W1 - Olympus TIRF
From BioDIP
(Difference between revisions)
Line 3: | Line 3: | ||
| system-name = Olympus TIRF | | system-name = Olympus TIRF | ||
| category = Total Internal Reflection | | category = Total Internal Reflection | ||
− | | stand = | + | | stand = IX71, manual |
| image = MPI06.jpg | | image = MPI06.jpg | ||
| facility = MPI-CBG LMF | | facility = MPI-CBG LMF | ||
Line 12: | Line 12: | ||
| applications = membrane trafficking studies | | applications = membrane trafficking studies | ||
| microscope = inverted stand, manual XY stage | | microscope = inverted stand, manual XY stage | ||
− | | objectives = | + | | objectives = PlanApo 100x / 1.45 Oil TIRFM |
− | | illumination = HBO<br>491 nm DPSS laser<br>561 nm DPSS laser | + | | illumination = HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt |
− | | detection = | + | | detection = Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um |
− | | reflectors = | + | image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; |
− | | features = | + | | reflectors = Cairn optosplit II image splitter:<br>two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em<br>Sutter filter wheel: <br>position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73<br> microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 |
+ | | features = custom-built TIRF condenser, manual TIRF angle adjustment | ||
| incubation = mobile stage incubator | | incubation = mobile stage incubator | ||
| link1 = [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA laser power] | | link1 = [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA laser power] |
Revision as of 15:03, 23 January 2009
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | , IX71, manual stand, inverted stand, manual XY stage | ||
objectives |
| ||
illumination |
| ||
detection | Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; | ||
reflectors | Cairn optosplit II image splitter: two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
software | |||
incubation | mobile stage incubator | ||
links |
| ||
inv.nr. | 404504 |
[[Category:{{{type}}}]]