Ultramicroscope, CRTD

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|reflectors=7 excitation and emission filter:<br>
 
|reflectors=7 excitation and emission filter:<br>
 
'''DAPI:''' ex. 405/40 ET, em. 460/50 ET Bandpass<br>
 
'''DAPI:''' ex. 405/40 ET, em. 460/50 ET Bandpass<br>
GFP<br>
+
'''GFP:'''  ex. 470/40 ET, em. 525/50 ET Bandpass<br>
470/40 ET Bandpass<br>
+
'''YFP:'''  ex. 500/20 ET, em. 535/30 ET Bandpass<br>
525/50 ET Bandpass<br>
+
'''Cy3/DsRed/TRITC:''' ex. 545/30, em. 610/60 ET Bandpass<br>
YFP<br>
+
'''mCherry/ TxRed:''' ex. 560/40, em. 630/75 ET Bandpass<br>
500/20 ET Bandpass<br>
+
'''Alexa 610/Alexa 633/E2-Crimson:''' ex. 605/50, em. 670/50 ET Bandpass<br>
535/30 ET Bandpass<br>
+
'''Cy7/Alexa 750/DyLight 750:''' ex. 710/75, em. 810/90 ET Bandpass
Cy3. DsRed, TRITC<br>
+
545/30 ET Bandpass<br>
+
610/60 ET Bandpass<br>
+
mCherry, TxRed<br>
+
560/40 ET Bandpass<br>
+
630/75 ET Bandpass<br>
+
Alexa 610, Alexa 633, E2-Crimson<br>
+
605/50 ET Bandpass<br>
+
670/50 ET Bandpass<br>
+
Cy7, Alexa 750, DyLight 750<br>
+
710/75 ET Bandpass<br>
+
810/90 ET Bandpass
+
 
|features=-
 
|features=-
 
|software=LaVision BioTec ImSpector Software<br>
 
|software=LaVision BioTec ImSpector Software<br>

Revision as of 10:29, 5 May 2014

Suitable for 3D multicolor imaging of large transparent specimen..
250px


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.120)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Olympus - MVX10, Upright stand, motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available
objectives
Olympus MVPLAPO 2x 0.5
illumination
Laser Fianium Supercontinuum SC400-4
detection Andor Neo sCMOS camera

Pixel 2560 x 2160
Pixel size 6.5 x 6.5 μm2

reflectors 7 excitation and emission filter:

DAPI: ex. 405/40 ET, em. 460/50 ET Bandpass
GFP: ex. 470/40 ET, em. 525/50 ET Bandpass
YFP: ex. 500/20 ET, em. 535/30 ET Bandpass
Cy3/DsRed/TRITC: ex. 545/30, em. 610/60 ET Bandpass
mCherry/ TxRed: ex. 560/40, em. 630/75 ET Bandpass
Alexa 610/Alexa 633/E2-Crimson: ex. 605/50, em. 670/50 ET Bandpass
Cy7/Alexa 750/DyLight 750: ex. 710/75, em. 810/90 ET Bandpass

features -
software LaVision BioTec ImSpector Software

Windows 7"LaVision BioTec ImSpector Software<br /> Windows 7" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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