Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope
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Zeiss - Axio Observer, Inverted stand, motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage
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objectives
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- Zeiss Plan-Apochromat 10x 0.45
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- Zeiss C-Apochromat 63x 1.2 W
- Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
- Zeiss Alpha Plan-Apochromat 100x 1.46 Oil DIC
- Zeiss Plan-Apochromat 40x 1.3 Oil DIC
- Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
- Zeiss LD C-Apochromat 40x 1.1 W
- Zeiss Plan-Apochromat 63x 1.4 Oil DIC
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illumination
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- Transmitted Light (LED)
- Fluorescence (Metal Halide, HXP, 120W)
- Laser Diode 405 nm
- Laser DPSS 445 nm
- Laser DPSS 488 nm
- Laser DPSS 520nm
- Laser DPSS 561 nm
- Laser DPSS 639nm
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detection
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Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole radius: 25um, pinhole spacing: 250um;
3 DCs: (1) RTFT 405+488+561; (2) RQFT 405+488+568+647; (3) RTFT 457+514+647
Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50, (2) 458LP, (3) DBP 527/54 + 645/60, (4) 535/30, (5) 629/62, (6) BP 690/50;
Roper evolve 515 EMCCD with manual EM filter holder
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reflectors
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what filters?
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features
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63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is ??? nm and back projected pinhole size is ??? nm
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software
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Zeiss ZEN 2 Blue
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incubation
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Cage incubator (T+CO2)
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links
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- [ beam path]
- [ advanced system check]
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inv.nr.
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MOZG04081820000 10.1.145.37
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