W1 - Olympus TIRF
From BioDIP
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|building=MPI-CBG | |building=MPI-CBG | ||
|room=U32 | |room=U32 | ||
+ | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
+ | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=Olympus TIRF setup. | |description=Olympus TIRF setup. | ||
− | |applications=membrane trafficking studies; single molecule imaging; life yeast experiments | + | |applications=membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics |
|image=MPI_W1_TIRF.jpg | |image=MPI_W1_TIRF.jpg | ||
|stand-name=Olympus - IX71 | |stand-name=Olympus - IX71 | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=manual, manual XY stage | |microscope=manual, manual XY stage | ||
− | |obj1=Olympus | + | |obj1=Olympus UPlanSApochromat 10x 0.4 |
− | |obj2=Olympus | + | |obj2=Olympus MPlanFluarN 100x 0.9 |
− | | | + | |obj3=Olympus PlanApochromat 100x 1.45 Oil TIRFM |
+ | |obj4=Olympus UApochromat 150x 1.45 Oil TIRFM | ||
+ | |obj5=Olympus Apo N 100x 1.70 HIOil TIRFM | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (Metal Halide, X-Cite, 120W) | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) | ||
|ill3=Laser DPSS 491 nm | |ill3=Laser DPSS 491 nm | ||
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
− | |detection=Andor iXon Ultra 897 BV back illuminated EMCCD [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:Andor_iXon_Ultra_897_Specifications.pdf spec sheet]; pixel size of EMCCD chip: 16um; | + | |detection=Andor iXon Ultra 897 BV back illuminated EMCCD [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:Andor_iXon_Ultra_897_Specifications.pdf spec sheet];<br> pixel size of EMCCD chip: 16um; |
− | image pixel size: with | + | image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar); |
− | with 150x objective: 0. | + | with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar) |
− | |reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; | + | |reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF<br>microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 |
|features=custom-built TIRF condenser, manual TIRF angle adjustment | |features=custom-built TIRF condenser, manual TIRF angle adjustment | ||
− | |software=iQ 2. | + | |software=iQ 2.9 + iQ 3.2 [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>with Python IDE |
|incubation=Stage incubator (T+CO2) + objective heater | |incubation=Stage incubator (T+CO2) + objective heater | ||
− | |link1= | + | |link1=https://docs.google.com/spreadsheets/d/1iG4mhQX-ejdwQwYcXd7k2OapcL1n7seb5BWR02L6bwg/edit?usp=sharing |
|inv=404504 10.1.145.13 <br> AIM 2601000564 | |inv=404504 10.1.145.13 <br> AIM 2601000564 | ||
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}} | }} |
Latest revision as of 18:22, 10 December 2018
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
microscope | Olympus - IX71, Inverted stand, manual, manual XY stage | ||
objectives | |||
illumination | |||
detection | [[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet; pixel size of EMCCD chip: 16um; image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar); with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar)]] | ||
reflectors | Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
software | [[software::iQ 2.9 + iQ 3.2 iQ3 user guide with Python IDE]] | ||
incubation | Stage incubator (T+CO2) + objective heater | ||
links |
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inv.nr. | 404504 10.1.145.13 AIM 2601000564"404504 10.1.145.13 <br /> AIM 2601000564" cannot be used as a page name in this wiki. |