SD4 - Andor Revolution WD Borealis Mosaic

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|system-name=SD4 Andor Revolution WD Borealis Mosaic
 
|system-name=SD4 Andor Revolution WD Borealis Mosaic
 
|type=Microscope
 
|type=Microscope
|category=
+
|category=Real-Time Confocal
 
|facility=MPI-CBG LMF
 
|facility=MPI-CBG LMF
 
|building=MPI-CBG
 
|building=MPI-CBG
|room=
+
|room=036
 
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 
|Institute-logo=Logo MPI-CBG.jpg
 
|Institute-logo=Logo MPI-CBG.jpg
|description=
+
|description=Fast, "real-time" spinning disc confocal imaging with photo-activation capability. Fast imaging of large field of view and large tile scans with even illumination.
|applications=
+
|applications=confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time
|image=
+
|image=SD4.jpg
|stand-name=
+
|stand-name=Olympus - IX83
|stand=
+
|stand=Inverted
|incubation=
+
|microscope=motorized with ZDC (hardware autofocus and focus search), Prior NanoScanZ (400um travel range), Prior ProScanIII xy scanning stage
 +
|obj1=Olympus UPlan SApochromat 10x 0.4
 +
|obj2=Olympus UPlanSApo 30x 1,05 S
 +
|obj3=Olympus UPLSAPO 40x 1.25 SIL
 +
|obj4=Olympus UPLSAPO 60x 1.3 SIL
 +
|obj5=Olympus UPlan SApochromat 100x 1.4 Oil
 +
|obj6=Olympus UAPON 150x 1.45 TIRFM
 +
|ill1=Transmitted Light (LED)
 +
|ill3=Laser Diode 405 nm
 +
|ill5=Laser DPSS 488 nm
 +
|ill7=Laser DPSS 561 nm
 +
|ill8=Laser DPSS 639nm
 +
|detection=Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and
 +
simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression; Camera 2 (Slave): Andor iXon 888 Ultra
 +
|software=Andor iQ version 3.3.1; MetaMorph version 7.8
 +
|incubation=Cage incubator (T+CO2)
 
}}
 
}}

Revision as of 18:24, 27 July 2015

Suitable for confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time.
250px


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Olympus - IX83, Inverted stand, motorized with ZDC (hardware autofocus and focus search), Prior NanoScanZ (400um travel range), Prior ProScanIII xy scanning stage
objectives
Olympus UPlan SApochromat 10x 0.4
Olympus UPlanSApo 30x 1,05 S
Olympus UPLSAPO 40x 1.25 SIL
Olympus UPLSAPO 60x 1.3 SIL
Olympus UPlan SApochromat 100x 1.4 Oil
Olympus UAPON 150x 1.45 TIRFM
illumination
Transmitted Light (LED)
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 639nm
detection Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and

simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression; Camera 2 (Slave): Andor iXon 888 Ultra

reflectors
features
software Andor iQ version 3.3.1; MetaMorph version 7.8
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr.



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