SP5 MP, inverse, CRTD

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|building=Biotec
 
|building=Biotec
 
|room=225
 
|room=225
|facility_image=LOGO CRTD.jpg
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|Facility-logo=Imaging@Biotec.jpg
|facility_image=LOGO CRTD.jpg
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|Institute-logo=LOGO CRTD.jpg
 
|description=Our Leica SP5 MP is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. A Mait Tai IR laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes.
 
|description=Our Leica SP5 MP is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. A Mait Tai IR laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes.
 
|applications=-
 
|applications=-
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|link2=-
 
|link2=-
 
|inv=-
 
|inv=-
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|facility_image=LOGO CRTD.jpg
 
|objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request
 
|objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request
 
|illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe<br>Multi-Photon : Mait Tai (710-990)
 
|illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe<br>Multi-Photon : Mait Tai (710-990)
 
}}
 
}}

Revision as of 17:20, 26 November 2010

Suitable for -.
Bioz04.JPG


Directions

Imaging@Biotec.jpg Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. ("Biotec" is not in the list of possible values (BIOTEC, CRTD, DZNE, MTZ, MPI-CBG) for this property., 225)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Leica - DMRE, Inverted stand, manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, Multi-Photon laser
objectives
Leica HC PL APO 10x 0.4
Leica HC PL APO 20x 0.7 Imm Corr
Leica HCX PL APO 40x 1.25-0.75 Oil
Leica HCX PL APO 63x 1.2 W
Leica HCX PL APO 63x 1.4-0.6 Oil
Leica HCX PL APO 100x 1.40-0.70 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
Laser Argon Multiline 458, 477, 488, 496, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Laser Multiphoton 710-990 nm
detection point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole
Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70)
reflectors FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515
TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP
empty
empty
DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425
Analysator
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP. (STED DEMO)
software -
incubation Not available
links
  • [- beam path]
  • [ advanced system check]
inv.nr. -



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