Ultramicroscope, CRTD
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|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
|Institute-logo=CRTD logo.PNG | |Institute-logo=CRTD logo.PNG | ||
| − | |description=Static light sheet microscope with | + | |description=Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm<sup>3</sup> in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used. |
| − | |applications=3D | + | |applications=3D multicolor imaging of large transparent specimen |
| − | |image= | + | |image=Ultramic.jpg |
| − | |stand-name=Olympus | + | |stand-name=Olympus/MVX10 |
| − | |stand= | + | |stand=upright |
| − | |microscope=motorized xyz glass cuvette | + | |microscope=motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available |
| − | |obj1=Olympus MVPLAPO 2x 0.5 | + | |obj1=Olympus MVPLAPO 2x/0.5 |
|ill1=Laser Fianium Supercontinuum SC400-4 | |ill1=Laser Fianium Supercontinuum SC400-4 | ||
|detection=Andor Neo sCMOS camera<br> | |detection=Andor Neo sCMOS camera<br> | ||
Pixel 2560 x 2160<br> | Pixel 2560 x 2160<br> | ||
| − | Pixel size 6.5 x 6.5 | + | Pixel size 6.5 x 6.5 μm<sup>2</sup> |
|reflectors=7 excitation and emission filter:<br> | |reflectors=7 excitation and emission filter:<br> | ||
| − | DAPI | + | '''DAPI:''' ex. 405/40 ET, em. 460/50 ET Bandpass<br> |
| − | 405/40 ET | + | '''GFP:''' ex. 470/40 ET, em. 525/50 ET Bandpass<br> |
| − | 460/50 ET Bandpass<br> | + | '''YFP:''' ex. 500/20 ET, em. 535/30 ET Bandpass<br> |
| − | GFP | + | '''Cy3/DsRed/TRITC:''' ex. 545/30, em. 610/60 ET Bandpass<br> |
| − | 470/40 ET | + | '''mCherry/ TxRed:''' ex. 560/40, em. 630/75 ET Bandpass<br> |
| − | 525/50 ET Bandpass<br> | + | '''Alexa 610/Alexa 633/E2-Crimson:''' ex. 605/50, em. 670/50 ET Bandpass<br> |
| − | YFP | + | '''Cy7/Alexa 750/DyLight 750:''' ex. 710/75, em. 810/90 ET Bandpass |
| − | 500/20 ET | + | |features=Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions. |
| − | 535/30 ET Bandpass<br> | + | |
| − | Cy3 | + | |
| − | 545/30 | + | |
| − | 610/60 ET Bandpass<br> | + | |
| − | mCherry | + | |
| − | 560/40 | + | |
| − | 630/75 ET Bandpass<br> | + | |
| − | Alexa 610 | + | |
| − | 605/50 | + | |
| − | 670/50 ET Bandpass<br> | + | |
| − | Cy7 | + | |
| − | 710/75 | + | |
| − | 810/90 ET Bandpass | + | |
| − | |features= | + | |
|software=LaVision BioTec ImSpector Software<br> | |software=LaVision BioTec ImSpector Software<br> | ||
| − | Windows 7 | + | Windows 7, 64 bit |
|incubation=Not available | |incubation=Not available | ||
|inv=0 | |inv=0 | ||
}} | }} | ||
Latest revision as of 15:49, 6 May 2014
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[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Olympus/MVX10, upright stand, motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available | ||
| objectives | |||
| illumination | |||
| detection | Andor Neo sCMOS camera Pixel 2560 x 2160 | ||
| reflectors | 7 excitation and emission filter: DAPI: ex. 405/40 ET, em. 460/50 ET Bandpass | ||
| features | Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions."Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions." cannot be used as a page name in this wiki. | ||
| software | LaVision BioTec ImSpector Software Windows 7, 64 bit"LaVision BioTec ImSpector Software<br /> Windows 7, 64 bit" cannot be used as a page name in this wiki. | ||
| incubation | Not available | ||
| links |
| ||
| inv.nr. | 0 |
