
− | # Jan
| + | |
− | | + | |
− | | + | |
− | | + | |
− | |-
| + | |
− | | <center>'''10:30'''</center>
| + | |
− | | <center>'''12:00'''</center>
| + | |
− | | <center>'''ACTIVITY 13'''</center>
| + | |
− | | System rotation ---Confocal-2P- Spinning disk
| + | |
− | | Pete ‚Äì Jan - Britta
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''12:00'''</center>
| + | |
− | | <center>'''13:00'''</center>
| + | |
− | | <center>'''BREAK'''</center>
| + | |
− | | <center>LUNCH</center>
| + | |
− | |
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''13:00'''</center>
| + | |
− | | <center>'''14:30'''</center>
| + | |
− | | <center>'''ACTIVITY 13'''</center>
| + | |
− | | System rotation ---Confocal-2P- Spinning disk
| + | |
− | | Pete ‚Äì Jan - Britta
| + | |
− | | + | |
− | |-
| + | |
− | | <center>'''14:30'''</center>
| + | |
− | | <center>'''16:00'''</center>
| + | |
− | | <center>'''ACTIVITY 13'''</center>
| + | |
− | | System rotation ---Confocal-2P- Spinning disk
| + | |
− | | <center>Pete ‚Äì Jan - Britta</center>
| + | |
− | | + | |
− | |}
| + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | {| class="prettytable"
| + | |
− | | <center>'''FROM'''</center>
| + | |
− | | <center>'''TO'''</center>
| + | |
− | | <center>'''ACTIVITY'''</center>
| + | |
− | | <center>'''FRIDAY (Dec 5)'''</center>
| + | |
− | |
| + | |
− | | + | |
| |- | | |- |
− | | <center>'''9:00'''</center> | + | | 15:30||15:50||BREAK||Break|| |
− | | '''10:00''' | + | |
− | | <center>'''INTRODUCTION'''</center> | + | |
− | | <center>Questions</center> | + | |
− | | <center>LMF TEAM</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''10:00'''</center> | + | | 15:50||17:00||LECTURE 3||Components of the microscope|| |
− | | '''11:30''' | + | |
− | | <center>'''LECTURE 14'''</center> | + | |
− | | <center>Image processing, Digital images</center> | + | |
− | | + | |
− | <center>What is image processing</center>
| + | |
− | | <center>Dan</center> | + | |
− | | + | |
| |- | | |- |
− | | <center>'''ACTIVITY 14'''</center> | + | | ||||||Focusing, Field of view, Depth of field, depth of focus||Humberto |
− | | Quantitative imaging + Image processing | + | |
− | | # | + | |
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− | | <center>'''11:30'''</center> | + | | ||||||Planes (filament DEMO)|| |
− | | <center>'''11:45'''</center> | + | |
− | | <center>'''BREAK'''</center> | + | |
− | | <center>Break</center> | + | |
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− | | <center>'''11:45'''</center> | + | | ||||||Conjugated planes|| |
− | | '''13:00''' | + | |
− | | <center>'''LECTURE 14'''</center> | + | |
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− | <center>'''(continuation)'''</center>
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− | | <center>Image processing </center> | + | |
− | | Dan | + | |
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− | | <center>'''ACTIVITY 14'''</center> | + | | ||||||Koehler illumination|| |
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− | <center>'''(continuation)'''</center>
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− | | Image processing - Image J (FIJI) | + | |
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− | | <center>'''13:00'''</center> | + | | 17:00||18:30||ACTIVITY 4||Glass cube demo and practice - Koehler alignment + Document||LMF TEAM |
− | | <center>'''14:00'''</center> | + | |
− | | <center>'''BREAK'''</center> | + | |
− | | <center>LUNCH</center> | + | |
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− | | <center>'''14:00'''</center> | + | | ||||Auditorium + Room 036|||| |
− | | <center>'''15:30'''</center> | + | |
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− | | <center>Introduction to LMF - Wiki</center> | + | |
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− | <center>Final Discussion - Quiz </center>
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− | | <center>LMF TEAM</center> | + | |
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| |} | | |} |
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| [[category:teaching]] | | [[category:teaching]] |