SP5 MP, inverse, CRTD
From BioDIP
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|building=Biotec | |building=Biotec | ||
|room=225 | |room=225 | ||
− | | | + | |Facility-logo=Imaging@Biotec.jpg |
− | | | + | |Institute-logo=LOGO CRTD.jpg |
|description=Our Leica SP5 MP is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. A Mait Tai IR laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes. | |description=Our Leica SP5 MP is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. A Mait Tai IR laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes. | ||
|applications=- | |applications=- | ||
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|link2=- | |link2=- | ||
|inv=- | |inv=- | ||
+ | |facility_image=LOGO CRTD.jpg | ||
|objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request | |objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request | ||
|illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe<br>Multi-Photon : Mait Tai (710-990) | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe<br>Multi-Photon : Mait Tai (710-990) | ||
}} | }} |
Revision as of 17:20, 26 November 2010
Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
microscope | Leica - DMRE, Inverted stand, manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, Multi-Photon laser | ||
objectives | |||
illumination | |||
detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70) | ||
reflectors | FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515 TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP empty empty DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 Analysator | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP. (STED DEMO) | ||
software | - | ||
incubation | Not available | ||
links |
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inv.nr. | - |