W1 - Olympus TIRF
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=MPI- W1 |
| − | | system-name | + | |system-name=Olympus TIRF |
| − | | category | + | |type=Microscope |
| − | | | + | |category=Total Internal Reflection |
| − | | | + | |facility=MPI-CBG LMF |
| − | + | |building=MPI-CBG | |
| − | | | + | |room=U32 |
| − | | | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| − | + | |Institute-logo=Logo MPI-CBG.jpg | |
| − | | description | + | |description=Olympus TIRF setup. |
| − | | applications | + | |applications=membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics |
| − | | | + | |image=MPI_W1_TIRF.jpg |
| − | | | + | |stand-name=Olympus - IX71 |
| − | | | + | |stand=Inverted |
| − | | detection | + | |microscope=manual, manual XY stage |
| − | | reflectors | + | |obj1=Olympus UPlanSApochromat 10x 0.4 |
| − | | features | + | |obj2=Olympus MPlanFluarN 100x 0.9 |
| − | | incubation | + | |obj3=Olympus PlanApochromat 100x 1.45 Oil TIRFM |
| + | |obj4=Olympus UApochromat 150x 1.45 Oil TIRFM | ||
| + | |obj5=Olympus Apo N 100x 1.70 HIOil TIRFM | ||
| + | |ill1=Transmitted Light (Halogen) | ||
| + | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) | ||
| + | |ill3=Laser DPSS 491 nm | ||
| + | |ill4=Laser DPSS 561 nm | ||
| + | |detection=Andor iXon Ultra 897 BV back illuminated EMCCD [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:Andor_iXon_Ultra_897_Specifications.pdf spec sheet];<br> pixel size of EMCCD chip: 16um; | ||
| + | image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar); | ||
| + | with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar) | ||
| + | |reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF<br>microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| + | |features=custom-built TIRF condenser, manual TIRF angle adjustment | ||
| + | |software=iQ 2.9 + iQ 3.2 [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>with Python IDE | ||
| + | |incubation=Stage incubator (T+CO2) + objective heater | ||
| + | |link1=https://docs.google.com/spreadsheets/d/1iG4mhQX-ejdwQwYcXd7k2OapcL1n7seb5BWR02L6bwg/edit?usp=sharing | ||
| + | |inv=404504 10.1.145.13 <br> AIM 2601000564 | ||
}} | }} | ||
Latest revision as of 18:22, 10 December 2018
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[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Olympus - IX71, Inverted stand, manual, manual XY stage | ||
| objectives | |||
| illumination | |||
| detection | [[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet; pixel size of EMCCD chip: 16um; image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar); with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar)]] | ||
| reflectors | Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | [[software::iQ 2.9 + iQ 3.2 iQ3 user guide with Python IDE]] | ||
| incubation | Stage incubator (T+CO2) + objective heater | ||
| links |
| ||
| inv.nr. | 404504 10.1.145.13 AIM 2601000564"404504 10.1.145.13 <br /> AIM 2601000564" cannot be used as a page name in this wiki. |
