W1 - Olympus TIRF

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{{Equipment
 
{{Equipment
−
|system-number=MPI06
+
|system-number=MPI- W1
 
|system-name=Olympus TIRF
 
|system-name=Olympus TIRF
 
|type=Microscope
 
|type=Microscope
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|facility=MPI-CBG LMF
 
|facility=MPI-CBG LMF
 
|building=MPI-CBG
 
|building=MPI-CBG
−
|room=038B
+
|room=U32
−
|facility_image=logo-lmf.jpg
+
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 +
|Institute-logo=Logo MPI-CBG.jpg
 
|description=Olympus TIRF setup.
 
|description=Olympus TIRF setup.
−
|applications=membrane trafficking studies
+
|applications=membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics
−
|image=MPI06.jpg
+
|image=MPI_W1_TIRF.jpg
 +
|stand-name=Olympus - IX71
 
|stand=Inverted
 
|stand=Inverted
−
|microscope=manual IX71, manual XY stage
+
|microscope=manual, manual XY stage
−
|objectives=PlanApo 100x / 1.45 Oil TIRFM
+
|obj1=Olympus UPlanSApochromat 10x 0.4
−
|illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt
+
|obj2=Olympus MPlanFluarN 100x 0.9
−
|detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
+
|obj3=Olympus PlanApochromat 100x 1.45 Oil TIRFM
−
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um;
+
|obj4=Olympus UApochromat 150x 1.45 Oil TIRFM
−
|reflectors=Cairn optosplit II image splitter:<br>two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em<br>Sutter filter wheel: <br>position 0: BL HC 520/15, 1: BL HC 628/40,  2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open,  6: dual emitter:  Z491/561,  7: blocked,  8: BL HC LP488,  9: BL HC 617/73<br> microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em,  2: for TIRF Z488Ex/DCZDC488/BP 520/40,  3: empty, 4: RFP cube,  5: dual Ex and DC for 491 and 561 laser excitation, no emitter!,  6: only DC and EM LP 575
+
|obj5=Olympus Apo N 100x 1.70 HIOil TIRFM
 +
|ill1=Transmitted Light (Halogen)
 +
|ill2=Fluorescence (Metal Halide, X-Cite, 120W)
 +
|ill3=Laser DPSS 491 nm
 +
|ill4=Laser DPSS 561 nm
 +
|detection=Andor iXon Ultra 897 BV back illuminated EMCCD [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:Andor_iXon_Ultra_897_Specifications.pdf spec sheet];<br> pixel size of EMCCD chip: 16um;
 +
image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar);
 +
with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar)
 +
|reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF<br>microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em,  2: for TIRF Z488Ex/DCZDC488/BP 520/40,  3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!,  5: dual Ex and DC for 491 and 561 laser excitation, no emitter!,  6: only DC and EM LP 575
 
|features=custom-built TIRF condenser, manual TIRF angle adjustment
 
|features=custom-built TIRF condenser, manual TIRF angle adjustment
−
|incubation=stage incubator (T+CO2) + objective heater
+
|software=iQ 2.9 + iQ 3.2 [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>with Python IDE
−
|link1=[http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA laser power]
+
|incubation=Stage incubator (T+CO2) + objective heater
−
|link2=-
+
|link1=https://docs.google.com/spreadsheets/d/1iG4mhQX-ejdwQwYcXd7k2OapcL1n7seb5BWR02L6bwg/edit?usp=sharing
−
|inv=404504
+
|inv=404504   10.1.145.13    <br> AIM 2601000564
−
|obj1=Empty position
+
−
|obj2=Empty position
+
−
|obj3=Empty position
+
−
|obj4=Empty position
+
−
|obj5=Empty position
+
−
|obj6=Empty position
+
−
|obj7=Empty position
+
−
|obj8=Empty position
+
 
}}
 
}}

Latest revision as of 18:22, 10 December 2018

Suitable for membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics.
MPI W1 TIRF.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, U32)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Olympus - IX71, Inverted stand, manual, manual XY stage
objectives
Olympus UPlanSApochromat 10x 0.4
Olympus MPlanFluarN 100x 0.9
Olympus PlanApochromat 100x 1.45 Oil TIRFM
Olympus UApochromat 150x 1.45 Oil TIRFM
Olympus Apo N 100x 1.70 HIOil TIRFM
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
Laser DPSS 491 nm
Laser DPSS 561 nm
detection [[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet;
pixel size of EMCCD chip: 16um;

image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar); with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar)]]

reflectors Hamamatsu W-View image splitter:
two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF
microscope filter turret:
position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575
features custom-built TIRF condenser, manual TIRF angle adjustment
software [[software::iQ 2.9 + iQ 3.2 iQ3 user guide
with Python IDE]]
incubation Stage incubator (T+CO2) + objective heater
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 404504 10.1.145.13
AIM 2601000564"404504 10.1.145.13 <br /> AIM 2601000564" cannot be used as a page name in this wiki.


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