Peter Evennett's Course Oct 09

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| align="center" style="background:#f0f0f0;"|'''FROM'''
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<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
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| align="center" style="background:#f0f0f0;"|'''TO'''
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| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
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<center>'''Week 2 '''</center>
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| align="center" style="background:#f0f0f0;"|'''MONDAY (Dec 1)'''
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| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
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{| class="prettytable"
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| <center>'''FROM'''</center>
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| <center>'''TO'''</center>
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| <center>'''ACTIVITY'''</center>
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| <center>'''MONDAY (Dec 1)'''</center>
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| <center>'''Responsible person(s)'''</center>
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|-
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| <center>'''9:00'''</center>
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| ||||||Group presentation||
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| <center>'''10:30'''</center>
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| <center>'''INTRODUCTION'''</center>
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| <center>Group presentation</center>
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<center>Wishes for the course</center>
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<center>The ideal microscope</center>
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| <center>LMF TEAM</center>
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|-
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| <center>'''DEMO'''</center>
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| 9:00||10:30||INTRODUCTION||Wishes for the course||LMF TEAM
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| <center>Optical bench</center>
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|  
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|-
 
|-
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| <center>'''10:30'''</center>
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| ||||||The ideal microscope||
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| <center>'''11:00'''</center>
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| <center>'''BREAK'''</center>
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| <center>Break</center>
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|  
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|-
 
|-
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| <center>'''11:00'''</center>
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| ||||DEMO||Optical bench||
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| <center>'''12:30'''</center>
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| <center>'''LECTURE 1'''</center>
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| <center>Basics in Optics</center>
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<center>Characteristics of light</center>
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<center>Light sources</center>
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<center>Reflection, Absorption, Refraction</center>
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<center>Diffraction (Demo)</center>
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| <center>Humberto</center>
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|-
 
|-
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| <center>'''ACTIVITY 1'''</center>
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| 10:30||11:00||BREAK||Break||
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<center>'''(Group Rotation)'''</center>
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| # <nowiki>1. [Capillary + oil] + </nowiki>Coin in glass
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2. Laser refraction + reflection
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# 3. Different objectives – Demo TIRF Objective (immersion solution)
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| # 1. Peter
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2. Humberto
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# 3. Britta
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|-
 
|-
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| <center>'''12:30'''</center>
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| ||||||Basics in Optics||
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| <center>'''13:30'''</center>
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| <center>'''BREAK'''</center>
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| <center>LUNCH</center>
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|  
+
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|-
 
|-
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| <center>'''13:30'''</center>
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| ||||||Characteristics of light||
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| <center>'''15:30'''</center>
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| <center>'''LECTURE 2'''</center>
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| <center>Introduction to lenses</center>
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+
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<center>Lens aberrations</center>
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−
 
+
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<center>Understanding ray optics</center>
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| <center>Humberto</center>
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|-
 
|-
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| <center>'''ACTIVITY 2'''</center>
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| ||||LECTURE 1||Light sources||Humberto
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| <center>Ray optics</center>
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<center>Drawing ray graphics/diagrams</center>
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|  
+
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|-
 
|-
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| <center>'''ACTIVITY 3'''</center>
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| 11:00||12:30||||Reflection, Absorption, Refraction||
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| # 1. Finding focal point of lenses + infinity + Color fringes
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# 2. Virtual & real images. 25cm distance
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# 3. Image of the arrow in the microscope (upright – inverted)
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+
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+
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| # 1. Jan
+
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# 2. Humberto
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# 3. Pete + Britta
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−
 
+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''15:30'''</center>
+
| |||||| Diffraction (Demo)||
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| <center>'''15:50'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>Break</center>
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−
|  
+
−
 
+
 
|-
 
|-
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| <center>'''15:50'''</center>
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| ||||ACTIVITY 1||1.                      1. [Capillary + oil] +  Coin in glass||3.                      1. Peter
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| <center>'''17:00'''</center>
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| <center>'''LECTURE 3'''</center>
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| <center>Components of the microscope</center>
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<center>Focusing, Field of view, Depth of field, depth of focus</center>
+
−
 
+
−
<center>Planes (filament DEMO)</center>
+
−
 
+
−
<center>Conjugated planes</center>
+
−
 
+
−
<center>Koehler illumination</center>
+
−
| <center>Humberto</center>
+
−
 
+
 
|-
 
|-
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| <center>'''17:00'''</center>
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| ||||(Group Rotation)||      2. Laser refraction + reflection||           2. Humberto
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| <center>'''18:30'''</center>
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| <center>'''ACTIVITY 4'''</center>
+
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+
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<center>'''Auditorium + Room 036'''</center>
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| <center>Glass cube demo and practice - Koehler alignment + Document</center>
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| <center>LMF TEAM</center>
+
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+
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|}
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+
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+
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+
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<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
+
−
 
+
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<center>'''Week 2 '''</center>
+
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+
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+
−
{| class="prettytable"
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| <center>'''FROM'''</center>
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| <center>'''TO'''</center>
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| <center>'''ACTIVITY'''</center>
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| <center>'''TUESDAY (Dec 2)'''</center>
+
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| <center>'''Responsible person(s)'''</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''9:00'''</center>
+
| ||||||2.                      3. Different objectives – Demo TIRF Objective (immersion solution)||    3. Britta
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| '''10:00'''
+
−
| <center>'''INTRODUCTION'''</center>
+
−
 
+
−
 
+
−
 
+
−
| <center>Koehler exercise</center>
+
−
 
+
−
<center>Questions</center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''10:00'''</center>
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| 12:30||13:30||BREAK||LUNCH||
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| <center>'''11:00'''</center>
+
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| <center>'''LECTURE 4'''</center>
+
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| <center>Diffraction </center>
+
−
 
+
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Numerical aperture
+
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| <center>Humberto</center>
+
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+
 
|-
 
|-
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| <center>'''ACTIVITY 5'''</center>
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| ||||||Introduction to lenses||
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| # Diffraction grids/gratings
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+
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+
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| #
+
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+
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+
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+
 
|-
 
|-
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| <center>'''ACTIVITY 6'''</center>
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| ||||LECTURE 2||Lens aberrations||Humberto
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| # Airy disk on the microscope on mirror
+
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+
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+
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| # LMF TEAM
+
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+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''11:00'''</center>
+
| ||||||Understanding ray optics||
−
| <center>'''11:15'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>Break</center>
+
−
|  
+
−
 
+
 
|-
 
|-
−
| '''11:15'''
+
| 13:30||15:30||ACTIVITY 2||Ray optics||
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| <center>'''11:30'''</center>
+
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| <center>'''LECTURE 5'''</center>
+
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| <center>Apertures (Objective and Condenser) - Cube DEMO</center>
+
−
| <center>Humberto</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''11:30'''</center>
+
| ||||||Drawing ray graphics/diagrams||
−
| <center>'''13:00'''</center>
+
−
| <center>'''ACTIVITY 7'''</center>
+
−
 
+
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+
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+
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| # Objective cleaning + NA reading + Cover slip thickness measurement
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# Diffraction exercise on microscope
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# Fluorescent beads with variable NA exercise
+
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+
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+
−
| # Pete
+
−
# Dan
+
−
# Britta
+
−
 
+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''13:00'''</center>
+
| ||||ACTIVITY 3||1.                      1. Finding focal point of lenses + infinity + Color fringes||4.                      1. Jan
−
| <center>'''14:00'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>LUNCH</center>
+
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''14:00'''</center>
+
| ||||||2.                      2. Virtual & real images. 25cm distance||5.                      2. Humberto
−
| <center>'''15:45'''</center>
+
−
| <center>'''LECTURE 6'''</center>
+
−
| * <center><nowiki>Contrasting techniques [Dark field - Phase contrast]</nowiki></center>
+
−
 
+
−
 
+
−
| Humberto
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 8'''</center>
+
| ||||||3.                       3. Image of the arrow in the microscope (upright – inverted)||6.                      3. Pete + Britta
−
| Dark field
+
−
 
+
−
Phase contrast
+
−
| <center>LMF TEAM</center>
+
−
 
+
−
|-
+
−
| <center>'''15:45'''</center>
+
−
| <center>'''16:00'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>Break</center>
+
−
|
+
−
 
+
−
|-
+
−
| <center>'''16:00'''</center>
+
−
| <center>'''18:00'''</center>
+
−
| <center>'''LECTURE 7'''</center>
+
−
| * <center><nowiki>Contrasting techniques [Polarization – DIC]</nowiki></center>
+
−
 
+
−
 
+
−
| Humberto
+
−
 
+
−
|-
+
−
| <center>'''ACTIVITY 9'''</center>
+
−
| Polarization – Polarization foils – Crystals
+
−
 
+
−
DIC
+
−
| <center>LMF TEAM</center>
+
−
 
+
−
|}
+
−
 
+
−
 
+
−
 
+
−
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
+
−
 
+
−
<center>'''Week 2 '''</center>
+
−
 
+
−
 
+
−
 
+
−
{| class="prettytable"
+
−
| <center>'''FROM'''</center>
+
−
| <center>'''TO'''</center>
+
−
| <center>'''ACTIVITY'''</center>
+
−
| <center>'''WEDNESDAY (Dec 3)'''</center>
+
−
| <center>'''Responsible person(s)'''</center>
+
−
 
+
−
|-
+
−
| <center>'''9:00'''</center>
+
−
| '''10:00'''
+
−
| <center>'''INTRODUCTION'''</center>
+
−
| <center>Questions</center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
−
|-
+
−
| <center>'''10:00'''</center>
+
−
| <center>'''11:25'''</center>
+
−
| <center>'''LECTURE 8'''</center>
+
−
 
+
−
<center>'''(Interactive session)'''</center>
+
−
| <center>Objectives</center>
+
−
 
+
−
<center>Lens aberrations</center>
+
−
 
+
−
<center>Infinity optics </center>
+
−
| <center>Humberto</center>
+
−
 
+
−
|-
+
−
| <center>'''11:25'''</center>
+
−
| <center>'''11:15'''</center>
+
−
| '''ACTIVITY 10'''
+
−
| <center>1. Lens Aberrations</center>
+
−
 
+
−
<center>2. Objective reading</center>
+
−
 
+
−
<center>3. Infinity optics on optical bench</center>
+
−
| # 1. Humberto
+
−
 
+
−
2. Britta
+
−
 
+
−
3. Jan
+
−
 
+
−
|-
+
−
| <center>'''11:15'''</center>
+
−
| <center>'''11:30'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>Break</center>
+
−
|
+
−
 
+
−
|-
+
−
| <center>'''11:30'''</center>
+
−
| <center>'''13:00'''</center>
+
−
| <center>'''LECTURE 9'''</center>
+
−
| <center>Basics of fluorescence</center>
+
−
 
+
−
<center>Fluorochromes</center>
+
−
 
+
−
<center>The fluorescence microscope</center>
+
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+
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<center>Filters</center>
+
−
| <center>Humberto</center>
+
−
 
+
−
<center>Dan</center>
+
−
 
+
−
|-
+
−
| <center>'''13:00'''</center>
+
−
| <center>'''14:00'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>LUNCH</center>
+
−
|
+
−
 
+
−
|-
+
−
| <center>'''14:00'''</center>
+
−
| <center>'''15:00'''</center>
+
−
| <center>'''LECTURE 11'''</center>
+
−
| <center>CCD - DEMO</center>
+
−
| Britta
+
−
 
+
−
|-
+
−
| <center>'''15:00'''</center>
+
−
| <center>'''15:15'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>Break</center>
+
−
|
+
−
 
+
−
|-
+
−
| <center>'''15:15'''</center>
+
−
| <center>'''16:15'''</center>
+
−
| <center>'''LECTURE 12'''</center>
+
−
| <center>Digital imaging (live cell imaging – optional)</center>
+
−
| Dan
+
−
 
+
−
|-
+
−
| <center>'''16:15'''</center>
+
−
| <center>'''16:45'''</center>
+
−
| <center>'''ACTIVITY 11'''</center>
+
−
| Mercury lamp exchange and centering
+
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| <center>LMF TEAM</center>
+
−
 
+
−
|-
+
−
| <center>'''16:45'''</center>
+
−
| <center>'''18:30'''</center>
+
−
| <center>'''ACTIVITY 12'''</center>
+
−
| Wide fields systems + CCD
+
−
 
+
−
Fluorescence
+
−
 
+
−
 
+
−
 
+
−
| <center>LMF TEAM</center>
+
−
 
+
−
|}
+
−
 
+
−
 
+
−
 
+
−
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
+
−
 
+
−
<center>'''Week 2 '''</center>
+
−
 
+
−
 
+
−
{| class="prettytable"
+
−
| <center>'''FROM'''</center>
+
−
| <center>'''TO'''</center>
+
−
| <center>'''ACTIVITY'''</center>
+
−
| <center>'''THURSDAY (Dec 4 )'''</center>
+
−
|
+
−
 
+
−
|-
+
−
| <center>'''9:00'''</center>
+
−
| '''9:45'''
+
−
| <center>'''INTRODUCTION'''</center>
+
−
 
+
−
 
+
−
 
+
−
| <center>Questions</center>
+
−
 
+
−
<center>Filter reading</center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
−
|-
+
−
| <center>'''9:45'''</center>
+
−
| <center>'''10:30'''</center>
+
−
| <center>'''LECTURE 13'''</center>
+
−
| <center>Optical sectioning </center>
+
−
 
+
−
<center>Confocal Microscopy</center>
+
−
 
+
−
<center>2 photon microscopy</center>
+
−
 
+
−
<center>Spinning disk</center>
+
−
 
+
−
<center>TIRF</center>
+
−
| #
+
−
#
+
−
# Jan
+
−
 
+
−
 
+
−
 
+
−
|-
+
−
| <center>'''10:30'''</center>
+
−
| <center>'''12:00'''</center>
+
−
| <center>'''ACTIVITY 13'''</center>
+
−
| System rotation ---Confocal-2P- Spinning disk
+
−
| Pete – Jan - Britta
+
−
 
+
−
|-
+
−
| <center>'''12:00'''</center>
+
−
| <center>'''13:00'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>LUNCH</center>
+
−
|
+
−
 
+
−
|-
+
−
| <center>'''13:00'''</center>
+
−
| <center>'''14:30'''</center>
+
−
| <center>'''ACTIVITY 13'''</center>
+
−
| System rotation ---Confocal-2P- Spinning disk
+
−
| Pete – Jan - Britta
+
−
 
+
−
|-
+
−
| <center>'''14:30'''</center>
+
−
| <center>'''16:00'''</center>
+
−
| <center>'''ACTIVITY 13'''</center>
+
−
| System rotation ---Confocal-2P- Spinning disk
+
−
| <center>Pete – Jan - Britta</center>
+
−
 
+
−
|}
+
−
 
+
−
 
+
−
 
+
−
 
+
−
{| class="prettytable"
+
−
| <center>'''FROM'''</center>
+
−
| <center>'''TO'''</center>
+
−
| <center>'''ACTIVITY'''</center>
+
−
| <center>'''FRIDAY (Dec 5)'''</center>
+
−
|
+
−
 
+
 
|-
 
|-
−
| <center>'''9:00'''</center>
+
| 15:30||15:50||BREAK||Break||
−
| '''10:00'''
+
−
| <center>'''INTRODUCTION'''</center>
+
−
| <center>Questions</center>
+
−
| <center>LMF TEAM</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''10:00'''</center>
+
| 15:50||17:00||LECTURE 3||Components of the microscope||
−
| '''11:30'''
+
−
| <center>'''LECTURE 14'''</center>
+
−
| <center>Image processing, Digital images</center>
+
−
 
+
−
<center>What is image processing</center>
+
−
| <center>Dan</center>
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 14'''</center>
+
| ||||||Focusing, Field of view, Depth of field, depth of focus||Humberto
−
| Quantitative imaging + Image processing
+
−
| #
+
−
 
+
−
 
+
−
 
+
 
|-
 
|-
−
| <center>'''11:30'''</center>
+
| ||||||Planes (filament DEMO)||
−
| <center>'''11:45'''</center>
+
−
| <center>'''BREAK'''</center>
+
−
| <center>Break</center>
+
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''11:45'''</center>
+
| ||||||Conjugated planes||
−
| '''13:00'''
+
−
| <center>'''LECTURE 14'''</center>
+
−
 
+
−
<center>'''(continuation)'''</center>
+
−
| <center>Image processing </center>
+
−
| Dan
+
−
 
+
 
|-
 
|-
−
| <center>'''ACTIVITY 14'''</center>
+
| ||||||Koehler illumination||
−
 
+
−
<center>'''(continuation)'''</center>
+
−
| Image processing - Image J (FIJI)
+
−
|  
+
−
 
+
 
|-
 
|-
−
| <center>'''13:00'''</center>
+
| 17:00||18:30||ACTIVITY 4||Glass cube demo and practice  - Koehler alignment  + Document||LMF TEAM
−
| <center>'''14:00'''</center>
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| <center>'''BREAK'''</center>
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| <center>LUNCH</center>
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|  
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|-
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| <center>'''14:00'''</center>
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| ||||Auditorium + Room 036||||
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| <center>'''15:30'''</center>
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| <center>Introduction to LMF - Wiki</center>
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<center>Final Discussion - Quiz </center>
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| <center>LMF TEAM</center>
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[[category:teaching]]
 
[[category:teaching]]

Revision as of 17:05, 8 October 2009

Peter Evennett's Course Oct 09

Preliminary Schedule

Friday 16th Oct

Setup - all lmf staff move stuff to galleria Equipment needed

  • microscopes
  • optocal bench
  • spinning disk gadget
FROM TO ACTIVITY MONDAY (Dec 1) Responsible person(s)
Group presentation
9:00 10:30 INTRODUCTION Wishes for the course LMF TEAM
The ideal microscope
DEMO Optical bench
10:30 11:00 BREAK Break
Basics in Optics
Characteristics of light
LECTURE 1 Light sources Humberto
11:00 12:30 Reflection, Absorption, Refraction
Diffraction (Demo)
ACTIVITY 1 1. 1. [Capillary + oil] + Coin in glass 3. 1. Peter
(Group Rotation) 2. Laser refraction + reflection 2. Humberto
2. 3. Different objectives – Demo TIRF Objective (immersion solution) 3. Britta
12:30 13:30 BREAK LUNCH
Introduction to lenses
LECTURE 2 Lens aberrations Humberto
Understanding ray optics
13:30 15:30 ACTIVITY 2 Ray optics
Drawing ray graphics/diagrams
ACTIVITY 3 1. 1. Finding focal point of lenses + infinity + Color fringes 4. 1. Jan
2. 2. Virtual & real images. 25cm distance 5. 2. Humberto
3. 3. Image of the arrow in the microscope (upright – inverted) 6. 3. Pete + Britta
15:30 15:50 BREAK Break
15:50 17:00 LECTURE 3 Components of the microscope
Focusing, Field of view, Depth of field, depth of focus Humberto
Planes (filament DEMO)
Conjugated planes
Koehler illumination
17:00 18:30 ACTIVITY 4 Glass cube demo and practice - Koehler alignment + Document LMF TEAM
Auditorium + Room 036
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