Peter Evennett's Course Oct 09
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==Peter Evennett's Course Oct 09 == | ==Peter Evennett's Course Oct 09 == | ||
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=== Friday 16th Oct === | === Friday 16th Oct === | ||
Setup - all lmf staff move stuff to galleria | Setup - all lmf staff move stuff to galleria | ||
Latest revision as of 15:50, 6 November 2009
Contents |
[edit] Peter Evennett's Course Oct 09
[edit] Friday 16th Oct
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
[edit] DAY ONE (October 19th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:30 | 11:05 | INTRODUCTION | COURSE PRESENTATION GROUP PRESENTATION |
LMF TEAM |
| 11:05 | 11:20 | BREAK | BREAK | BREAK |
| 11:20 | 13:00 | SESSION 1 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Historical aspects of microscopy Introduction to lenses Lens aberrations Magnification Numerical aperture Microscope illumination Conjugate planes Koehler illumination |
PETER EVENNETT LMF TEAM |
| 13:00 | 14:00 | LUNCH | LUNCH | LUNCH |
| 14:00 | 15:30 | SESSION 2 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Demo |
PETER EVENNETT LMF TEAM |
| 15:30 | 15:45 | BREAK | BREAK | BREAK |
| 15:45 | 18:00 | SESSION 3 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY | PETER EVENNETT LMF TEAM |
[edit] DAY TWO (October 20th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:30 | 10:45 | SESSION 1 Theory & Practice |
Resolution Diffraction |
PETER EVENNETT LMF TEAM |
| 10:45 | 11:00 | BREAK | COFFEE BREAK | |
| 11:00 | 13:00 | DEMONSTRATION | Abbe´s diffraction experiments |
PETER EVENNETT LMF TEAM |
| 13:00 | 14:00 | BREAK | LUNCH | |
| 14:00 | 16:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM |
| 16:00 | 16:15 | BREAK | COFFEE BREAK | |
| 18:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM | |
[edit] DAY THREE (October 21th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:30 | 11:30 | SESSION 1 Theory & Practice |
Objective reading Objective cleaning |
PETER EVENNETT LMF TEAM |
| 11:30 | 11:45 | BREAK | BREAK | BREAK |
| 11:45 | 13:00 | SESSION 2 Theory & Practice |
Polarized light microscopy Differential Interference Contrast (DIC) |
PETER EVENNETT LMF TEAM |
| 13:00 | 14:00 | BREAK | LUNCH | |
| 14:00 | 15:15 | SESSION 3 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
| 15:15 | 15:30 | BREAK | BREAK | BREAK |
| 15:30 | 18:00 | SESSION 4 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
[edit] DAY FOUR (October 22th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | DEMO Activities | DEMO materials |
| 9:30 | 10:20 | SESSION 1 Theory |
Question session Fundamentals concepts of fluorescence |
LMF TEAM Dan |
- | - |
| 10:20 | 10:55 | BREAK | BREAK | BREAK | BREAK | BREAK |
| 10:55 | 13:15 | SESSION 2 Theory & Practice |
Fluorescence Microscopy | LMF TEAM Humberto |
• Mercury lamp • Lamp houses • Spectra • Filters • Filter cubes • Fluorescent-labeled samples • Filter spectral charts of each microscope (laminated) |
• Spare lamp houses • Lamps • Ocean optics equipment • Different filters • Four (4) different Filter-sets (cubes), with the appropriate descriptions |
| 13:15 | 14:15 | BREAK | LUNCH | LUNCH | LUNCH | LUNCH |
| 14:15 | 15:45 | SESSION 3 Theory & Practice |
Imaging with CCD | LMF TEAM Britta |
• CCD • Cameras |
• CCD chip • Cameras |
| 15:45 | 16:00 | BREAK | BREAK | |||
| 16:00 | 18:00 | SESSION 4 Practice |
Imaging with CCD cameras using LMF systems | LMF TEAM Jan / Peter / Dan / Britta (*) |
• Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast |
• Microscopes (4) • Sample slides |
[edit] DAY FIVE (October 23th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:30 | 10:20 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
| 10:20 | 10:35 | BREAK | BREAK | BREAK |
| 10:35 | 13:00 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
| 13:00 | 14:00 | BREAK | LUNCH | |
| 14:00 | 15:15 | SESSION 2 Theory & Demo |
Basics of Quantitative Image Analysis (Image Processing) |
LMF TEAM Dan |
| 15:15 | 15:30 | BREAK | BREAK | BREAK |
| 15:30 | 16:30 | SESSION 3 | Course evaluation | LMF TEAM |
[edit] OFFICE DUTIES
[edit] - LMF Members available for users (19th Oct to 23rd Oct) -
| FROM | TO | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
| 9:30 | 12:30 | - | Britta | Peter | Peter | Silke |
| 12:30 | 13:30 | L | U | N | C | H |
| 13:30 | 18:00 | Britta | Dan | Silke | (*)Silke Humberto |
- |
(*) Silke and Humberto will be cleaning and reorganizing the Galeria
