W1 - Olympus TIRF
From BioDIP
(Difference between revisions)
| Line 21: | Line 21: | ||
|obj7=- | |obj7=- | ||
|obj8=- | |obj8=- | ||
| − | | | + | |ill1=Transmitted Light (Halogen) |
| + | |ill2=Fluorescence (HBO) | ||
| + | |ill3=Laser DPSS 491 nm | ||
| + | |ill4=Laser DPSS 561 nm | ||
| + | |ill5=- | ||
| + | |ill6=- | ||
| + | |ill7=- | ||
| + | |ill8=- | ||
|detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um | |detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um | ||
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
| Line 31: | Line 38: | ||
|inv=404504 | |inv=404504 | ||
|objectives=PlanApo 100x / 1.45 Oil TIRFM | |objectives=PlanApo 100x / 1.45 Oil TIRFM | ||
| + | |illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt | ||
}} | }} | ||
Revision as of 07:44, 15 September 2009
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , Inverted stand, manual IX71, manual XY stage | ||
| objectives | |||
| illumination | |||
| detection | Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
| reflectors | Cairn optosplit II image splitter: two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | |||
| incubation | stage incubator (T+CO2) + objective heater | ||
| links |
| ||
| inv.nr. | 404504 |
