D1 - SPIM Demo
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(Redirected from SPIM Demo)
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Zeiss prototype SPIM 3D stand, 3D stand, imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | ||
objectives | |||
illumination | |||
detection | Zeiss CCD Axiocam camera. Max 3fps full chip. Faster with binning. | ||
reflectors | 50/50 for sample location/lightsheet alignment etc.,
BrightLine HC FITC F36-501, Green Fset09, BrightLine HC mCherry 624/40 F37-624 emitter only, YFP FSet46he, CFP FSet51, BrightLine HC Green 514/30, | ||
features | Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium.
It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry."Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium. It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry." cannot be used as a page name in this wiki. | ||
software | Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
incubation | Stage incubator (T) | ||
links |
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inv.nr. | 0 10.1.235.28 |