W1 - Olympus TIRF
From BioDIP
(Difference between revisions)
(added 150x calibration) |
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|building=MPI-CBG | |building=MPI-CBG | ||
|room=340 | |room=340 | ||
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|description=Olympus TIRF setup. | |description=Olympus TIRF setup. | ||
|applications=membrane trafficking studies | |applications=membrane trafficking studies | ||
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|detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um | |detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um | ||
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
| + | with 150x objective: 0.106um (measured) | ||
|reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40<br>Sutter filter wheel: <br>position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: EdgeBasic 635 LP, 8: BL HC LP488, 9: BL HC 617/73<br> microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | |reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40<br>Sutter filter wheel: <br>position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: EdgeBasic 635 LP, 8: BL HC LP488, 9: BL HC 617/73<br> microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
|features=custom-built TIRF condenser, manual TIRF angle adjustment | |features=custom-built TIRF condenser, manual TIRF angle adjustment | ||
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|link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA | |link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA | ||
|inv=404504 | |inv=404504 | ||
| + | |facility_image=logo-lmf.jpg | ||
|objectives=PlanApo 100x / 1.45 Oil TIRFM | |objectives=PlanApo 100x / 1.45 Oil TIRFM | ||
|illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt | |illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt | ||
}} | }} | ||
Revision as of 16:20, 6 January 2011
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Olympus - IX71, Inverted stand, manual, manual XY stage | ||
| objectives | |||
| illumination | |||
| detection | Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; with 150x objective: 0.106um (measured) | ||
| reflectors | Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40 Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: EdgeBasic 635 LP, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | iQ 1.9.1 | ||
| incubation | Stage incubator (T+CO2) + objective heater | ||
| links |
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| inv.nr. | 404504 |
