W1 - Olympus TIRF
From BioDIP
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| description = Olympus TIRF setup. | | description = Olympus TIRF setup. | ||
| applications = membrane trafficking studies | | applications = membrane trafficking studies | ||
| − | | microscope = manual IX71 | + | | microscope = manual IX71, manual XY stage |
| objectives = PlanApo 100x / 1.45 Oil TIRFM | | objectives = PlanApo 100x / 1.45 Oil TIRFM | ||
| illumination = HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt | | illumination = HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt | ||
Revision as of 16:15, 10 August 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , inverted stand, manual IX71, manual XY stage | ||
| objectives |
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| illumination |
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| detection | Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
| reflectors | Cairn optosplit II image splitter: two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | |||
| incubation | mobile stage incubator | ||
| links |
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| inv.nr. | 404504 |
[[Category:{{{type}}}]]
