SD3 - Zeiss Spinning Disc

Suitable for fast live confocal imaging.

Directions

MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Zeiss - Axio Observer, Inverted stand, motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage
objectives	Zeiss Plan-Apochromat 10x 0.45 Zeiss C-Apochromat 40x 1.2 W Zeiss C-Apochromat 63x 1.2 W Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr Zeiss alpha Plan-Apochromat 100x 1.46 Oil DIC
illumination	Transmitted Light (LED) Fluorescence (Metal Halide, HXP, 120W) Laser Diode 405 nm Laser Argon Multiline 458, 488, 514 nm Laser DPSS 561 nm Laser DPSS 639nm
detection	Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole radius: 25um, pinhole spacing: 250um; 3 DCs: (1) RTFT 405+488+561; (2) RQFT 405+488+568+647; (3) RTFT 457+514+647 Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50, (2) 458LP, (3) DBP 527/54 + 645/60, (4) 535/30, (5) 629/62, (6) BP 690/50; Roper evolve 515 EMCCD with manual EM filter holder
reflectors	what filters?
features	63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is ??? nm and back projected pinhole size is ??? nm
software	AxioVision from Zeiss
incubation	Cage incubator (T+CO2)
links	manual laser power values [ beam path] [ advanced system check]
inv.nr.	MOZG04081820000 10.1.145.37

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