Ultramicroscope, CRTD

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Line 9: Line 9:
 
|Facility-logo=Imaging@Biotec.jpg
 
|Facility-logo=Imaging@Biotec.jpg
 
|Institute-logo=CRTD logo.PNG
 
|Institute-logo=CRTD logo.PNG
|description=Static light sheet microscope with overlapping light sheets from different angles to reduce shadowing.
+
|description=Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/emission filter wheel a broad range of fluorophores can be used.
|applications=3D Imaging of large specimen
+
|applications=3D multicolor imaging of large transparent specimen.
 
|image=Ultramicroscope, CRTD.jpg
 
|image=Ultramicroscope, CRTD.jpg
 
|stand-name=Olympus - MVX10
 
|stand-name=Olympus - MVX10
 
|stand=Upright
 
|stand=Upright
|microscope=motorized xyz glass cuvette including sample holder and dipping cap for objectives
+
|microscope=motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available
 
|obj1=Olympus MVPLAPO 2x 0.5
 
|obj1=Olympus MVPLAPO 2x 0.5
 
|ill1=Laser Fianium Supercontinuum SC400-4
 
|ill1=Laser Fianium Supercontinuum SC400-4
Line 21: Line 21:
 
Pixel size 6.5 x 6.5 μm2
 
Pixel size 6.5 x 6.5 μm2
 
|reflectors=7 excitation and emission filter:<br>
 
|reflectors=7 excitation and emission filter:<br>
DAPI<br>
+
'''DAPI:''' ex. 405/40 ET, em. 460/50 ET Bandpass<br>
405/40 ET Bandpass<br>
+
460/50 ET Bandpass<br>
+
 
GFP<br>
 
GFP<br>
 
470/40 ET Bandpass<br>
 
470/40 ET Bandpass<br>

Revision as of 10:25, 5 May 2014

Suitable for 3D multicolor imaging of large transparent specimen..
250px


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.120)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Olympus - MVX10, Upright stand, motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available
objectives
Olympus MVPLAPO 2x 0.5
illumination
Laser Fianium Supercontinuum SC400-4
detection Andor Neo sCMOS camera

Pixel 2560 x 2160
Pixel size 6.5 x 6.5 μm2

reflectors 7 excitation and emission filter:

DAPI: ex. 405/40 ET, em. 460/50 ET Bandpass
GFP
470/40 ET Bandpass
525/50 ET Bandpass
YFP
500/20 ET Bandpass
535/30 ET Bandpass
Cy3. DsRed, TRITC
545/30 ET Bandpass
610/60 ET Bandpass
mCherry, TxRed
560/40 ET Bandpass
630/75 ET Bandpass
Alexa 610, Alexa 633, E2-Crimson
605/50 ET Bandpass
670/50 ET Bandpass
Cy7, Alexa 750, DyLight 750
710/75 ET Bandpass
810/90 ET Bandpass

features -
software LaVision BioTec ImSpector Software

Windows 7"LaVision BioTec ImSpector Software<br /> Windows 7" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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