SD3 - Zeiss Spinning Disc
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{{Equipment | {{Equipment | ||
| − | |system-number=MPI-SD3 | + | |system-number=MPI- SD3 |
|system-name=SD3 - Zeiss Spinning Disc | |system-name=SD3 - Zeiss Spinning Disc | ||
|type=Microscope | |type=Microscope | ||
| Line 7: | Line 7: | ||
|building=MPI-CBG | |building=MPI-CBG | ||
|room=038B | |room=038B | ||
| − | |Facility-logo=MPI-CBG | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| + | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=Fast, "real-time" confocal imaging with DirectFRAP | |description=Fast, "real-time" confocal imaging with DirectFRAP | ||
|applications=fast live confocal imaging | |applications=fast live confocal imaging | ||
| Line 14: | Line 15: | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage | |microscope=motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage | ||
| − | |obj1=Zeiss | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
| − | + | ||
|obj3=Zeiss C-Apochromat 63x 1.2 W | |obj3=Zeiss C-Apochromat 63x 1.2 W | ||
| − | |obj4=Zeiss LCI Plan-Neofluar 63x 1.3 | + | |obj4=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC |
| − | |obj5=Zeiss | + | |obj5=Zeiss Alpha Plan-Apochromat 100x 1.46 Oil DIC |
| + | |obj6=Zeiss Plan-Apochromat 40x 1.3 Oil DIC | ||
| + | |obj7=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC | ||
| + | |obj8=Zeiss LD C-Apochromat 40x 1.1 W | ||
| + | |obj9=Zeiss Plan-Apochromat 63x 1.4 Oil DIC | ||
|ill1=Transmitted Light (LED) | |ill1=Transmitted Light (LED) | ||
|ill2=Fluorescence (Metal Halide, HXP, 120W) | |ill2=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
| − | |ill4=Laser | + | |ill4=Laser DPSS 445 nm |
| − | |ill5=Laser DPSS | + | |ill5=Laser DPSS 488 nm |
| − | |ill6=Laser DPSS 639nm | + | |ill6=Laser DPSS 520nm |
| − | |detection=Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole | + | |ill7=Laser DPSS 561 nm |
| − | Zeiss AxiCam MRm with EM | + | |ill8=Laser DPSS 639nm |
| + | |detection=Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole radius: 25um, pinhole spacing: 250um; | ||
| + | 3 DCs: (1) RTFT 405+488+561; (2) RQFT 405+488+568+647; (3) RTFT 457+514+647 | ||
| + | |||
| + | Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50, (2) 458LP, (3) DBP 527/54 + 645/60, (4) 535/30, (5) 629/62, (6) BP 690/50; | ||
Roper evolve 515 EMCCD with manual EM filter holder | Roper evolve 515 EMCCD with manual EM filter holder | ||
| − | |reflectors= | + | |reflectors=Pos 1: empty for use with CSU<br> |
| − | |features=63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is | + | Pos 2: FS49: Ex 365, BS 395, EM BP445/50 <br> |
| − | |software= | + | Pos 3: FS38HE: Ex BP470/40, BS 495, EM BP525/50 <br> |
| + | Pos 4: FS43HE: Ex BP550/25, BS 570, EM BP605/70 <br> | ||
| + | Pos 5: FS50: Ex BP640/30, BS 660, EM BP690/50 <br> | ||
| + | Pos 6: 80/20 mirror for FRAP <br> | ||
| + | |features=63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is 250um and pinhole size is 50um diameter | ||
| + | |software=Zeiss ZEN Blue 2.6 | ||
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD3_-_Zeiss_Spinning_Disc_-_startup_%26_shutdown | |link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD3_-_Zeiss_Spinning_Disc_-_startup_%26_shutdown | ||
|link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedDYzUTM1djl2R2llc1d2ci0xYXRRNGc&output=html | |link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedDYzUTM1djl2R2llc1d2ci0xYXRRNGc&output=html | ||
| − | |inv=MOZG04081820000 | + | |inv=MOZG04081820000 |
}} | }} | ||
Latest revision as of 16:29, 23 March 2020
|
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Zeiss - Axio Observer, Inverted stand, motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage | ||
| objectives |
| ||
| illumination | |||
| detection | Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole radius: 25um, pinhole spacing: 250um;
3 DCs: (1) RTFT 405+488+561; (2) RQFT 405+488+568+647; (3) RTFT 457+514+647 Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50, (2) 458LP, (3) DBP 527/54 + 645/60, (4) 535/30, (5) 629/62, (6) BP 690/50; Roper evolve 515 EMCCD with manual EM filter holder | ||
| reflectors | Pos 1: empty for use with CSU Pos 2: FS49: Ex 365, BS 395, EM BP445/50 | ||
| features | 63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is 250um and pinhole size is 50um diameter | ||
| software | Zeiss ZEN Blue 2.6 | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | MOZG04081820000 |
