Ultramicroscope, CRTD
From BioDIP
(Difference between revisions)
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|description=Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/emission filter wheel a broad range of fluorophores can be used. | |description=Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/emission filter wheel a broad range of fluorophores can be used. | ||
|applications=3D multicolor imaging of large transparent specimen. | |applications=3D multicolor imaging of large transparent specimen. | ||
− | |image= | + | |image=Ultramic.jpg |
|stand-name=Olympus - MVX10 | |stand-name=Olympus - MVX10 | ||
|stand=Upright | |stand=Upright | ||
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'''Alexa 610/Alexa 633/E2-Crimson:''' ex. 605/50, em. 670/50 ET Bandpass<br> | '''Alexa 610/Alexa 633/E2-Crimson:''' ex. 605/50, em. 670/50 ET Bandpass<br> | ||
'''Cy7/Alexa 750/DyLight 750:''' ex. 710/75, em. 810/90 ET Bandpass | '''Cy7/Alexa 750/DyLight 750:''' ex. 710/75, em. 810/90 ET Bandpass | ||
− | |features=Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a color correction lens in place in front of the sCMOS camera to correct in z for different wavelength. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions. | + | |features=Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a color correction lens in place in front of the sCMOS camera to correct in z for different wavelength. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions. |
|software=LaVision BioTec ImSpector Software<br> | |software=LaVision BioTec ImSpector Software<br> | ||
Windows 7 | Windows 7 |
Revision as of 10:51, 6 May 2014
Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
microscope | Olympus - MVX10, Upright stand, motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available | ||
objectives | |||
illumination | |||
detection | Andor Neo sCMOS camera Pixel 2560 x 2160 | ||
reflectors | 7 excitation and emission filter: DAPI: ex. 405/40 ET, em. 460/50 ET Bandpass | ||
features | Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a color correction lens in place in front of the sCMOS camera to correct in z for different wavelength. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions."Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a color correction lens in place in front of the sCMOS camera to correct in z for different wavelength. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions." cannot be used as a page name in this wiki. | ||
software | LaVision BioTec ImSpector Software Windows 7"LaVision BioTec ImSpector Software<br /> Windows 7" cannot be used as a page name in this wiki. | ||
incubation | Not available | ||
links |
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inv.nr. | 0 |