Ultramicroscope, CRTD

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|Facility-logo=Imaging@Biotec.jpg
 
|Facility-logo=Imaging@Biotec.jpg
 
|Institute-logo=CRTD logo.PNG
 
|Institute-logo=CRTD logo.PNG
|description=Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/emission filter wheel a broad range of fluorophores can be used.
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|description=Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm^3 in xyz. Due to the white light laser/emission filter wheel a broad range of fluorophores can be used.
 
|applications=3D multicolor imaging of large transparent specimen.
 
|applications=3D multicolor imaging of large transparent specimen.
 
|image=Ultramic.jpg
 
|image=Ultramic.jpg

Revision as of 15:36, 6 May 2014

Suitable for 3D multicolor imaging of large transparent specimen..
Ultramic.jpg


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.120)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Olympus - MVX10, Upright stand, motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available
objectives
Olympus MVPLAPO 2x 0.5
illumination
Laser Fianium Supercontinuum SC400-4
detection Andor Neo sCMOS camera

Pixel 2560 x 2160
Pixel size 6.5 x 6.5 μm2

reflectors 7 excitation and emission filter:

DAPI: ex. 405/40 ET, em. 460/50 ET Bandpass
GFP: ex. 470/40 ET, em. 525/50 ET Bandpass
YFP: ex. 500/20 ET, em. 535/30 ET Bandpass
Cy3/DsRed/TRITC: ex. 545/30, em. 610/60 ET Bandpass
mCherry/ TxRed: ex. 560/40, em. 630/75 ET Bandpass
Alexa 610/Alexa 633/E2-Crimson: ex. 605/50, em. 670/50 ET Bandpass
Cy7/Alexa 750/DyLight 750: ex. 710/75, em. 810/90 ET Bandpass

features Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a color correction lens in place in front of the sCMOS camera to correct in z for different wavelength. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions."Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a color correction lens in place in front of the sCMOS camera to correct in z for different wavelength. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions." cannot be used as a page name in this wiki.
software LaVision BioTec ImSpector Software

Windows 7"LaVision BioTec ImSpector Software<br /> Windows 7" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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