W1 - Olympus TIRF
From BioDIP
(Difference between revisions)
(update after relocation: removed sutter wheel, new iXon Ultra camera info, new iQ version; added possible applications) |
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|room=U32 | |room=U32 | ||
|description=Olympus TIRF setup. | |description=Olympus TIRF setup. | ||
| − | |applications=membrane trafficking studies | + | |applications=membrane trafficking studies; single molecule imaging; life yeast experiments |
|image=MPI_W1_TIRF.jpg | |image=MPI_W1_TIRF.jpg | ||
|stand-name=Olympus - IX71 | |stand-name=Olympus - IX71 | ||
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|ill3=Laser DPSS 491 nm | |ill3=Laser DPSS 491 nm | ||
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
| − | |detection=Andor iXon | + | |detection=Andor iXon Ultra 897 BV back illuminated EMCCD [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:Andor_iXon_Ultra_897_Specifications.pdf spec sheet]; pixel size of EMCCD chip: 16um; |
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
with 150x objective: 0.106um (measured) | with 150x objective: 0.106um (measured) | ||
| − | |reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40 | + | |reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40<br>microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 |
|features=custom-built TIRF condenser, manual TIRF angle adjustment | |features=custom-built TIRF condenser, manual TIRF angle adjustment | ||
| − | |software=iQ | + | |software=iQ 2.6 |
|incubation=Stage incubator (T+CO2) + objective heater | |incubation=Stage incubator (T+CO2) + objective heater | ||
|link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA | |link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA | ||
Revision as of 10:02, 29 June 2012
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Olympus - IX71, Inverted stand, manual, manual XY stage | ||
| objectives | |||
| illumination | |||
| detection | [[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet; pixel size of EMCCD chip: 16um;
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; with 150x objective: 0.106um (measured)]] | ||
| reflectors | Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | iQ 2.6 | ||
| incubation | Stage incubator (T+CO2) + objective heater | ||
| links |
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| inv.nr. | 404504 10.1.145.13 AIM 2601000564"404504 10.1.145.13 <br /> AIM 2601000564" cannot be used as a page name in this wiki. |
