Ultramicroscope, CRTD
From BioDIP
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|Institute-logo=CRTD logo.PNG | |Institute-logo=CRTD logo.PNG | ||
|description=Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm<sup>3</sup> in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used. | |description=Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm<sup>3</sup> in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used. | ||
| − | |applications=3D multicolor imaging of large transparent specimen | + | |applications=3D multicolor imaging of large transparent specimen |
|image=Ultramic.jpg | |image=Ultramic.jpg | ||
|stand-name=Olympus/MVX10 | |stand-name=Olympus/MVX10 | ||
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|detection=Andor Neo sCMOS camera<br> | |detection=Andor Neo sCMOS camera<br> | ||
Pixel 2560 x 2160<br> | Pixel 2560 x 2160<br> | ||
| − | Pixel size 6.5 x 6.5 | + | Pixel size 6.5 x 6.5 μm<sup>2</sup> |
|reflectors=7 excitation and emission filter:<br> | |reflectors=7 excitation and emission filter:<br> | ||
'''DAPI:''' ex. 405/40 ET, em. 460/50 ET Bandpass<br> | '''DAPI:''' ex. 405/40 ET, em. 460/50 ET Bandpass<br> | ||
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|features=Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions. | |features=Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions. | ||
|software=LaVision BioTec ImSpector Software<br> | |software=LaVision BioTec ImSpector Software<br> | ||
| − | Windows 7 | + | Windows 7, 64 bit |
|incubation=Not available | |incubation=Not available | ||
|inv=0 | |inv=0 | ||
}} | }} | ||
Latest revision as of 15:49, 6 May 2014
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[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Olympus/MVX10, upright stand, motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available | ||
| objectives | |||
| illumination | |||
| detection | Andor Neo sCMOS camera Pixel 2560 x 2160 | ||
| reflectors | 7 excitation and emission filter: DAPI: ex. 405/40 ET, em. 460/50 ET Bandpass | ||
| features | Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions."Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions." cannot be used as a page name in this wiki. | ||
| software | LaVision BioTec ImSpector Software Windows 7, 64 bit"LaVision BioTec ImSpector Software<br /> Windows 7, 64 bit" cannot be used as a page name in this wiki. | ||
| incubation | Not available | ||
| links |
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| inv.nr. | 0 |
