Peter Evennett's Course Oct 09

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 +
 +
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
 +
 +
<center>'''Week 2 '''</center>
 +
 +
 +
 +
{| class="prettytable"
 +
| <center>'''FROM'''</center>
 +
| <center>'''TO'''</center>
 +
| <center>'''ACTIVITY'''</center>
 +
| <center>'''MONDAY (Dec 1)'''</center>
 +
| <center>'''Responsible person(s)'''</center>
 +
 +
|-
 +
| <center>'''9:00'''</center>
 +
| <center>'''10:30'''</center>
 +
| <center>'''INTRODUCTION'''</center>
 +
 +
 +
 +
| <center>Group presentation</center>
 +
 +
<center>Wishes for the course</center>
 +
 +
<center>The ideal microscope</center>
 +
| <center>LMF TEAM</center>
 +
 +
|-
 +
| <center>'''DEMO'''</center>
 +
| <center>Optical bench</center>
 +
|
 +
 +
|-
 +
| <center>'''10:30'''</center>
 +
| <center>'''11:00'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>Break</center>
 +
|
 +
 +
|-
 +
| <center>'''11:00'''</center>
 +
 +
 +
 +
| <center>'''12:30'''</center>
 +
| <center>'''LECTURE 1'''</center>
 +
| <center>Basics in Optics</center>
 +
 +
<center>Characteristics of light</center>
 +
 +
<center>Light sources</center>
 +
 +
<center>Reflection, Absorption, Refraction</center>
 +
 +
<center>Diffraction (Demo)</center>
 +
| <center>Humberto</center>
 +
 +
|-
 +
| <center>'''ACTIVITY 1'''</center>
 +
 +
<center>'''(Group Rotation)'''</center>
 +
| # <nowiki>1. [Capillary + oil] + </nowiki>Coin in glass
 +
 +
2. Laser refraction + reflection
 +
 +
# 3. Different objectives – Demo TIRF Objective (immersion solution)
 +
 +
 +
| # 1. Peter
 +
 +
2. Humberto
 +
 +
# 3. Britta
 +
 +
 +
 +
|-
 +
| <center>'''12:30'''</center>
 +
| <center>'''13:30'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>LUNCH</center>
 +
|
 +
 +
|-
 +
| <center>'''13:30'''</center>
 +
| <center>'''15:30'''</center>
 +
| <center>'''LECTURE 2'''</center>
 +
| <center>Introduction to lenses</center>
 +
 +
<center>Lens aberrations</center>
 +
 +
<center>Understanding ray optics</center>
 +
| <center>Humberto</center>
 +
 +
|-
 +
| <center>'''ACTIVITY 2'''</center>
 +
| <center>Ray optics</center>
 +
 +
<center>Drawing ray graphics/diagrams</center>
 +
|
 +
 +
|-
 +
| <center>'''ACTIVITY 3'''</center>
 +
| # 1. Finding focal point of lenses + infinity + Color fringes
 +
# 2. Virtual & real images. 25cm distance
 +
# 3. Image of the arrow in the microscope (upright – inverted)
 +
 +
 +
| # 1. Jan
 +
# 2. Humberto
 +
# 3. Pete + Britta
 +
 +
 +
 +
|-
 +
| <center>'''15:30'''</center>
 +
| <center>'''15:50'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>Break</center>
 +
|
 +
 +
|-
 +
| <center>'''15:50'''</center>
 +
| <center>'''17:00'''</center>
 +
| <center>'''LECTURE 3'''</center>
 +
| <center>Components of the microscope</center>
 +
 +
<center>Focusing, Field of view, Depth of field, depth of focus</center>
 +
 +
<center>Planes (filament DEMO)</center>
 +
 +
<center>Conjugated planes</center>
 +
 +
<center>Koehler illumination</center>
 +
| <center>Humberto</center>
 +
 +
|-
 +
| <center>'''17:00'''</center>
 +
| <center>'''18:30'''</center>
 +
| <center>'''ACTIVITY 4'''</center>
 +
 +
<center>'''Auditorium + Room 036'''</center>
 +
| <center>Glass cube demo and practice - Koehler alignment + Document</center>
 +
| <center>LMF TEAM</center>
 +
 +
|}
 +
 +
 +
 +
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
 +
 +
<center>'''Week 2 '''</center>
 +
 +
 +
{| class="prettytable"
 +
| <center>'''FROM'''</center>
 +
| <center>'''TO'''</center>
 +
| <center>'''ACTIVITY'''</center>
 +
| <center>'''TUESDAY (Dec 2)'''</center>
 +
| <center>'''Responsible person(s)'''</center>
 +
 +
|-
 +
| <center>'''9:00'''</center>
 +
| '''10:00'''
 +
| <center>'''INTRODUCTION'''</center>
 +
 +
 +
 +
| <center>Koehler exercise</center>
 +
 +
<center>Questions</center>
 +
| <center>LMF TEAM</center>
 +
 +
|-
 +
| <center>'''10:00'''</center>
 +
| <center>'''11:00'''</center>
 +
| <center>'''LECTURE 4'''</center>
 +
| <center>Diffraction </center>
 +
 +
Numerical aperture
 +
| <center>Humberto</center>
 +
 +
|-
 +
| <center>'''ACTIVITY 5'''</center>
 +
| # Diffraction grids/gratings
 +
 +
 +
| #
 +
 +
 +
 +
|-
 +
| <center>'''ACTIVITY 6'''</center>
 +
| # Airy disk on the microscope on mirror
 +
 +
 +
| # LMF TEAM
 +
 +
 +
 +
|-
 +
| <center>'''11:00'''</center>
 +
| <center>'''11:15'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>Break</center>
 +
|
 +
 +
|-
 +
| '''11:15'''
 +
| <center>'''11:30'''</center>
 +
| <center>'''LECTURE 5'''</center>
 +
| <center>Apertures (Objective and Condenser) - Cube DEMO</center>
 +
| <center>Humberto</center>
 +
 +
|-
 +
| <center>'''11:30'''</center>
 +
| <center>'''13:00'''</center>
 +
| <center>'''ACTIVITY 7'''</center>
 +
 +
 +
 +
| # Objective cleaning + NA reading + Cover slip thickness measurement
 +
# Diffraction exercise on microscope
 +
# Fluorescent beads with variable NA exercise
 +
 +
 +
| # Pete
 +
# Dan
 +
# Britta
 +
 +
 +
 +
|-
 +
| <center>'''13:00'''</center>
 +
| <center>'''14:00'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>LUNCH</center>
 +
|
 +
 +
|-
 +
| <center>'''14:00'''</center>
 +
| <center>'''15:45'''</center>
 +
| <center>'''LECTURE 6'''</center>
 +
| * <center><nowiki>Contrasting techniques [Dark field - Phase contrast]</nowiki></center>
 +
 +
 +
| Humberto
 +
 +
|-
 +
| <center>'''ACTIVITY 8'''</center>
 +
| Dark field
 +
 +
Phase contrast
 +
| <center>LMF TEAM</center>
 +
 +
|-
 +
| <center>'''15:45'''</center>
 +
| <center>'''16:00'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>Break</center>
 +
|
 +
 +
|-
 +
| <center>'''16:00'''</center>
 +
| <center>'''18:00'''</center>
 +
| <center>'''LECTURE 7'''</center>
 +
| * <center><nowiki>Contrasting techniques [Polarization – DIC]</nowiki></center>
 +
 +
 +
| Humberto
 +
 +
|-
 +
| <center>'''ACTIVITY 9'''</center>
 +
| Polarization – Polarization foils – Crystals
 +
 +
DIC
 +
| <center>LMF TEAM</center>
 +
 +
|}
 +
 +
 +
 +
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
 +
 +
<center>'''Week 2 '''</center>
 +
 +
 +
 +
{| class="prettytable"
 +
| <center>'''FROM'''</center>
 +
| <center>'''TO'''</center>
 +
| <center>'''ACTIVITY'''</center>
 +
| <center>'''WEDNESDAY (Dec 3)'''</center>
 +
| <center>'''Responsible person(s)'''</center>
 +
 +
|-
 +
| <center>'''9:00'''</center>
 +
| '''10:00'''
 +
| <center>'''INTRODUCTION'''</center>
 +
| <center>Questions</center>
 +
| <center>LMF TEAM</center>
 +
 +
|-
 +
| <center>'''10:00'''</center>
 +
| <center>'''11:25'''</center>
 +
| <center>'''LECTURE 8'''</center>
 +
 +
<center>'''(Interactive session)'''</center>
 +
| <center>Objectives</center>
 +
 +
<center>Lens aberrations</center>
 +
 +
<center>Infinity optics </center>
 +
| <center>Humberto</center>
 +
 +
|-
 +
| <center>'''11:25'''</center>
 +
| <center>'''11:15'''</center>
 +
| '''ACTIVITY 10'''
 +
| <center>1. Lens Aberrations</center>
 +
 +
<center>2. Objective reading</center>
 +
 +
<center>3. Infinity optics on optical bench</center>
 +
| # 1. Humberto
 +
 +
2. Britta
 +
 +
3. Jan
 +
 +
|-
 +
| <center>'''11:15'''</center>
 +
| <center>'''11:30'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>Break</center>
 +
|
 +
 +
|-
 +
| <center>'''11:30'''</center>
 +
| <center>'''13:00'''</center>
 +
| <center>'''LECTURE 9'''</center>
 +
| <center>Basics of fluorescence</center>
 +
 +
<center>Fluorochromes</center>
 +
 +
<center>The fluorescence microscope</center>
 +
 +
<center>Filters</center>
 +
| <center>Humberto</center>
 +
 +
<center>Dan</center>
 +
 +
|-
 +
| <center>'''13:00'''</center>
 +
| <center>'''14:00'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>LUNCH</center>
 +
|
 +
 +
|-
 +
| <center>'''14:00'''</center>
 +
| <center>'''15:00'''</center>
 +
| <center>'''LECTURE 11'''</center>
 +
| <center>CCD - DEMO</center>
 +
| Britta
 +
 +
|-
 +
| <center>'''15:00'''</center>
 +
| <center>'''15:15'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>Break</center>
 +
|
 +
 +
|-
 +
| <center>'''15:15'''</center>
 +
| <center>'''16:15'''</center>
 +
| <center>'''LECTURE 12'''</center>
 +
| <center>Digital imaging (live cell imaging – optional)</center>
 +
| Dan
 +
 +
|-
 +
| <center>'''16:15'''</center>
 +
| <center>'''16:45'''</center>
 +
| <center>'''ACTIVITY 11'''</center>
 +
| Mercury lamp exchange and centering
 +
| <center>LMF TEAM</center>
 +
 +
|-
 +
| <center>'''16:45'''</center>
 +
| <center>'''18:30'''</center>
 +
| <center>'''ACTIVITY 12'''</center>
 +
| Wide fields systems + CCD
 +
 +
Fluorescence
 +
 +
 +
 +
| <center>LMF TEAM</center>
 +
 +
|}
 +
 +
 +
 +
<center>'''LIGHT MICROSCOPY - PhD COURSE PLAN'''</center>
 +
 +
<center>'''Week 2 '''</center>
 +
 +
 +
{| class="prettytable"
 +
| <center>'''FROM'''</center>
 +
| <center>'''TO'''</center>
 +
| <center>'''ACTIVITY'''</center>
 +
| <center>'''THURSDAY (Dec 4 )'''</center>
 +
|
 +
 +
|-
 +
| <center>'''9:00'''</center>
 +
| '''9:45'''
 +
| <center>'''INTRODUCTION'''</center>
 +
 +
 +
 +
| <center>Questions</center>
 +
 +
<center>Filter reading</center>
 +
| <center>LMF TEAM</center>
 +
 +
|-
 +
| <center>'''9:45'''</center>
 +
| <center>'''10:30'''</center>
 +
| <center>'''LECTURE 13'''</center>
 +
| <center>Optical sectioning </center>
 +
 +
<center>Confocal Microscopy</center>
 +
 +
<center>2 photon microscopy</center>
 +
 +
<center>Spinning disk</center>
 +
 +
<center>TIRF</center>
 +
| #
 +
#
 +
# Jan
 +
 +
 +
 +
|-
 +
| <center>'''10:30'''</center>
 +
| <center>'''12:00'''</center>
 +
| <center>'''ACTIVITY 13'''</center>
 +
| System rotation ---Confocal-2P- Spinning disk
 +
| Pete – Jan - Britta
 +
 +
|-
 +
| <center>'''12:00'''</center>
 +
| <center>'''13:00'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>LUNCH</center>
 +
|
 +
 +
|-
 +
| <center>'''13:00'''</center>
 +
| <center>'''14:30'''</center>
 +
| <center>'''ACTIVITY 13'''</center>
 +
| System rotation ---Confocal-2P- Spinning disk
 +
| Pete – Jan - Britta
 +
 +
|-
 +
| <center>'''14:30'''</center>
 +
| <center>'''16:00'''</center>
 +
| <center>'''ACTIVITY 13'''</center>
 +
| System rotation ---Confocal-2P- Spinning disk
 +
| <center>Pete – Jan - Britta</center>
 +
 +
|}
 +
 +
 +
 +
 +
{| class="prettytable"
 +
| <center>'''FROM'''</center>
 +
| <center>'''TO'''</center>
 +
| <center>'''ACTIVITY'''</center>
 +
| <center>'''FRIDAY (Dec 5)'''</center>
 +
|
 +
 +
|-
 +
| <center>'''9:00'''</center>
 +
| '''10:00'''
 +
| <center>'''INTRODUCTION'''</center>
 +
| <center>Questions</center>
 +
| <center>LMF TEAM</center>
 +
 +
|-
 +
| <center>'''10:00'''</center>
 +
| '''11:30'''
 +
| <center>'''LECTURE 14'''</center>
 +
| <center>Image processing, Digital images</center>
 +
 +
<center>What is image processing</center>
 +
| <center>Dan</center>
 +
 +
|-
 +
| <center>'''ACTIVITY 14'''</center>
 +
| Quantitative imaging + Image processing
 +
| #
 +
 +
 +
 +
|-
 +
| <center>'''11:30'''</center>
 +
| <center>'''11:45'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>Break</center>
 +
|
 +
 +
|-
 +
| <center>'''11:45'''</center>
 +
| '''13:00'''
 +
| <center>'''LECTURE 14'''</center>
 +
 +
<center>'''(continuation)'''</center>
 +
| <center>Image processing </center>
 +
| Dan
 +
 +
|-
 +
| <center>'''ACTIVITY 14'''</center>
 +
 +
<center>'''(continuation)'''</center>
 +
| Image processing - Image J (FIJI)
 +
|
 +
 +
|-
 +
| <center>'''13:00'''</center>
 +
| <center>'''14:00'''</center>
 +
| <center>'''BREAK'''</center>
 +
| <center>LUNCH</center>
 +
|
 +
 +
|-
 +
| <center>'''14:00'''</center>
 +
| <center>'''15:30'''</center>
 +
|
 +
| <center>Introduction to LMF - Wiki</center>
 +
 +
<center>Final Discussion - Quiz </center>
 +
| <center>LMF TEAM</center>
 +
 +
|}
  
  
  
 
[[category:teaching]]
 
[[category:teaching]]

Revision as of 15:19, 8 October 2009

Peter Evennett's Course Oct 09

Preliminary Schedule

Friday 16th Oct

Setup - all lmf staff move stuff to galleria Equipment needed

  • microscopes
  • optocal bench
  • spinning disk gadget


LIGHT MICROSCOPY - PhD COURSE PLAN
Week 2


FROM
TO
ACTIVITY
MONDAY (Dec 1)
Responsible person(s)
9:00
10:30
INTRODUCTION
Group presentation
Wishes for the course
The ideal microscope
LMF TEAM
DEMO
Optical bench
10:30
11:00
BREAK
Break
11:00
12:30
LECTURE 1
Basics in Optics
Characteristics of light
Light sources
Reflection, Absorption, Refraction
Diffraction (Demo)
Humberto
ACTIVITY 1
(Group Rotation)
 # 1. [Capillary + oil] + Coin in glass

2. Laser refraction + reflection

  1. 3. Different objectives – Demo TIRF Objective (immersion solution)


# 1. Peter

2. Humberto

  1. 3. Britta


12:30
13:30
BREAK
LUNCH
13:30
15:30
LECTURE 2
Introduction to lenses
Lens aberrations
Understanding ray optics
Humberto
ACTIVITY 2
Ray optics
Drawing ray graphics/diagrams
ACTIVITY 3
 # 1. Finding focal point of lenses + infinity + Color fringes
  1. 2. Virtual & real images. 25cm distance
  2. 3. Image of the arrow in the microscope (upright – inverted)


# 1. Jan
  1. 2. Humberto
  2. 3. Pete + Britta


15:30
15:50
BREAK
Break
15:50
17:00
LECTURE 3
Components of the microscope
Focusing, Field of view, Depth of field, depth of focus
Planes (filament DEMO)
Conjugated planes
Koehler illumination
Humberto
17:00
18:30
ACTIVITY 4
Auditorium + Room 036
Glass cube demo and practice - Koehler alignment + Document
LMF TEAM


LIGHT MICROSCOPY - PhD COURSE PLAN
Week 2


FROM
TO
ACTIVITY
TUESDAY (Dec 2)
Responsible person(s)
9:00
 10:00
INTRODUCTION
Koehler exercise
Questions
LMF TEAM
10:00
11:00
LECTURE 4
Diffraction

Numerical aperture

Humberto
ACTIVITY 5
 # Diffraction grids/gratings


#


ACTIVITY 6
 # Airy disk on the microscope on mirror


# LMF TEAM


11:00
11:15
BREAK
Break
11:15
11:30
LECTURE 5
Apertures (Objective and Condenser) - Cube DEMO
Humberto
11:30
13:00
ACTIVITY 7


 # Objective cleaning + NA reading + Cover slip thickness measurement
  1. Diffraction exercise on microscope
  2. Fluorescent beads with variable NA exercise


# Pete
  1. Dan
  2. Britta


13:00
14:00
BREAK
LUNCH
14:00
15:45
LECTURE 6
 *
Contrasting techniques [Dark field - Phase contrast]


 Humberto
ACTIVITY 8
 Dark field

Phase contrast

LMF TEAM
15:45
16:00
BREAK
Break
16:00
18:00
LECTURE 7
 *
Contrasting techniques [Polarization – DIC]


 Humberto
ACTIVITY 9
 Polarization – Polarization foils – Crystals

DIC

LMF TEAM


LIGHT MICROSCOPY - PhD COURSE PLAN
Week 2


FROM
TO
ACTIVITY
WEDNESDAY (Dec 3)
Responsible person(s)
9:00
 10:00
INTRODUCTION
Questions
LMF TEAM
10:00
11:25
LECTURE 8
(Interactive session)
Objectives
Lens aberrations
Infinity optics
Humberto
11:25
11:15
 ACTIVITY 10
1. Lens Aberrations
2. Objective reading
3. Infinity optics on optical bench
 # 1. Humberto

2. Britta

3. Jan

11:15
11:30
BREAK
Break
11:30
13:00
LECTURE 9
Basics of fluorescence
Fluorochromes
The fluorescence microscope
Filters
Humberto
Dan
13:00
14:00
BREAK
LUNCH
14:00
15:00
LECTURE 11
CCD - DEMO
 Britta
15:00
15:15
BREAK
Break
15:15
16:15
LECTURE 12
Digital imaging (live cell imaging – optional)
 Dan
16:15
16:45
ACTIVITY 11
 Mercury lamp exchange and centering
LMF TEAM
16:45
18:30
ACTIVITY 12
 Wide fields systems + CCD

Fluorescence


LMF TEAM


LIGHT MICROSCOPY - PhD COURSE PLAN
Week 2


FROM
TO
ACTIVITY
THURSDAY (Dec 4 )
9:00
 9:45
INTRODUCTION
Questions
Filter reading
LMF TEAM
9:45
10:30
LECTURE 13
Optical sectioning
Confocal Microscopy
2 photon microscopy
Spinning disk
TIRF
 #
  2. Jan


10:30
12:00
ACTIVITY 13
 System rotation ---Confocal-2P- Spinning disk Pete – Jan - Britta
12:00
13:00
BREAK
LUNCH
13:00
14:30
ACTIVITY 13
 System rotation ---Confocal-2P- Spinning disk Pete – Jan - Britta
14:30
16:00
ACTIVITY 13
 System rotation ---Confocal-2P- Spinning disk
Pete – Jan - Britta



FROM
TO
ACTIVITY
FRIDAY (Dec 5)
9:00
 10:00
INTRODUCTION
Questions
LMF TEAM
10:00
 11:30
LECTURE 14
Image processing, Digital images
What is image processing
Dan
ACTIVITY 14
 Quantitative imaging + Image processing #


11:30
11:45
BREAK
Break
11:45
 13:00
LECTURE 14
(continuation)
Image processing
Dan
ACTIVITY 14
(continuation)
 Image processing - Image J (FIJI)
13:00
14:00
BREAK
LUNCH
14:00
15:30
Introduction to LMF - Wiki
Final Discussion - Quiz
LMF TEAM
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