SP5 MP, inverse, CRTD
From BioDIP
(Difference between revisions)
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=04 |
| − | | system-name | + | |system-name=Sp5 MP |
| − | | category | + | |type=Microscope |
| − | | | + | |category=Multiphoton Laser Scanning |
| − | | | + | |facility=Imaging@Biotec |
| − | | | + | |building=Biotec |
| − | | facility_image | + | |room=225 |
| − | + | |facility_image=logo-imagingatbiotec.jpg | |
| − | + | |description=Our Leica SP5 MP is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. A Mait Tai IR laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes. | |
| − | | description | + | |applications=- |
| − | | | + | |image=Bioz04.JPG |
| − | | | + | |stand=Inverted |
| − | | illumination | + | |microscope=manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, Multi-Photon laser |
| − | | detection | + | |obj1=Leica HC PL APO 10x 0.4 |
| − | | reflectors | + | |obj2=Leica HC PL APO 20x 0.7 |
| − | | features | + | |obj3=Leica HCX PL APO 40x 1.25-0.75 Oil |
| − | | incubation | + | |obj4=Leica HCX PL APO 63x 1.2 W |
| − | | link1 | + | |obj5=- |
| − | | link2 | + | |obj6=- |
| + | |obj7=- | ||
| + | |obj8=- | ||
| + | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe<br>Multi-Photon : Mait Tai (710-990) | ||
| + | |detection=point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole<br>Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70) | ||
| + | |reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP | ||
| + | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
| + | |incubation=- | ||
| + | |link1=[http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsSHvxFOx1NJA LPM] | ||
| + | |link2=- | ||
| + | |inv=0 | ||
| + | |objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request | ||
}} | }} | ||
Revision as of 13:57, 4 September 2009
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | , Inverted stand, manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, Multi-Photon laser | ||
| objectives | |||
| illumination |
| ||
| detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70) | ||
| reflectors | DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515 TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
| software | |||
| incubation | - | ||
| links |
| ||
| inv.nr. | 0 |
