Peter Evennett's Course Oct 09

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==Peter Evennett's Course Oct 09 ==
 
==Peter Evennett's Course Oct 09 ==
  
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== Preliminary Schedule ==
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=== Friday 16th Oct ===
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== Friday 16th Oct ==
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Setup - all lmf staff move stuff to galleria
 
Setup - all lmf staff move stuff to galleria
 
Equipment needed
 
Equipment needed
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* microscopes
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* Microscopes
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* optocal bench
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* Optical bench
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* spinning disk gadget
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* Spinning disk gadget
 +
* Polarazing sheets
 +
* Diffraction Grids
 +
* Fluorescent samples
 +
* Fluorescent Beads (Prepare slides)
 +
* First day Handout for students
 +
* Light torches
  
  
 +
=== DAY ONE (October 19th)===
  
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<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
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{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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|- style="; color:MidnightBlue;"
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<center>'''Week 2 '''</center>
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| align="center" style="background:#f0f0f0;"|'''FROM'''
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| align="center" style="background:#f0f0f0;"|'''TO'''
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| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
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| align="center" style="background:#f0f0f0;"|'''TOPICS'''
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{| class="prettytable"
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| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
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| <center>'''FROM'''</center>
+
−
| <center>'''TO'''</center>
+
−
| <center>'''ACTIVITY'''</center>
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| <center>'''MONDAY (Dec 1)'''</center>
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| <center>'''Responsible person(s)'''</center>
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+
 
|-
 
|-
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| <center>'''9:00'''</center>
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| ||||||||
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| <center>'''10:30'''</center>
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−
| <center>'''INTRODUCTION'''</center>
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+
−
 
+
−
 
+
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| <center>Group presentation</center>
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+
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<center>Wishes for the course</center>
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<center>The ideal microscope</center>
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| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
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| <center>'''DEMO'''</center>
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| 9:30||11:05||align="center"|INTRODUCTION||align="center" | COURSE PRESENTATION <br> GROUP PRESENTATION||align="center" |LMF TEAM
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| <center>Optical bench</center>
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−
|  
+
−
 
+
 
|-
 
|-
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| <center>'''10:30'''</center>
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| ||||||||
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| <center>'''11:00'''</center>
+
−
| <center>'''BREAK'''</center>
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| <center>Break</center>
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−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''11:00'''</center>
 
−
 
−
 
−
 
−
| <center>'''12:30'''</center>
 
−
| <center>'''LECTURE 1'''</center>
 
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| <center>Basics in Optics</center>
 
−
 
−
<center>Characteristics of light</center>
 
−
 
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<center>Light sources</center>
 
−
 
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<center>Reflection, Absorption, Refraction</center>
 
−
 
−
<center>Diffraction (Demo)</center>
 
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| <center>Humberto</center>
 
−
 
 
|-
 
|-
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| <center>'''ACTIVITY 1'''</center>
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| style="background:DarkKhaki;"|'''11:05'''
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| style="background:DarkKhaki;"|'''11:20'''
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<center>'''(Group Rotation)'''</center>
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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| # <nowiki>1. [Capillary + oil] + </nowiki>Coin in glass
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
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+
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2. Laser refraction + reflection
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+
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# 3. Different objectives – Demo TIRF Objective (immersion solution)
+
−
 
+
−
 
+
−
| # 1. Peter
+
−
 
+
−
2. Humberto
+
−
 
+
−
# 3. Britta
+
−
 
+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''12:30'''</center>
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| ||||||||
−
| <center>'''13:30'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>LUNCH</center>
+
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''13:30'''</center>
+
| 11:20||13:00||align="center"|SESSION 1 <br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Historical aspects of microscopy<br>Introduction to lenses<br>Lens aberrations<br>Magnification<br> Numerical aperture<br>Microscope illumination<br>Conjugate planes<br>Koehler illumination||align="center" |PETER EVENNETT <br>LMF TEAM
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| <center>'''15:30'''</center>
+
−
| <center>'''LECTURE 2'''</center>
+
−
| <center>Introduction to lenses</center>
+
−
 
+
−
<center>Lens aberrations</center>
+
−
 
+
−
<center>Understanding ray optics</center>
+
−
| <center>Humberto</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 2'''</center>
+
| ||||||||
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| <center>Ray optics</center>
+
−
 
+
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<center>Drawing ray graphics/diagrams</center>
+
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 3'''</center>
 
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| # 1. Finding focal point of lenses + infinity + Color fringes
 
−
# 2. Virtual & real images. 25cm distance
 
−
# 3. Image of the arrow in the microscope (upright – inverted)
 
−
 
−
 
−
| # 1. Jan
 
−
# 2. Humberto
 
−
# 3. Pete + Britta
 
−
 
−
 
−
 
 
|-
 
|-
−
| <center>'''15:30'''</center>
+
| style="background:DarkKhaki;"|'''13:00'''
−
| <center>'''15:50'''</center>
+
| style="background:DarkKhaki;"|'''14:00'''
−
| <center>'''BREAK'''</center>
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''
−
| <center>Break</center>
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''15:50'''</center>
+
| ||||||||
−
| <center>'''17:00'''</center>
+
−
| <center>'''LECTURE 3'''</center>
+
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| <center>Components of the microscope</center>
+
−
 
+
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<center>Focusing, Field of view, Depth of field, depth of focus</center>
+
−
 
+
−
<center>Planes (filament DEMO)</center>
+
−
 
+
−
<center>Conjugated planes</center>
+
−
 
+
−
<center>Koehler illumination</center>
+
−
| <center>Humberto</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''17:00'''</center>
+
| 14:00||15:30||align="center"|SESSION 2<br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY <br> Demo||align="center" |PETER EVENNETT <br>LMF TEAM
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| <center>'''18:30'''</center>
+
|-
−
| <center>'''ACTIVITY 4'''</center>
+
| ||||||||
−
 
+
|-
−
<center>'''Auditorium + Room 036'''</center>
+
| style="background:DarkKhaki;"|'''15:30'''
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| <center>Glass cube demo and practice - Koehler alignment + Document</center>
+
| style="background:DarkKhaki;"|'''15:45'''
−
| <center>LMF TEAM</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
 
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
| ||||||||
 +
|-
 +
| 15:45||18:00||align="center"|SESSION 3<br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY||align="center" |PETER EVENNETT <br>LMF TEAM
 +
|-
 +
| ||||||||
 
|}
 
|}
  
 +
=== DAY TWO (October 20th)===
  
−
 
+
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
−
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
+
|- style="; color:MidnightBlue;"
−
 
+
| align="center" style="background:#f0f0f0;"|'''FROM'''
−
<center>'''Week 2 '''</center>
+
| align="center" style="background:#f0f0f0;"|'''TO'''
−
 
+
| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
−
 
+
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
−
{| class="prettytable"
+
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
−
| <center>'''FROM'''</center>
+
−
| <center>'''TO'''</center>
+
−
| <center>'''ACTIVITY'''</center>
+
−
| <center>'''TUESDAY (Dec 2)'''</center>
+
−
| <center>'''Responsible person(s)'''</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''9:00'''</center>
+
| ||||||||
−
| '''10:00'''
+
−
| <center>'''INTRODUCTION'''</center>
+
−
 
+
−
 
+
−
 
+
−
| <center>Koehler exercise</center>
+
−
 
+
−
<center>Questions</center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''10:00'''</center>
+
| 9:30||10:45||align="center"|SESSION 1 <br>Theory & Practice||align="center" | Resolution<br>Diffraction ||align="center" |PETER EVENNETT <br>LMF TEAM
−
| <center>'''11:00'''</center>
+
−
| <center>'''LECTURE 4'''</center>
+
−
| <center>Diffraction </center>
+
−
 
+
−
Numerical aperture
+
−
| <center>Humberto</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 5'''</center>
+
| ||||||||
−
| # Diffraction grids/gratings
+
−
 
+
−
 
+
−
| #
+
−
 
+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 6'''</center>
+
|--
−
| # Airy disk on the microscope on mirror
+
−
 
+
−
 
+
−
| # LMF TEAM
+
−
 
+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''11:00'''</center>
+
| style="background:DarkKhaki;"|'''10:45'''
−
| <center>'''11:15'''</center>
+
| style="background:DarkKhaki;"|'''11:00'''
−
| <center>'''BREAK'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| <center>Break</center>
+
|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''    '''
−
|  
+
−
 
+
 
|-
 
|-
−
| '''11:15'''
+
| 11:00||13:00||align="center"|DEMONSTRATION || align="center"|Abbe´s diffraction experiments<br>||align="center"|PETER EVENNETT <br>LMF TEAM
−
| <center>'''11:30'''</center>
+
−
| <center>'''LECTURE 5'''</center>
+
−
| <center>Apertures (Objective and Condenser) - Cube DEMO</center>
+
−
| <center>Humberto</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''11:30'''</center>
+
| ||||||||
−
| <center>'''13:00'''</center>
+
−
| <center>'''ACTIVITY 7'''</center>
+
−
 
+
−
 
+
−
 
+
−
| # Objective cleaning + NA reading + Cover slip thickness measurement
+
−
# Diffraction exercise on microscope
+
−
# Fluorescent beads with variable NA exercise
+
−
 
+
−
 
+
−
| # Pete
+
−
# Dan
+
−
# Britta
+
−
 
+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''13:00'''</center>
+
| style="background:DarkKhaki;"|'''13:00'''
−
| <center>'''14:00'''</center>
+
| style="background:DarkKhaki;"|'''14:00'''
−
| <center>'''BREAK'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| <center>LUNCH</center>
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''14:00'''</center>
+
| ||||||||
−
| <center>'''15:45'''</center>
+
−
| <center>'''LECTURE 6'''</center>
+
−
| * <center><nowiki>Contrasting techniques [Dark field - Phase contrast]</nowiki></center>
+
−
 
+
−
 
+
−
| Humberto
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 8'''</center>
+
| 14:00||16:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
−
| Dark field
+
−
 
+
−
Phase contrast
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''15:45'''</center>
 
−
| <center>'''16:00'''</center>
 
−
| <center>'''BREAK'''</center>
 
−
| <center>Break</center>
 
−
|
 
−
 
 
|-
 
|-
−
| <center>'''16:00'''</center>
+
| style="background:DarkKhaki;"|'''16:00'''
−
| <center>'''18:00'''</center>
+
| style="background:DarkKhaki;"|'''16:15'''
−
| <center>'''LECTURE 7'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| * <center><nowiki>Contrasting techniques [Polarization – DIC]</nowiki></center>
+
|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''    '''
−
 
+
−
 
+
−
| Humberto
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 9'''</center>
+
| 16:15|18:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
−
| Polarization – Polarization foils – Crystals
+
|-
−
 
+
| ||||||||
−
DIC
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|}
 
|}
  
 +
=== DAY THREE (October 21th)===
  
−
 
+
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
−
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
+
|- style="; color:MidnightBlue;"
−
 
+
| align="center" style="background:#f0f0f0;"|'''FROM'''
−
<center>'''Week 2 '''</center>
+
| align="center" style="background:#f0f0f0;"|'''TO'''
−
 
+
| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
−
 
+
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
−
 
+
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
−
{| class="prettytable"
+
−
| <center>'''FROM'''</center>
+
−
| <center>'''TO'''</center>
+
−
| <center>'''ACTIVITY'''</center>
+
−
| <center>'''WEDNESDAY (Dec 3)'''</center>
+
−
| <center>'''Responsible person(s)'''</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''9:00'''</center>
+
| ||||||||
−
| '''10:00'''
+
−
| <center>'''INTRODUCTION'''</center>
+
−
| <center>Questions</center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''10:00'''</center>
+
| 9:30||11:30||align="center"|SESSION 1<br>Theory & Practice||align="center" | Objective reading<br> Objective cleaning||align="center" |PETER EVENNETT <br>LMF TEAM
−
| <center>'''11:25'''</center>
+
−
| <center>'''LECTURE 8'''</center>
+
−
 
+
−
<center>'''(Interactive session)'''</center>
+
−
| <center>Objectives</center>
+
−
 
+
−
<center>Lens aberrations</center>
+
−
 
+
−
<center>Infinity optics </center>
+
−
| <center>Humberto</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''11:25'''</center>
+
| ||||||||
−
| <center>'''11:15'''</center>
+
−
| '''ACTIVITY 10'''
+
−
| <center>1. Lens Aberrations</center>
+
−
 
+
−
<center>2. Objective reading</center>
+
−
 
+
−
<center>3. Infinity optics on optical bench</center>
+
−
| # 1. Humberto
+
−
 
+
−
2. Britta
+
−
 
+
−
3. Jan
+
−
 
+
 
|-
 
|-
−
| <center>'''11:15'''</center>
+
| style="background:DarkKhaki;"|'''11:30'''
−
| <center>'''11:30'''</center>
+
| style="background:DarkKhaki;"|'''11:45'''
−
| <center>'''BREAK'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| <center>Break</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''11:30'''</center>
+
| ||||||||
−
| <center>'''13:00'''</center>
+
−
| <center>'''LECTURE 9'''</center>
+
−
| <center>Basics of fluorescence</center>
+
−
 
+
−
<center>Fluorochromes</center>
+
−
 
+
−
<center>The fluorescence microscope</center>
+
−
 
+
−
<center>Filters</center>
+
−
| <center>Humberto</center>
+
−
 
+
−
<center>Dan</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''13:00'''</center>
+
| 11:45||13:00||align="center"|SESSION 2<br>Theory & Practice||align="center" | Polarized light microscopy<br>Differential Interference Contrast (DIC)||align="center" |PETER EVENNETT <br>LMF TEAM
−
| <center>'''14:00'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>LUNCH</center>
+
−
|
+
−
 
+
 
|-
 
|-
−
| <center>'''14:00'''</center>
+
| ||||||||
−
| <center>'''15:00'''</center>
+
−
| <center>'''LECTURE 11'''</center>
+
−
| <center>CCD - DEMO</center>
+
−
| Britta
+
−
 
+
 
|-
 
|-
−
| <center>'''15:00'''</center>
 
−
| <center>'''15:15'''</center>
 
−
| <center>'''BREAK'''</center>
 
−
| <center>Break</center>
 
−
|
 
−
 
 
|-
 
|-
−
| <center>'''15:15'''</center>
+
| style="background:DarkKhaki;"|'''13:00'''
−
| <center>'''16:15'''</center>
+
| style="background:DarkKhaki;"|'''14:00'''
−
| <center>'''LECTURE 12'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| <center>Digital imaging (live cell imaging – optional)</center>
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
−
| Dan
+
−
 
+
 
|-
 
|-
−
| <center>'''16:15'''</center>
+
| ||||||||
−
| <center>'''16:45'''</center>
+
−
| <center>'''ACTIVITY 11'''</center>
+
−
| Mercury lamp exchange and centering
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''16:45'''</center>
+
| 14:00||15:15||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Differential Interference Contrast (DIC)<br>Objective cleaning<br>Digital photomicrography<br>Introduction to Confocal Microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
−
| <center>'''18:30'''</center>
+
|-
−
| <center>'''ACTIVITY 12'''</center>
+
| ||||||||
−
| Wide fields systems + CCD
+
|-
−
 
+
| style="background:DarkKhaki;"|'''15:15'''
−
Fluorescence
+
| style="background:DarkKhaki;"|'''15:30'''
−
 
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
 
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
−
 
+
|-
−
| <center>LMF TEAM</center>
+
| ||||||||
−
 
+
|-
 +
| 15:30||18:00||align="center"|SESSION 4<br>Theory & Practice|| align="center"|Differential Interference Contrast (DIC)<br>Objective cleaning<br>Digital photomicrography<br>Introduction to Confocal Microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
 +
|-
 +
| ||||||||
 
|}
 
|}
  
 +
=== DAY FOUR (October 22th)===
  
−
 
+
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
−
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
+
|- style="; color:MidnightBlue;"
−
 
+
| align="center" style="background:#f0f0f0;"|'''FROM'''
−
<center>'''Week 2 '''</center>
+
| align="center" style="background:#f0f0f0;"|'''TO'''
−
 
+
| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
−
 
+
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
−
{| class="prettytable"
+
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
−
| <center>'''FROM'''</center>
+
| align="center" style="background:#f0f0f0;"|'''DEMO Activities'''
−
| <center>'''TO'''</center>
+
| align="center" style="background:#f0f0f0;"|'''DEMO materials'''
−
| <center>'''ACTIVITY'''</center>
+
−
| <center>'''THURSDAY (Dec 4 )'''</center>
+
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''9:00'''</center>
+
| ||||||||||||
−
| '''9:45'''
+
−
| <center>'''INTRODUCTION'''</center>
+
−
 
+
−
 
+
−
 
+
−
| <center>Questions</center>
+
−
 
+
−
<center>Filter reading</center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''9:45'''</center>
+
| 9:30||10:20||align="center"|SESSION 1<br>Theory||align="center" | Question session <br> Fundamentals concepts of fluorescence ||align="center" |LMF TEAM<br>Dan||align="center" |- ||align="center" |-
−
| <center>'''10:30'''</center>
+
−
| <center>'''LECTURE 13'''</center>
+
−
| <center>Optical sectioning </center>
+
−
 
+
−
<center>Confocal Microscopy</center>
+
−
 
+
−
<center>2 photon microscopy</center>
+
−
 
+
−
<center>Spinning disk</center>
+
−
 
+
−
<center>TIRF</center>
+
−
| #
+
−
#
+
−
# Jan
+
−
 
+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''10:30'''</center>
+
| ||||||||||||
−
| <center>'''12:00'''</center>
+
−
| <center>'''ACTIVITY 13'''</center>
+
−
| System rotation ---Confocal-2P- Spinning disk
+
−
| Pete – Jan - Britta
+
−
 
+
 
|-
 
|-
−
| <center>'''12:00'''</center>
+
| style="background:DarkKhaki;"|'''10:20'''
−
| <center>'''13:00'''</center>
+
| style="background:DarkKhaki;"|'''10:55'''
−
| <center>'''BREAK'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| <center>LUNCH</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
−
|  
+
|-
 +
| ||||||||||||
 +
|-
 +
| 10:55||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Humberto||align="center" |• Mercury lamp<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions
 +
|-
 +
| ||||||||||||
  
 
|-
 
|-
−
| <center>'''13:00'''</center>
 
−
| <center>'''14:30'''</center>
 
−
| <center>'''ACTIVITY 13'''</center>
 
−
| System rotation ---Confocal-2P- Spinning disk
 
−
| Pete – Jan - Britta
 
−
 
 
|-
 
|-
−
| <center>'''14:30'''</center>
+
| style="background:DarkKhaki;"|'''13:15'''
−
| <center>'''16:00'''</center>
+
| style="background:DarkKhaki;"|'''14:15'''
−
| <center>'''ACTIVITY 13'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| System rotation ---Confocal-2P- Spinning disk
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
−
| <center>Pete – Jan - Britta</center>
+
|-
−
 
+
| ||||||||||||
 +
|-
 +
| 14:15||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD ||align="center" |LMF TEAM<br> Britta||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • Cameras
 +
|-
 +
| style="background:DarkKhaki;"|'''15:45'''
 +
| style="background:DarkKhaki;"|'''16:00'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''|| style="background:DarkKhaki;"|'''    '''
 +
|-
 +
| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM<br>Jan / Peter / Dan / Britta (*)||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes (4) <br>• Sample slides
 
|}
 
|}
 +
''
  
 +
=== DAY FIVE (October 23th)===
  
−
 
+
{| border="4" cellpadding="2" cellspacing="8" {| {{table}}
−
 
+
|- style="; color:MidnightBlue;"
−
{| class="prettytable"
+
| align="center" style="background:#f0f0f0;"|'''FROM'''
−
| <center>'''FROM'''</center>
+
| align="center" style="background:#f0f0f0;"|'''TO'''
−
| <center>'''TO'''</center>
+
| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
−
| <center>'''ACTIVITY'''</center>
+
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
−
| <center>'''FRIDAY (Dec 5)'''</center>
+
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
−
|
+
−
 
+
 
|-
 
|-
−
| <center>'''9:00'''</center>
+
| 9:30||10:20||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
−
| '''10:00'''
+
−
| <center>'''INTRODUCTION'''</center>
+
−
| <center>Questions</center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''10:00'''</center>
+
| ||||||||
−
| '''11:30'''
+
−
| <center>'''LECTURE 14'''</center>
+
−
| <center>Image processing, Digital images</center>
+
−
 
+
−
<center>What is image processing</center>
+
−
| <center>Dan</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 14'''</center>
+
| style="background:DarkKhaki;"|'''10:20'''
−
| Quantitative imaging + Image processing
+
| style="background:DarkKhaki;"|'''10:35'''
−
| #
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
 
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''11:30'''</center>
+
| ||||||||
−
| <center>'''11:45'''</center>
+
|-
−
| <center>'''BREAK'''</center>
+
| 10:35||13:00||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
−
| <center>Break</center>
+
−
|
+
−
 
+
 
|-
 
|-
−
| <center>'''11:45'''</center>
 
−
| '''13:00'''
 
−
| <center>'''LECTURE 14'''</center>
 
−
 
−
<center>'''(continuation)'''</center>
 
−
| <center>Image processing </center>
 
−
| Dan
 
−
 
 
|-
 
|-
−
| <center>'''ACTIVITY 14'''</center>
+
| style="background:DarkKhaki;"|'''13:00'''
−
 
+
| style="background:DarkKhaki;"|'''14:00'''
−
<center>'''(continuation)'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| Image processing - Image J (FIJI)  
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
−
|  
+
|-
−
 
+
| ||||||||
 +
|-
 +
| 14:00||15:15||align="center"|SESSION 2<br>Theory & Demo|| align="center"|Basics of Quantitative Image Analysis <br> (Image Processing) ||align="center" |LMF TEAM<br>Dan
 
|-
 
|-
−
| <center>'''13:00'''</center>
+
| style="background:DarkKhaki;"|'''15:15'''
−
| <center>'''14:00'''</center>
+
| style="background:DarkKhaki;"|'''15:30'''
−
| <center>'''BREAK'''</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''
−
| <center>LUNCH</center>
+
|align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''14:00'''</center>
+
| ||||||||
−
| <center>'''15:30'''</center>
+
|-
−
|  
+
| 15:30||16:30||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM
−
| <center>Introduction to LMF - Wiki</center>
+
−
 
+
−
<center>Final Discussion - Quiz </center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|}
 
|}
  
 +
=== OFFICE DUTIES===
 +
==== - LMF Members available for users (19th Oct to 23rd Oct) - ====
  
−
 
+
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
−
[[category:teaching]]
+
|- style="; color:MidnightBlue;"
 +
| align="center" style="background:#f0f0f0;"|'''FROM'''
 +
| align="center" style="background:#f0f0f0;"|'''TO'''
 +
| align="center" style="background:#f0f0f0;"|'''MONDAY'''
 +
| align="center" style="background:#f0f0f0;"|'''TUESDAY'''
 +
| align="center" style="background:#f0f0f0;"|'''WEDNESDAY'''
 +
| align="center" style="background:#f0f0f0;"|'''THURSDAY'''
 +
| align="center" style="background:#f0f0f0;"|'''FRIDAY'''
 +
|-
 +
| |||||||| ||||
 +
|-
 +
| 9:30||12:30||align="center"| - ||align="center" | Britta ||align="center" |Peter ||align="center" |Peter ||align="center" |Silke
 +
|-
 +
| style="background:DarkKhaki;"|'''12:30'''
 +
| style="background:DarkKhaki;"|'''13:30'''
 +
|align="center" style="background:DarkKhaki;"|'''L'''
 +
|align="center" style="background:DarkKhaki;"|'''U'''|| align="center" style="background:DarkKhaki;"|'''  N  '''||align="center" style="background:DarkKhaki;"|'''  C  '''||align="center" style="background:DarkKhaki;"|'''  H  '''
 +
|-
 +
| ||||||||||||
 +
|-
 +
| 13:30||18:00||align="center"|Britta|| align="center"|Dan ||align="center" |Silke ||align="center" |(*)Silke <br> Humberto||align="center" |-
 +
|}
 +
(*) Silke and Humberto will be cleaning and reorganizing the Galeria

Latest revision as of 15:50, 6 November 2009

Contents

[edit] Peter Evennett's Course Oct 09

[edit] Friday 16th Oct

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


[edit] DAY ONE (October 19th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:05 INTRODUCTION COURSE PRESENTATION
GROUP PRESENTATION		 LMF TEAM
11:05 11:20 BREAK BREAK BREAK
11:20 13:00 SESSION 1
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects of microscopy
Introduction to lenses
Lens aberrations
Magnification
Numerical aperture
Microscope illumination
Conjugate planes
Koehler illumination		 PETER EVENNETT
LMF TEAM
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:30 SESSION 2
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY
Demo		 PETER EVENNETT
LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 18:00 SESSION 3
Theory & Practice		 PRINCIPLES OF LIGHT MICROSCOPY PETER EVENNETT
LMF TEAM

[edit] DAY TWO (October 20th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:45 SESSION 1
Theory & Practice		 Resolution
Diffraction 		PETER EVENNETT
LMF TEAM
10:45 11:00 BREAK COFFEE BREAK
11:00 13:00 DEMONSTRATION Abbe´s diffraction experiments
 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 16:00 SESSION 2
Theory & Practice		 Bright Field
Dark Field
Phase Contrast microscopy 		PETER EVENNETT
LMF TEAM
16:00 16:15 BREAK COFFEE BREAK
18:00 SESSION 2
Theory & Practice		 Bright Field
Dark Field
Phase Contrast microscopy 		PETER EVENNETT
LMF TEAM

[edit] DAY THREE (October 21th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:30 SESSION 1
Theory & Practice		 Objective reading
Objective cleaning		 PETER EVENNETT
LMF TEAM
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 SESSION 2
Theory & Practice		 Polarized light microscopy
Differential Interference Contrast (DIC)		 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 3
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM
15:15 15:30 BREAK BREAK BREAK
15:30 18:00 SESSION 4
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM

[edit] DAY FOUR (October 22th)

FROM TO ACTIVITY TOPICS Responsible person(s) DEMO Activities DEMO materials
9:30 10:20 SESSION 1
Theory		 Question session
Fundamentals concepts of fluorescence 		LMF TEAM
Dan		 - -
10:20 10:55 BREAK BREAK BREAK BREAK BREAK
10:55 13:15 SESSION 2
Theory & Practice		 Fluorescence Microscopy LMF TEAM
Humberto		 • Mercury lamp
• Lamp houses
• Spectra
• Filters
• Filter cubes
• Fluorescent-labeled samples
• Filter spectral charts of each microscope (laminated)		 • Spare lamp houses
• Lamps
• Ocean optics equipment
• Different filters
• Four (4) different Filter-sets (cubes), with the appropriate descriptions
13:15 14:15 BREAK LUNCH LUNCH LUNCH LUNCH
14:15 15:45 SESSION 3
Theory & Practice		 Imaging with CCD LMF TEAM
Britta		 • CCD
• Cameras 		• CCD chip
• Cameras
15:45 16:00 BREAK BREAK
16:00 18:00 SESSION 4
Practice		 Imaging with CCD cameras using LMF systems LMF TEAM
Jan / Peter / Dan / Britta (*)		 • Displaying
• Pixel size
• Look up table
• Binning
• Exposure time
• Saturation
• Signal to noise
• ROI
• Bit depth
• Auto map
• Auto contrast 		• Microscopes (4)
• Sample slides

[edit] DAY FIVE (October 23th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:20 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
10:20 10:35 BREAK BREAK BREAK
10:35 13:00 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 2
Theory & Demo		 Basics of Quantitative Image Analysis
(Image Processing) 		LMF TEAM
Dan
15:15 15:30 BREAK BREAK BREAK
15:30 16:30 SESSION 3 Course evaluation LMF TEAM

[edit] OFFICE DUTIES

[edit] - LMF Members available for users (19th Oct to 23rd Oct) -

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
9:30 12:30 - Britta Peter Peter Silke
12:30 13:30 L U N C H
13:30 18:00 Britta Dan Silke (*)Silke
Humberto		 -

(*) Silke and Humberto will be cleaning and reorganizing the Galeria

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