SD4 - Andor Revolution WD Borealis Mosaic
(change the iQ version to the current one) |
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{{Equipment | {{Equipment | ||
| − | |system-number=MPI-SD4 | + | |system-number=MPI- SD4 |
|system-name=SD4 Andor Revolution WD Borealis Mosaic | |system-name=SD4 Andor Revolution WD Borealis Mosaic | ||
|type=Microscope | |type=Microscope | ||
| Line 11: | Line 11: | ||
|description=Fast, "real-time" spinning disc confocal imaging with photo-activation capability. Fast imaging of large field of view and large tile scans with even illumination. | |description=Fast, "real-time" spinning disc confocal imaging with photo-activation capability. Fast imaging of large field of view and large tile scans with even illumination. | ||
|applications=confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time | |applications=confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time | ||
| − | |image=SD4.jpg | + | |image=SD4 system.jpg |
|stand-name=Olympus - IX83 | |stand-name=Olympus - IX83 | ||
|stand=Inverted | |stand=Inverted | ||
| Line 22: | Line 22: | ||
|obj6=Olympus UAPON 150x 1.45 TIRFM | |obj6=Olympus UAPON 150x 1.45 TIRFM | ||
|ill1=Transmitted Light (LED) | |ill1=Transmitted Light (LED) | ||
| + | |ill2=Fluorescence (LED) | ||
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
| + | |ill4=Laser Diode 445 nm | ||
|ill5=Laser DPSS 488 nm | |ill5=Laser DPSS 488 nm | ||
| + | |ill6=Laser DPSS 514 nm | ||
|ill7=Laser DPSS 561 nm | |ill7=Laser DPSS 561 nm | ||
| − | |ill8=Laser DPSS | + | |ill8=Laser DPSS 638nm |
| + | |ill9=Laser DPSS 726nm | ||
|detection=Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and | |detection=Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and | ||
simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression; Camera 2 (Slave): Andor iXon 888 Ultra | simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression; Camera 2 (Slave): Andor iXon 888 Ultra | ||
| − | |software=Andor iQ version 3. | + | |reflectors=CSU-W scan head:<br> |
| + | 3 main DM: Tripple (for 445nm, 515nm, 640nm laser); Quad (405nm, 488nm, 561nm, 640nm); | ||
| + | Penta (405nm, 488nm, 561nm, 640nm, 720nm)<br> | ||
| + | FW1 (Master): Pos(1) LP568, Pos(2) 525/50, Pos(3) 617/73, Pos(4) 607/36, Pos(5) 685/40, Pos(6) 785/62, Pos(7) pol, Pos(8) LP514, Pos(9) 542/27, Pos(10) 452/45 <br> | ||
| + | FW1 (Slave): Pos(1) 465/30, Pos(2) 541/30, Pos(3) 525/50, Pos(4) 479/40, Pos(5) pol, Pos(6) - Pos(10) empty<br> | ||
| + | |||
| + | reflectors in IX83 stand:<br> | ||
| + | DAPI set (Ex 377/50, DM 409, Em 447/60); GFP set (Ex 472/30, DM 495, Em 520/35); RFP set (Ex 562/40, DM 593, Em 624/40); CY5 set (Ex 628/40, DM 660, Em 692/40); Analyzer; DM for use with Mosaic | ||
| + | |software=Andor iQ version 3.6; MetaMorph version 7.8<br> | ||
| + | communication connections:<br>COM1(Port1): IX83; COM3: CSU-WD; COM4: Mosaic shutter; COM6: prior XY stage; COM7: 16 channel CooLED; <br>Device: Mosaic; Device1: Mosaic laser; DDA06/16 DAC board: Piezo, trigger box; ILE3082M-ILE3082S: ILE master, slave | ||
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
| + | |link1=https://docs.google.com/spreadsheets/d/1UNHFIrXe5ojXuf_fcb3H5PjxFdP4PqSeP5-Yi8-7UaE/pubhtml | ||
}} | }} | ||
Latest revision as of 11:59, 8 June 2020
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[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Olympus - IX83, Inverted stand, motorized with ZDC (hardware autofocus and focus search), Prior NanoScanZ (400um travel range), Prior ProScanIII xy scanning stage | ||
| objectives | |||
| illumination | |||
| detection | Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and
simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression; Camera 2 (Slave): Andor iXon 888 Ultra | ||
| reflectors | CSU-W scan head: 3 main DM: Tripple (for 445nm, 515nm, 640nm laser); Quad (405nm, 488nm, 561nm, 640nm);
Penta (405nm, 488nm, 561nm, 640nm, 720nm) reflectors in IX83 stand: | ||
| features | |||
| software | Andor iQ version 3.6; MetaMorph version 7.8 communication connections: | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
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| inv.nr. |
