Peter Evennett's Course Oct 09
From BioDIP
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+ | | 10:55||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Humberto||align="center" |• Mercury lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions | ||
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− | | | + | | 14:15||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD ||align="center" |LMF TEAM<br> Britta||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • Cameras |
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− | | | + | | 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM<br>Jan / Peter / Dan / Britta (*)||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes (4) <br>• Sample slides |
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Revision as of 18:43, 26 October 2009
Contents |
Peter Evennett's Course Oct 09
Preliminary Schedule
Friday 16th Oct
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
DAY ONE (October 19th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:30 | 11:05 | INTRODUCTION | COURSE PRESENTATION GROUP PRESENTATION |
LMF TEAM |
11:05 | 11:20 | BREAK | BREAK | BREAK |
11:20 | 13:00 | SESSION 1 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Historical aspects of microscopy Introduction to lenses Lens aberrations Magnification Numerical aperture Microscope illumination Conjugate planes Koehler illumination |
PETER EVENNETT LMF TEAM |
13:00 | 14:00 | LUNCH | LUNCH | LUNCH |
14:00 | 15:30 | SESSION 2 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Demo |
PETER EVENNETT LMF TEAM |
15:30 | 15:45 | BREAK | BREAK | BREAK |
15:45 | 18:00 | SESSION 3 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY | PETER EVENNETT LMF TEAM |
DAY TWO (October 20th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:30 | 10:45 | SESSION 1 Theory & Practice |
Resolution Diffraction |
PETER EVENNETT LMF TEAM |
10:45 | 11:00 | BREAK | COFFEE BREAK | |
11:00 | 13:00 | DEMONSTRATION | Abbe´s diffraction experiments |
PETER EVENNETT LMF TEAM |
13:00 | 14:00 | BREAK | LUNCH | |
14:00 | 16:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM |
16:00 | 16:15 | BREAK | COFFEE BREAK | |
18:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM | |
DAY THREE (October 21th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:30 | 11:30 | SESSION 1 Theory & Practice |
Objective reading Objective cleaning |
PETER EVENNETT LMF TEAM |
11:30 | 11:45 | BREAK | BREAK | BREAK |
11:45 | 13:00 | SESSION 2 Theory & Practice |
Polarized light microscopy Differential Interference Contrast (DIC) |
PETER EVENNETT LMF TEAM |
13:00 | 14:00 | BREAK | LUNCH | |
14:00 | 15:15 | SESSION 3 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
15:15 | 15:30 | BREAK | BREAK | BREAK |
15:30 | 18:00 | SESSION 4 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
DAY FOUR (October 22th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | DEMO Activities | DEMO materials |
9:30 | 10:20 | SESSION 1 Theory |
Question session Fundamentals concepts of fluorescence |
LMF TEAM Dan |
- | - |
10:20 | 10:55 | BREAK | BREAK | BREAK | BREAK | BREAK |
10:55 | 13:15 | SESSION 2 Theory & Practice |
Fluorescence Microscopy | LMF TEAM Humberto |
• Mercury lamp • Lamp houses • Spectra • Filters • Filter cubes • Fluorescent-labeled samples • Filter spectral charts of each microscope (laminated) |
• Spare lamp houses • Lamps • Ocean optics equipment • Different filters • Four (4) different Filter-sets (cubes), with the appropriate descriptions |
13:15 | 14:15 | BREAK | LUNCH | LUNCH | LUNCH | LUNCH |
14:15 | 15:45 | SESSION 3 Theory & Practice |
Imaging with CCD | LMF TEAM Britta |
• CCD • Cameras |
• CCD chip • Cameras |
15:45 | 16:00 | BREAK | BREAK | |||
16:00 | 18:00 | SESSION 4 Practice |
Imaging with CCD cameras using LMF systems | LMF TEAM Jan / Peter / Dan / Britta (*) |
• Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast |
• Microscopes (4) • Sample slides |
DAY FIVE (October 23th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:30 | 12:30 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
12:30 | 13:30 | BREAK | LUNCH | |
13:30 | 14:30 | SESSION 2 Theory & Demo |
Basics of Quantitative Image Analysis (Image Processing) |
LMF TEAM Dan |
14:30 | 15:15 | SESSION 3 | Course evaluation | LMF TEAM |
OFFICE DUTIES
- LMF Members available for users (19th Oct to 23rd Oct) -
FROM | TO | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
9:30 | 12:30 | - | Britta | Peter | Peter | Silke |
12:30 | 13:30 | L | U | N | C | H |
13:30 | 18:00 | Britta | Dan | Silke | (*)Silke Humberto |
- |
(*) Silke and Humberto will be cleaning and reorganizing the Galeria