Peter Evennett's Course Oct 09
Preliminary Schedule
Friday 16th Oct
Setup - all lmf staff move stuff to galleria
Equipment needed
- microscopes
- optocal bench
- spinning disk gadget
LIGHT MICROSCOPY - PhD COURSE PLAN
Week 2
| FROM
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TO
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ACTIVITY
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MONDAY (Dec 1)
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Responsible person(s)
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| 9:00
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10:30
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INTRODUCTION
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Group presentation
Wishes for the course
The ideal microscope
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LMF TEAM
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| DEMO
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Optical bench
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|
| 10:30
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11:00
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BREAK
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Break
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| 11:00
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12:30
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LECTURE 1
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Basics in Optics
Characteristics of light
Light sources
Reflection, Absorption, Refraction
Diffraction (Demo)
|
Humberto
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| ACTIVITY 1
(Group Rotation)
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# 1. [Capillary + oil] + Coin in glass
2. Laser refraction + reflection
- 3. Different objectives – Demo TIRF Objective (immersion solution)
|
# 1. Peter
2. Humberto
- 3. Britta
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| 12:30
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13:30
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BREAK
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LUNCH
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| 13:30
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15:30
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LECTURE 2
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Introduction to lenses
Lens aberrations
Understanding ray optics
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Humberto
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| ACTIVITY 2
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Ray optics
Drawing ray graphics/diagrams
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| ACTIVITY 3
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# 1. Finding focal point of lenses + infinity + Color fringes
- 2. Virtual & real images. 25cm distance
- 3. Image of the arrow in the microscope (upright – inverted)
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# 1. Jan
- 2. Humberto
- 3. Pete + Britta
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| 15:30
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15:50
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BREAK
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Break
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|
| 15:50
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17:00
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LECTURE 3
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Components of the microscope
Focusing, Field of view, Depth of field, depth of focus
Planes (filament DEMO)
Conjugated planes
Koehler illumination
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Humberto
|
| 17:00
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18:30
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ACTIVITY 4
Auditorium + Room 036
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Glass cube demo and practice - Koehler alignment + Document
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LMF TEAM
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LIGHT MICROSCOPY - PhD COURSE PLAN
Week 2
| FROM
|
TO
|
ACTIVITY
|
TUESDAY (Dec 2)
|
Responsible person(s)
|
| 9:00
|
10:00
|
INTRODUCTION
|
Koehler exercise
Questions
|
LMF TEAM
|
| 10:00
|
11:00
|
LECTURE 4
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Diffraction
Numerical aperture
|
Humberto
|
| ACTIVITY 5
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# Diffraction grids/gratings
|
#
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| ACTIVITY 6
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# Airy disk on the microscope on mirror
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# LMF TEAM
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| 11:00
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11:15
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BREAK
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Break
|
|
| 11:15
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11:30
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LECTURE 5
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Apertures (Objective and Condenser) - Cube DEMO
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Humberto
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| 11:30
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13:00
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ACTIVITY 7
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# Objective cleaning + NA reading + Cover slip thickness measurement
- Diffraction exercise on microscope
- Fluorescent beads with variable NA exercise
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# Pete
- Dan
- Britta
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| 13:00
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14:00
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BREAK
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LUNCH
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|
| 14:00
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15:45
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LECTURE 6
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* Contrasting techniques [Dark field - Phase contrast]
|
Humberto
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| ACTIVITY 8
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Dark field
Phase contrast
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LMF TEAM
|
| 15:45
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16:00
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BREAK
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Break
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|
| 16:00
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18:00
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LECTURE 7
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* Contrasting techniques [Polarization – DIC]
|
Humberto
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| ACTIVITY 9
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Polarization – Polarization foils – Crystals
DIC
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LMF TEAM
|
LIGHT MICROSCOPY - PhD COURSE PLAN
Week 2
| FROM
|
TO
|
ACTIVITY
|
WEDNESDAY (Dec 3)
|
Responsible person(s)
|
| 9:00
|
10:00
|
INTRODUCTION
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Questions
|
LMF TEAM
|
| 10:00
|
11:25
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LECTURE 8
(Interactive session)
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Objectives
Lens aberrations
Infinity optics
|
Humberto
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| 11:25
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11:15
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ACTIVITY 10
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1. Lens Aberrations
2. Objective reading
3. Infinity optics on optical bench
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# 1. Humberto
2. Britta
3. Jan
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| 11:15
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11:30
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BREAK
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Break
|
|
| 11:30
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13:00
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LECTURE 9
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Basics of fluorescence
Fluorochromes
The fluorescence microscope
Filters
|
Humberto
Dan
|
| 13:00
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14:00
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BREAK
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LUNCH
|
|
| 14:00
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15:00
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LECTURE 11
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CCD - DEMO
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Britta
|
| 15:00
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15:15
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BREAK
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Break
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|
| 15:15
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16:15
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LECTURE 12
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Digital imaging (live cell imaging – optional)
|
Dan
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| 16:15
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16:45
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ACTIVITY 11
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Mercury lamp exchange and centering
|
LMF TEAM
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| 16:45
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18:30
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ACTIVITY 12
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Wide fields systems + CCD
Fluorescence
|
LMF TEAM
|
LIGHT MICROSCOPY - PhD COURSE PLAN
Week 2
| FROM
|
TO
|
ACTIVITY
|
THURSDAY (Dec 4 )
|
|
| 9:00
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9:45
|
INTRODUCTION
|
Questions
Filter reading
|
LMF TEAM
|
| 9:45
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10:30
|
LECTURE 13
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Optical sectioning
Confocal Microscopy
2 photon microscopy
Spinning disk
TIRF
|
#
-
- Jan
|
| 10:30
|
12:00
|
ACTIVITY 13
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System rotation ---Confocal-2P- Spinning disk
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Pete – Jan - Britta
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| 12:00
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13:00
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BREAK
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LUNCH
|
|
| 13:00
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14:30
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ACTIVITY 13
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System rotation ---Confocal-2P- Spinning disk
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Pete – Jan - Britta
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| 14:30
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16:00
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ACTIVITY 13
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System rotation ---Confocal-2P- Spinning disk
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Pete – Jan - Britta
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| FROM
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TO
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ACTIVITY
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FRIDAY (Dec 5)
|
|
| 9:00
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10:00
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INTRODUCTION
|
Questions
|
LMF TEAM
|
| 10:00
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11:30
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LECTURE 14
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Image processing, Digital images
What is image processing
|
Dan
|
| ACTIVITY 14
|
Quantitative imaging + Image processing
|
#
|
| 11:30
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11:45
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BREAK
|
Break
|
|
| 11:45
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13:00
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LECTURE 14
(continuation)
|
Image processing
|
Dan
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| ACTIVITY 14
(continuation)
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Image processing - Image J (FIJI)
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|
| 13:00
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14:00
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BREAK
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LUNCH
|
|
| 14:00
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15:30
|
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Introduction to LMF - Wiki
Final Discussion - Quiz
|
LMF TEAM
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