SD3 - Zeiss Spinning Disc
From BioDIP
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− | |Facility-logo=MPI-CBG | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
+ | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=Fast, "real-time" confocal imaging with DirectFRAP | |description=Fast, "real-time" confocal imaging with DirectFRAP | ||
|applications=fast live confocal imaging | |applications=fast live confocal imaging |
Revision as of 12:26, 18 November 2013
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Zeiss - Axio Observer, Inverted stand, motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage | ||
objectives | |||
illumination | |||
detection | Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole radius: 25um, pinhole spacing: 250um;
3 DCs: (1) RTFT 405+488+561; (2) RQFT 405+488+568+647; (3) RTFT 457+514+647 Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50, (2) 458LP, (3) DBP 527/54 + 645/60, (4) 535/30, (5) 629/62, (6) BP 690/50; Roper evolve 515 EMCCD with manual EM filter holder | ||
reflectors | what filters? | ||
features | 63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is ??? nm and back projected pinhole size is ??? nm | ||
software | AxioVision from Zeiss | ||
incubation | Cage incubator (T+CO2) | ||
links |
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inv.nr. | MOZG04081820000 10.1.145.37 |