Ultramicroscope, CRTD

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'''Alexa 610/Alexa 633/E2-Crimson:''' ex. 605/50, em. 670/50 ET Bandpass<br>
 
'''Alexa 610/Alexa 633/E2-Crimson:''' ex. 605/50, em. 670/50 ET Bandpass<br>
 
'''Cy7/Alexa 750/DyLight 750:''' ex. 710/75, em. 810/90 ET Bandpass
 
'''Cy7/Alexa 750/DyLight 750:''' ex. 710/75, em. 810/90 ET Bandpass
|features=-
+
|features=Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples.
 
|software=LaVision BioTec ImSpector Software<br>
 
|software=LaVision BioTec ImSpector Software<br>
 
Windows 7
 
Windows 7

Revision as of 10:30, 5 May 2014

Suitable for 3D multicolor imaging of large transparent specimen..
250px


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.120)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Olympus - MVX10, Upright stand, motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available
objectives
Olympus MVPLAPO 2x 0.5
illumination
Laser Fianium Supercontinuum SC400-4
detection Andor Neo sCMOS camera

Pixel 2560 x 2160
Pixel size 6.5 x 6.5 μm2

reflectors 7 excitation and emission filter:

DAPI: ex. 405/40 ET, em. 460/50 ET Bandpass
GFP: ex. 470/40 ET, em. 525/50 ET Bandpass
YFP: ex. 500/20 ET, em. 535/30 ET Bandpass
Cy3/DsRed/TRITC: ex. 545/30, em. 610/60 ET Bandpass
mCherry/ TxRed: ex. 560/40, em. 630/75 ET Bandpass
Alexa 610/Alexa 633/E2-Crimson: ex. 605/50, em. 670/50 ET Bandpass
Cy7/Alexa 750/DyLight 750: ex. 710/75, em. 810/90 ET Bandpass

features Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples.
software LaVision BioTec ImSpector Software

Windows 7"LaVision BioTec ImSpector Software<br /> Windows 7" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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