W1 - Olympus TIRF
From BioDIP
(Difference between revisions)
(update LPM link) |
|||
| (6 intermediate revisions by 2 users not shown) | |||
| Line 16: | Line 16: | ||
|microscope=manual, manual XY stage | |microscope=manual, manual XY stage | ||
|obj1=Olympus UPlanSApochromat 10x 0.4 | |obj1=Olympus UPlanSApochromat 10x 0.4 | ||
| − | |obj2=Olympus PlanApochromat 100x 1.45 TIRFM | + | |obj2=Olympus MPlanFluarN 100x 0.9 |
| − | | | + | |obj3=Olympus PlanApochromat 100x 1.45 Oil TIRFM |
| + | |obj4=Olympus UApochromat 150x 1.45 Oil TIRFM | ||
| + | |obj5=Olympus Apo N 100x 1.70 HIOil TIRFM | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (Metal Halide, X-Cite, 120W) | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) | ||
| Line 27: | Line 29: | ||
|reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF<br>microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | |reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF<br>microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
|features=custom-built TIRF condenser, manual TIRF angle adjustment | |features=custom-built TIRF condenser, manual TIRF angle adjustment | ||
| − | |software=iQ 2.9 + iQ 3. | + | |software=iQ 2.9 + iQ 3.2 [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>with Python IDE |
|incubation=Stage incubator (T+CO2) + objective heater | |incubation=Stage incubator (T+CO2) + objective heater | ||
| − | |link1= | + | |link1=https://docs.google.com/spreadsheets/d/1iG4mhQX-ejdwQwYcXd7k2OapcL1n7seb5BWR02L6bwg/edit?usp=sharing |
|inv=404504 10.1.145.13 <br> AIM 2601000564 | |inv=404504 10.1.145.13 <br> AIM 2601000564 | ||
| − | |||
| − | |||
| − | |||
}} | }} | ||
Latest revision as of 18:22, 10 December 2018
|
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Olympus - IX71, Inverted stand, manual, manual XY stage | ||
| objectives | |||
| illumination | |||
| detection | [[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet; pixel size of EMCCD chip: 16um; image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar); with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar)]] | ||
| reflectors | Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | [[software::iQ 2.9 + iQ 3.2 iQ3 user guide with Python IDE]] | ||
| incubation | Stage incubator (T+CO2) + objective heater | ||
| links |
| ||
| inv.nr. | 404504 10.1.145.13 AIM 2601000564"404504 10.1.145.13 <br /> AIM 2601000564" cannot be used as a page name in this wiki. |
