SD3 - Zeiss Spinning Disc

From BioDIP
(Difference between revisions)
Jump to: navigation, search
Line 30: Line 30:
 
Roper evolve 515 EMCCD with manual EM filter holder
 
Roper evolve 515 EMCCD with manual EM filter holder
 
|reflectors=what filters?
 
|reflectors=what filters?
|features=-
+
|features=63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing  = 204.762 nm. Pinhole spacing is ??? nm and back projected pinhole size is ??? nm
 
|software=AxioVision from Zeiss
 
|software=AxioVision from Zeiss
 
|incubation=Cage incubator (T+CO2)
 
|incubation=Cage incubator (T+CO2)

Revision as of 15:59, 10 November 2011

Suitable for fast live confocal imaging.
MPI-SD3.jpg


Directions

[[image:|150px|text-bottom|right]]

MPI-CBG LMF.jpgMPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axio Observer, Inverted stand, motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage
objectives
Zeiss C-Apochromat 10x 0.45 W
Zeiss C-Apochromat 40x 1.2 W
Zeiss C-Apochromat 63x 1.2 W
Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr
Zeiss alpha Plan-Apochromat 100x 1.46 Oil DIC
illumination
Transmitted Light (LED)
Fluorescence (Metal Halide, HXP, 120W)
Laser Diode 405 nm
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser DPSS 639nm
detection Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole size: 24um, pinhole spacing: 240um

Zeiss AxiCam MRm with EM fitler wheel

Roper evolve 515 EMCCD with manual EM filter holder

reflectors what filters?
features 63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is ??? nm and back projected pinhole size is ??? nm
software AxioVision from Zeiss
incubation Cage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. MOZG04081820000 10.1.145.37



Personal tools