SD3 - Zeiss Spinning Disc
From BioDIP
(Difference between revisions)
| Line 15: | Line 15: | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage | |microscope=motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage | ||
| − | |obj1=Zeiss | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
|obj2=Zeiss C-Apochromat 40x 1.2 W | |obj2=Zeiss C-Apochromat 40x 1.2 W | ||
|obj3=Zeiss C-Apochromat 63x 1.2 W | |obj3=Zeiss C-Apochromat 63x 1.2 W | ||
Revision as of 10:24, 6 October 2014
|
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Zeiss - Axio Observer, Inverted stand, motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage | ||
| objectives | |||
| illumination | |||
| detection | Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole radius: 25um, pinhole spacing: 250um;
3 DCs: (1) RTFT 405+488+561; (2) RQFT 405+488+568+647; (3) RTFT 457+514+647 Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50, (2) 458LP, (3) DBP 527/54 + 645/60, (4) 535/30, (5) 629/62, (6) BP 690/50; Roper evolve 515 EMCCD with manual EM filter holder | ||
| reflectors | what filters? | ||
| features | 63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is ??? nm and back projected pinhole size is ??? nm | ||
| software | AxioVision from Zeiss | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | MOZG04081820000 10.1.145.37 |
