Peter Evennett's Course Oct 09

From BioDIP
(Difference between revisions)
Jump to: navigation, search
(changed image processing to quantitative image analysis)
 
(45 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
==Peter Evennett's Course Oct 09 ==
 
==Peter Evennett's Course Oct 09 ==
  
−
== Preliminary Schedule ==
 
 
=== Friday 16th Oct ===
 
=== Friday 16th Oct ===
 
Setup - all lmf staff move stuff to galleria
 
Setup - all lmf staff move stuff to galleria
Line 28: Line 27:
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
| 9:30||9:45||align="center"|INTRODUCTION||align="center" | COURSE PRESENTATION <br> GROUP PRESENTATION||align="center" |LMF TEAM
+
| 9:30||11:05||align="center"|INTRODUCTION||align="center" | COURSE PRESENTATION <br> GROUP PRESENTATION||align="center" |LMF TEAM
 
|-
 
|-
 
| ||||||||
 
| ||||||||
 
|-
 
|-
 
|-
 
|-
−
| 9:45||12:30||align="center"|SESSION 1 <br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Historical aspects of microscopy<br>Resolution<br>Diffraction<br>Introduction to lenses<br>Magnification||align="center" |PETER EVENNETT <br>LMF TEAM
+
| style="background:DarkKhaki;"|'''11:05'''
 +
| style="background:DarkKhaki;"|'''11:20'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
 
| ||||||||
 
| ||||||||
 
|-
 
|-
 +
| 11:20||13:00||align="center"|SESSION 1 <br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Historical aspects of microscopy<br>Introduction to lenses<br>Lens aberrations<br>Magnification<br> Numerical aperture<br>Microscope illumination<br>Conjugate planes<br>Koehler illumination||align="center" |PETER EVENNETT <br>LMF TEAM
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''12:30'''
+
| ||||||||
−
| style="background:DarkKhaki;"|'''13:30'''
+
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''13:00'''
 +
| style="background:DarkKhaki;"|'''14:00'''
 +
|align="center" style="background:DarkKhaki;"|'''LUNCH'''
 +
|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 +
|-
 +
| ||||||||
 +
|-
 +
| 14:00||15:30||align="center"|SESSION 2<br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY <br> Demo||align="center" |PETER EVENNETT <br>LMF TEAM
 +
|-
 +
| ||||||||
 +
|-
 +
| style="background:DarkKhaki;"|'''15:30'''
 +
| style="background:DarkKhaki;"|'''15:45'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''     '''
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
| 13:30||18:00||align="center"|SESSION 2<br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Numerical aperture<br>Microscope illumination<br>Conjugated planes<br>Koehler illumination||align="center" |PETER EVENNETT <br>LMF TEAM
+
| 15:45||18:00||align="center"|SESSION 3<br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY||align="center" |PETER EVENNETT <br>LMF TEAM
 
|-
 
|-
 
| ||||||||
 
| ||||||||
 
|}
 
|}
−
 
  
 
=== DAY TWO (October 20th)===
 
=== DAY TWO (October 20th)===
Line 63: Line 79:
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
| 9:30||10:30||align="center"|DEMONSTRATION||align="center" |  Abbe´s diffraction experiments<br>  ||align="center" |PETER EVENNETT <br>LMF TEAM
+
| 9:30||10:45||align="center"|SESSION 1 <br>Theory & Practice||align="center" |  Resolution<br>Diffraction ||align="center" |PETER EVENNETT <br>LMF TEAM
 
|-
 
|-
 
| ||||||||
 
| ||||||||
Line 69: Line 85:
 
|--
 
|--
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''10:30'''
 
 
| style="background:DarkKhaki;"|'''10:45'''
 
| style="background:DarkKhaki;"|'''10:45'''
 +
| style="background:DarkKhaki;"|'''11:00'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
|align="center" style="background:DarkKhaki;"|'''COFFE BREAK'''|| style="background:DarkKhaki;"|'''    '''
+
|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''    '''
 
|-
 
|-
−
| 10:45||12:30||align="center"|SESSION 1 <br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
+
| 11:00||13:00||align="center"|DEMONSTRATION || align="center"|Abbe´s diffraction experiments<br>||align="center"|PETER EVENNETT <br>LMF TEAM
 
|-
 
|-
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
|-
+
| style="background:DarkKhaki;"|'''13:00'''
−
| style="background:DarkKhaki;"|'''12:30'''
+
| style="background:DarkKhaki;"|'''14:00'''
−
| style="background:DarkKhaki;"|'''13:30'''
+
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
Line 86: Line 101:
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
| 13:30||18:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
+
| 14:00||16:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''16:00'''
 +
| style="background:DarkKhaki;"|'''16:15'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''    '''
 +
|-
 +
| 16:15|18:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
 
|-
 
|-
 
| ||||||||
 
| ||||||||
Line 103: Line 126:
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
| 9:30||12:30||align="center"|DEMO||align="center" | Polarized light microscopy<br>Differential Interference Contrast (DIC)||align="center" |PETER EVENNETT <br>LMF TEAM
+
| 9:30||11:30||align="center"|SESSION 1<br>Theory & Practice||align="center" | Objective reading<br> Objective cleaning||align="center" |PETER EVENNETT <br>LMF TEAM
 
|-
 
|-
 
| ||||||||
 
| ||||||||
−
 
 
|-
 
|-
 +
| style="background:DarkKhaki;"|'''11:30'''
 +
| style="background:DarkKhaki;"|'''11:45'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''12:30'''
+
| ||||||||
−
| style="background:DarkKhaki;"|'''13:30'''
+
|-
 +
| 11:45||13:00||align="center"|SESSION 2<br>Theory & Practice||align="center" | Polarized light microscopy<br>Differential Interference Contrast (DIC)||align="center" |PETER EVENNETT <br>LMF TEAM
 +
|-
 +
| ||||||||
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''13:00'''
 +
| style="background:DarkKhaki;"|'''14:00'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
Line 116: Line 149:
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
| 13:30||18:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Differential Interference Contrast (DIC)<br> Digital photomicrography ||align="center" |PETER EVENNETT <br>LMF TEAM
+
| 14:00||15:15||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Differential Interference Contrast (DIC)<br>Objective cleaning<br>Digital photomicrography<br>Introduction to Confocal Microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
−
 
+
|-
 +
| ||||||||
 +
|-
 +
| style="background:DarkKhaki;"|'''15:15'''
 +
| style="background:DarkKhaki;"|'''15:30'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
| ||||||||
 +
|-
 +
| 15:30||18:00||align="center"|SESSION 4<br>Theory & Practice|| align="center"|Differential Interference Contrast (DIC)<br>Objective cleaning<br>Digital photomicrography<br>Introduction to Confocal Microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
 
|-
 
|-
 
| ||||||||
 
| ||||||||
Line 131: Line 174:
 
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 +
| align="center" style="background:#f0f0f0;"|'''DEMO Activities'''
 +
| align="center" style="background:#f0f0f0;"|'''DEMO materials'''
 
|-
 
|-
−
| ||||||||
+
| ||||||||||||
 
|-
 
|-
−
| 9:30||10:00||align="center"|SESSION 1||align="center" | Fundamentals concepts of fluorescence||align="center" |LMF TEAM
+
| 9:30||10:20||align="center"|SESSION 1<br>Theory||align="center" | Question session <br> Fundamentals concepts of fluorescence ||align="center" |LMF TEAM<br>Dan||align="center" |- ||align="center" |-
 
|-
 
|-
−
| 10:00||12:30||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM
+
| ||||||||||||
 
|-
 
|-
−
| ||||||||
+
| style="background:DarkKhaki;"|'''10:20'''
 +
| style="background:DarkKhaki;"|'''10:55'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
| ||||||||||||
 +
|-
 +
| 10:55||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Humberto||align="center" |• Mercury lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions
 +
|-
 +
| ||||||||||||
  
 
|-
 
|-
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''12:30'''
+
| style="background:DarkKhaki;"|'''13:15'''
−
| style="background:DarkKhaki;"|'''13:30'''
+
| style="background:DarkKhaki;"|'''14:15'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''     '''
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 
|-
 
|-
−
| ||||||||
+
| ||||||||||||
 
|-
 
|-
−
| 13:30||15:00||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD ||align="center" |LMF TEAM
+
| 14:15||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD ||align="center" |LMF TEAM<br> Britta||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • Cameras
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''15:00'''
+
| style="background:DarkKhaki;"|'''15:45'''
−
| style="background:DarkKhaki;"|'''15:15'''
+
| style="background:DarkKhaki;"|'''16:00'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
|align="center" style="background:DarkKhaki;"|'''COFFE BREAK'''|| style="background:DarkKhaki;"|'''    '''
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''
 
|-
 
|-
−
| 13:30||15:00||align="center"|SESSION 4<br>Theory & Practice|| align="center"|Imaging with CCD ||align="center" |LMF TEAM
+
| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM<br>Jan / Peter / Dan / Britta (*)||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes (4) <br>• Sample slides
 
|}
 
|}
 +
''
  
 
=== DAY FIVE (October 23th)===
 
=== DAY FIVE (October 23th)===
Line 168: Line 223:
 
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 +
|-
 +
| 9:30||10:20||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
 
|-
 
|-
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
| 9:30||12:30||align="center"|SESSION 1||align="center" | Optical sectioning ||align="center" |LMF TEAM
+
| style="background:DarkKhaki;"|'''10:20'''
 +
| style="background:DarkKhaki;"|'''10:35'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
 +
| ||||||||
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''12:30'''
+
| 10:35||13:00||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
−
| style="background:DarkKhaki;"|'''13:30'''
+
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''13:00'''
 +
| style="background:DarkKhaki;"|'''14:00'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
Line 181: Line 245:
 
| ||||||||
 
| ||||||||
 
|-
 
|-
−
| 13:30||14:30||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Quantitative Image Analysis <br> (Image Processing) ||align="center" |LMF TEAM
+
| 14:00||15:15||align="center"|SESSION 2<br>Theory & Demo|| align="center"|Basics of Quantitative Image Analysis <br> (Image Processing) ||align="center" |LMF TEAM<br>Dan
 +
|-
 +
| style="background:DarkKhaki;"|'''15:15'''
 +
| style="background:DarkKhaki;"|'''15:30'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
| ||||||||
 +
|-
 +
| 15:30||16:30||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM
 +
|}
 +
 
 +
=== OFFICE DUTIES===
 +
==== - LMF Members available for users (19th Oct to 23rd Oct) - ====
 +
 
 +
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 +
|- style="; color:MidnightBlue;"
 +
| align="center" style="background:#f0f0f0;"|'''FROM'''
 +
| align="center" style="background:#f0f0f0;"|'''TO'''
 +
| align="center" style="background:#f0f0f0;"|'''MONDAY'''
 +
| align="center" style="background:#f0f0f0;"|'''TUESDAY'''
 +
| align="center" style="background:#f0f0f0;"|'''WEDNESDAY'''
 +
| align="center" style="background:#f0f0f0;"|'''THURSDAY'''
 +
| align="center" style="background:#f0f0f0;"|'''FRIDAY'''
 +
|-
 +
| |||||||| ||||
 +
|-
 +
| 9:30||12:30||align="center"| - ||align="center" | Britta ||align="center" |Peter ||align="center" |Peter ||align="center" |Silke
 +
|-
 +
| style="background:DarkKhaki;"|'''12:30'''
 +
| style="background:DarkKhaki;"|'''13:30'''
 +
|align="center" style="background:DarkKhaki;"|'''L'''
 +
|align="center" style="background:DarkKhaki;"|'''U'''|| align="center" style="background:DarkKhaki;"|'''  N  '''||align="center" style="background:DarkKhaki;"|'''  C  '''||align="center" style="background:DarkKhaki;"|'''  H  '''
 +
|-
 +
| ||||||||||||
 
|-
 
|-
−
| 14:30||15:15||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM
+
| 13:30||18:00||align="center"|Britta|| align="center"|Dan ||align="center" |Silke ||align="center" |(*)Silke <br> Humberto||align="center" |-
 
|}
 
|}
 +
(*) Silke and Humberto will be cleaning and reorganizing the Galeria

Latest revision as of 15:50, 6 November 2009

Contents

[edit] Peter Evennett's Course Oct 09

[edit] Friday 16th Oct

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


[edit] DAY ONE (October 19th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:05 INTRODUCTION COURSE PRESENTATION
GROUP PRESENTATION		 LMF TEAM
11:05 11:20 BREAK BREAK BREAK
11:20 13:00 SESSION 1
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects of microscopy
Introduction to lenses
Lens aberrations
Magnification
Numerical aperture
Microscope illumination
Conjugate planes
Koehler illumination		 PETER EVENNETT
LMF TEAM
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:30 SESSION 2
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY
Demo		 PETER EVENNETT
LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 18:00 SESSION 3
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY PETER EVENNETT
LMF TEAM

[edit] DAY TWO (October 20th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:45 SESSION 1
Theory & Practice		 Resolution
Diffraction 		PETER EVENNETT
LMF TEAM
10:45 11:00 BREAK COFFEE BREAK
11:00 13:00 DEMONSTRATION Abbe´s diffraction experiments
 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 16:00 SESSION 2
Theory & Practice		 Bright Field
Dark Field
Phase Contrast microscopy 		PETER EVENNETT
LMF TEAM
16:00 16:15 BREAK COFFEE BREAK
18:00 SESSION 2
Theory & Practice		 Bright Field
Dark Field
Phase Contrast microscopy 		PETER EVENNETT
LMF TEAM

[edit] DAY THREE (October 21th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:30 SESSION 1
Theory & Practice		 Objective reading
Objective cleaning		 PETER EVENNETT
LMF TEAM
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 SESSION 2
Theory & Practice		 Polarized light microscopy
Differential Interference Contrast (DIC)		 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 3
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM
15:15 15:30 BREAK BREAK BREAK
15:30 18:00 SESSION 4
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM

[edit] DAY FOUR (October 22th)

FROM TO ACTIVITY TOPICS Responsible person(s) DEMO Activities DEMO materials
9:30 10:20 SESSION 1
Theory		 Question session
Fundamentals concepts of fluorescence 		LMF TEAM
Dan		 - -
10:20 10:55 BREAK BREAK BREAK BREAK BREAK
10:55 13:15 SESSION 2
Theory & Practice		 Fluorescence Microscopy LMF TEAM
Humberto		 • Mercury lamp
• Lamp houses
• Spectra
• Filters
• Filter cubes
• Fluorescent-labeled samples
• Filter spectral charts of each microscope (laminated)		 • Spare lamp houses
• Lamps
• Ocean optics equipment
• Different filters
• Four (4) different Filter-sets (cubes), with the appropriate descriptions
13:15 14:15 BREAK LUNCH LUNCH LUNCH LUNCH
14:15 15:45 SESSION 3
Theory & Practice		 Imaging with CCD LMF TEAM
Britta		 • CCD
• Cameras 		• CCD chip
• Cameras
15:45 16:00 BREAK BREAK
16:00 18:00 SESSION 4
Practice		 Imaging with CCD cameras using LMF systems LMF TEAM
Jan / Peter / Dan / Britta (*)		 • Displaying
• Pixel size
• Look up table
• Binning
• Exposure time
• Saturation
• Signal to noise
• ROI
• Bit depth
• Auto map
• Auto contrast 		• Microscopes (4)
• Sample slides

[edit] DAY FIVE (October 23th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:20 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
10:20 10:35 BREAK BREAK BREAK
10:35 13:00 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 2
Theory & Demo		 Basics of Quantitative Image Analysis
(Image Processing) 		LMF TEAM
Dan
15:15 15:30 BREAK BREAK BREAK
15:30 16:30 SESSION 3 Course evaluation LMF TEAM

[edit] OFFICE DUTIES

[edit] - LMF Members available for users (19th Oct to 23rd Oct) -

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
9:30 12:30 - Britta Peter Peter Silke
12:30 13:30 L U N C H
13:30 18:00 Britta Dan Silke (*)Silke
Humberto		 -

(*) Silke and Humberto will be cleaning and reorganizing the Galeria

Retrieved from "https://www.biodip.de/w/index.php?title=Peter_Evennett%27s_Course_Oct_09&oldid=4312"
Personal tools