W1 - Olympus TIRF
From BioDIP
(Difference between revisions)
| Line 1: | Line 1: | ||
{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=MPI06 |
| − | | system-name | + | |system-name=Olympus TIRF |
| − | | category | + | |type=Microscope |
| − | + | |category=Total Internal Reflection | |
| − | + | |facility=MPI-CBG LMF | |
| − | | facility | + | |building=MPI-CBG |
| − | + | |room=038B | |
| − | | building | + | |facility_image=logo-lmf.jpg |
| − | | room | + | |description=Olympus TIRF setup. |
| − | | description | + | |applications=membrane trafficking studies |
| − | | applications | + | |image=MPI06.jpg |
| − | | microscope | + | |stand=3D |
| − | | objectives | + | |microscope=manual IX71, manual XY stage |
| − | | illumination | + | |objectives=PlanApo 100x / 1.45 Oil TIRFM |
| − | | detection | + | |illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt |
| + | |detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um | ||
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
| − | | reflectors | + | |reflectors=Cairn optosplit II image splitter:<br>two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em<br>Sutter filter wheel: <br>position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73<br> microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 |
| − | | features | + | |features=custom-built TIRF condenser, manual TIRF angle adjustment |
| − | | incubation | + | |incubation=stage incubator (T+CO2) + objective heater |
| − | | link1 | + | |link1=[http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA laser power] |
| − | | link2 | + | |link2=- |
| − | | inv | + | |inv=404504 |
| + | |obj1=Empty position | ||
| + | |obj2=Empty position | ||
| + | |obj3=Empty position | ||
| + | |obj4=Empty position | ||
| + | |obj5=Empty position | ||
| + | |obj6=Empty position | ||
| + | |obj7=Empty position | ||
| + | |obj8=Empty position | ||
}} | }} | ||
Revision as of 16:57, 1 September 2009
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , 3D stand, manual IX71, manual XY stage | ||
| objectives | |||
| illumination |
| ||
| detection | Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
| reflectors | Cairn optosplit II image splitter: two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | |||
| incubation | stage incubator (T+CO2) + objective heater | ||
| links |
| ||
| inv.nr. | 404504 |
