W1 - Olympus TIRF
From BioDIP
(Difference between revisions)
(640 laser + W-View info added) |
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|ill3=Laser DPSS 491 nm | |ill3=Laser DPSS 491 nm | ||
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
| + | |ill5=Laser DPSS 640 nm | ||
|detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um | |detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um | ||
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
| − | |reflectors= | + | |reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40<br>Sutter filter wheel: <br>position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73<br> microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 |
|features=custom-built TIRF condenser, manual TIRF angle adjustment | |features=custom-built TIRF condenser, manual TIRF angle adjustment | ||
|incubation=Stage incubator (T+CO2) + objective heater | |incubation=Stage incubator (T+CO2) + objective heater | ||
Revision as of 01:29, 7 November 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , Inverted stand, manual IX71, manual XY stage | ||
| objectives | |||
| illumination | |||
| detection | Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
| reflectors | Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40 Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | |||
| incubation | Stage incubator (T+CO2) + objective heater | ||
| links |
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| inv.nr. | 404504 |
