Peter Evennett's Course Oct 09
From BioDIP
(Difference between revisions)
(→DAY TWO (October 20th)) |
|||
Line 28: | Line 28: | ||
| |||||||| | | |||||||| | ||
|- | |- | ||
− | | 9:30|| | + | | 9:30||11:05||align="center"|INTRODUCTION||align="center" | COURSE PRESENTATION <br> GROUP PRESENTATION||align="center" |LMF TEAM |
|- | |- | ||
| |||||||| | | |||||||| | ||
|- | |- | ||
|- | |- | ||
+ | | style="background:DarkKhaki;"|'''11:05''' | ||
+ | | style="background:DarkKhaki;"|'''11:20''' | ||
+ | |align="center" style="background:DarkKhaki;"|'''BREAK''' | ||
+ | |align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|''' ''' | ||
+ | |- | ||
+ | | |||||||| | ||
+ | |- | ||
+ | | | ||
| 10:00||12:30||align="center"|SESSION 1 <br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Historical aspects of microscopy<br>Introduction to lenses<br>Lens aberrations<br>Magnification<br> Numerical aperture<br>Microscope illumination<br>Conjugate planes<br>Koehler illumination||align="center" |PETER EVENNETT <br>LMF TEAM | | 10:00||12:30||align="center"|SESSION 1 <br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Historical aspects of microscopy<br>Introduction to lenses<br>Lens aberrations<br>Magnification<br> Numerical aperture<br>Microscope illumination<br>Conjugate planes<br>Koehler illumination||align="center" |PETER EVENNETT <br>LMF TEAM | ||
|- | |- | ||
Line 134: | Line 142: | ||
| |||||||||||| | | |||||||||||| | ||
|- | |- | ||
− | | 9:30||10:00||align="center"|SESSION 1<br>Theory||align="center" | Fundamentals concepts of fluorescence||align="center" |LMF TEAM<br>Dan||align="center" |- ||align"center" | + | | 9:30||10:00||align="center"|SESSION 1<br>Theory||align="center" | Fundamentals concepts of fluorescence||align="center" |LMF TEAM<br>Dan||align="center" |- ||align"center" |
|- | |- | ||
| 10:00||12:30||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Humberto||align="center" |• Mercury lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions | | 10:00||12:30||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Humberto||align="center" |• Mercury lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions | ||
Line 203: | Line 211: | ||
|- | |- | ||
| 9:30||12:30||align="center"| - ||align="center" | Britta ||align="center" |Peter ||align="center" |Peter ||align="center" |Silke | | 9:30||12:30||align="center"| - ||align="center" | Britta ||align="center" |Peter ||align="center" |Peter ||align="center" |Silke | ||
− | |||
|- | |- | ||
| style="background:DarkKhaki;"|'''12:30''' | | style="background:DarkKhaki;"|'''12:30''' |
Revision as of 11:21, 26 October 2009
Contents |
Peter Evennett's Course Oct 09
Preliminary Schedule
Friday 16th Oct
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
DAY ONE (October 19th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | |
9:30 | 11:05 | INTRODUCTION | COURSE PRESENTATION GROUP PRESENTATION |
LMF TEAM | |
11:05 | 11:20 | BREAK | LUNCH | ||
10:00 | 12:30 | SESSION 1 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Historical aspects of microscopy Introduction to lenses Lens aberrations Magnification Numerical aperture Microscope illumination Conjugate planes Koehler illumination |
PETER EVENNETT LMF TEAM | |
12:30 | 13:30 | BREAK | LUNCH | ||
13:30 | 18:00 | SESSION 2 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY | PETER EVENNETT LMF TEAM | |
DAY TWO (October 20th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:30 | 11:00 | SESSION 1 Theory & Practice |
Resolution Diffraction |
PETER EVENNETT LMF TEAM |
11:00 | 11:15 | BREAK | COFFEE BREAK | |
11:15 | 13:00 | DEMONSTRATION | Abbe´s diffraction experiments |
PETER EVENNETT LMF TEAM |
13:00 | 14:00 | BREAK | LUNCH | |
14:00 | 18:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM |
DAY THREE (October 21th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:30 | 12:30 | SESSION 1 Theory & Practice |
Polarized light microscopy Differential Interference Contrast (DIC) |
PETER EVENNETT LMF TEAM |
12:30 | 13:30 | BREAK | LUNCH | |
13:30 | 18:00 | SESSION 2 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
DAY FOUR (October 22th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | DEMO Activities | DEMO materials |
9:30 | 10:00 | SESSION 1 Theory |
Fundamentals concepts of fluorescence | LMF TEAM Dan |
- | align"center" |
10:00 | 12:30 | SESSION 2 Theory & Practice |
Fluorescence Microscopy | LMF TEAM Humberto |
• Mercury lamp • Lamp houses • Spectra • Filters • Filter cubes • Fluorescent-labeled samples • Filter spectral charts of each microscope (laminated) |
• Spare lamp houses • Lamps • Ocean optics equipment • Different filters • Four (4) different Filter-sets (cubes), with the appropriate descriptions |
12:30 | 13:30 | BREAK | LUNCH | |||
13:30 | 15:00 | SESSION 3 Theory & Practice |
Imaging with CCD | LMF TEAM Britta |
• CCD • Cameras |
• CCD chip • Cameras |
15:00 | 15:15 | BREAK | COFFEE BREAK | |||
15:15 | 18:00 | SESSION 4 Practice |
Imaging with CCD cameras using LMF systems | LMF TEAM Jan / Peter / Dan / Britta (*) |
• Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast |
• Microscopes (4) • Sample slides |
DAY FIVE (October 23th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:30 | 12:30 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
12:30 | 13:30 | BREAK | LUNCH | |
13:30 | 14:30 | SESSION 2 Theory & Demo |
Basics of Quantitative Image Analysis (Image Processing) |
LMF TEAM Dan |
14:30 | 15:15 | SESSION 3 | Course evaluation | LMF TEAM |
OFFICE DUTIES
- LMF Members available for users (19th Oct to 23rd Oct) -
FROM | TO | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
9:30 | 12:30 | - | Britta | Peter | Peter | Silke |
12:30 | 13:30 | L | U | N | C | H |
13:30 | 18:00 | Britta | Dan | Silke | (*)Silke Humberto |
- |
(*) Silke and Humberto will be cleaning and reorganizing the Galeria