SP5 MP, inverse, CRTD
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=BIO17 |
| − | |system-name= | + | |system-name=SP5 MP, inverse, CRTD |
|type=Microscope | |type=Microscope | ||
|category=Multiphoton Laser Scanning | |category=Multiphoton Laser Scanning | ||
| − | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
| − | |building= | + | |building=CRTD |
| − | |room= | + | |room=-1.119 |
| − | | | + | |Facility-logo=Imaging@Biotec.jpg |
| − | | | + | |Institute-logo=CRTD logo.PNG |
| − | |description=Our Leica SP5 MP is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. A | + | |description=Our Leica SP5 MP is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. A Mai Tai IR laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes. A resonating scanner allows fast confocal imaging. |
|applications=- | |applications=- | ||
| − | |image= | + | |image=SP5 MP, inverse, CRTD.jpg |
| − | |stand-name=Leica - | + | |stand-name=Leica - DMI6000 |
|stand=Inverted | |stand=Inverted | ||
| − | |microscope=manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, Multi-Photon laser | + | |microscope=manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional), Multi-Photon laser |
|obj1=Leica HC PL APO 10x 0.4 | |obj1=Leica HC PL APO 10x 0.4 | ||
|obj2=Leica HC PL APO 20x 0.7 Imm Corr | |obj2=Leica HC PL APO 20x 0.7 Imm Corr | ||
| − | |obj3=Leica | + | |obj3=Leica IRAPO L 25x 0.95 W |
| − | + | |obj4=Leica HC PL APO 40x 1.25-0.75 Oil | |
| − | |obj5=Leica | + | |obj5=Leica HC PL APO 63x 1.4-0.6 Oil |
| − | |obj6=Leica | + | |obj6=Leica HC PL APO CS 100x 1.4-0.7 Oil |
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (HBO) | |ill2=Fluorescence (HBO) | ||
| + | |ill3=Laser Diode 405 nm | ||
|ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | |ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | ||
|ill5=Laser DPSS 561 nm | |ill5=Laser DPSS 561 nm | ||
|ill6=Laser HeNe 633 nm | |ill6=Laser HeNe 633 nm | ||
|ill7=Laser Multiphoton 710-990 nm | |ill7=Laser Multiphoton 710-990 nm | ||
| − | |detection=point scanner, | + | |detection=point scanner and resonnant scanner, 2 confocal HyD´s, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole<br>Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70) |
| − | |reflectors=FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 | + | |reflectors=DAPI (A): EX 360/40; BS 400; EM LP 425<br> |
| − | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP. | + | FITC (I3): EX 470/40; BS 510; EM LP 515<br> |
| + | TRITC (N2.1): EX 537/45; BS 580; EM LP 590<br> | ||
| + | Analysator | ||
| + | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP. | ||
|software=- | |software=- | ||
| − | |incubation= | + | |incubation=Cage incubator (T+CO2) |
| − | | | + | |link0=https://ifn.mpi-cbg.de/wiki/ifn/images/f/f2/Startup_and_shutdown-SP5MP.pdf.pdf |
| + | |link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_power_SP5_MP_inverse | ||
|link2=- | |link2=- | ||
| − | |inv= | + | |inv=71928 |
| + | |facility_image=LOGO CRTD.jpg | ||
|objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request | |objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request | ||
|illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe<br>Multi-Photon : Mait Tai (710-990) | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe<br>Multi-Photon : Mait Tai (710-990) | ||
}} | }} | ||
Latest revision as of 15:51, 15 February 2017
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[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Leica - DMI6000, Inverted stand, manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, resonating scanner (optional), Multi-Photon laser | ||
| objectives | |||
| illumination | |||
| detection | point scanner and resonnant scanner, 2 confocal HyD´s, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70) | ||
| reflectors | DAPI (A): EX 360/40; BS 400; EM LP 425 FITC (I3): EX 470/40; BS 510; EM LP 515 | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP. | ||
| software | - | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | 71928 |
