SD3 - Zeiss Spinning Disc
From BioDIP
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|ill2=Fluorescence (Metal Halide, HXP, 120W) | |ill2=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
− | |ill4=Laser DPSS | + | |ill4=Laser DPSS 445 nm |
|ill5=Laser DPSS 488 nm | |ill5=Laser DPSS 488 nm | ||
− | |ill6=Laser DPSS | + | |ill6=Laser DPSS 520nm |
|ill7=Laser DPSS 561 nm | |ill7=Laser DPSS 561 nm | ||
|ill8=Laser DPSS 639nm | |ill8=Laser DPSS 639nm | ||
Line 37: | Line 37: | ||
Roper evolve 515 EMCCD with manual EM filter holder | Roper evolve 515 EMCCD with manual EM filter holder | ||
− | |reflectors= | + | |reflectors=Pos 1: empty for use with CSU<br> |
− | |features=63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is | + | Pos 2: FS49: Ex 365, BS 395, EM BP445/50 <br> |
− | |software=Zeiss ZEN | + | Pos 3: FS38HE: Ex BP470/40, BS 495, EM BP525/50 <br> |
+ | Pos 4: FS43HE: Ex BP550/25, BS 570, EM BP605/70 <br> | ||
+ | Pos 5: FS50: Ex BP640/30, BS 660, EM BP690/50 <br> | ||
+ | Pos 6: 80/20 mirror for FRAP <br> | ||
+ | |features=63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is 250um and pinhole size is 50um diameter | ||
+ | |software=Zeiss ZEN Blue 2.6 | ||
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD3_-_Zeiss_Spinning_Disc_-_startup_%26_shutdown | |link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD3_-_Zeiss_Spinning_Disc_-_startup_%26_shutdown | ||
|link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedDYzUTM1djl2R2llc1d2ci0xYXRRNGc&output=html | |link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedDYzUTM1djl2R2llc1d2ci0xYXRRNGc&output=html | ||
− | |inv=MOZG04081820000 | + | |inv=MOZG04081820000 |
}} | }} |
Latest revision as of 16:29, 23 March 2020
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
microscope | Zeiss - Axio Observer, Inverted stand, motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage | ||
objectives |
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illumination | |||
detection | Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole radius: 25um, pinhole spacing: 250um;
3 DCs: (1) RTFT 405+488+561; (2) RQFT 405+488+568+647; (3) RTFT 457+514+647 Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50, (2) 458LP, (3) DBP 527/54 + 645/60, (4) 535/30, (5) 629/62, (6) BP 690/50; Roper evolve 515 EMCCD with manual EM filter holder | ||
reflectors | Pos 1: empty for use with CSU Pos 2: FS49: Ex 365, BS 395, EM BP445/50 | ||
features | 63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is 250um and pinhole size is 50um diameter | ||
software | Zeiss ZEN Blue 2.6 | ||
incubation | Cage incubator (T+CO2) | ||
links |
| ||
inv.nr. | MOZG04081820000 |