W1 - Olympus TIRF

Suitable for membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics.

Directions

MPI-CBG LMF (MPI-CBG, U32)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX71, Inverted stand, manual, manual XY stage
objectives	Olympus UPlanSApochromat 10x 0.4 Olympus PlanApochromat 100x 1.45 TIRFM Olympus UApochromat 150x 1.45 Oil TIRFM Olympus NPlanFluarN 100x 0.9
illumination	Transmitted Light (Halogen) Fluorescence (Metal Halide, X-Cite, 120W) Laser DPSS 491 nm Laser DPSS 561 nm
detection	[[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet; pixel size of EMCCD chip: 16um; image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar); with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar)]]
reflectors	Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575
features	custom-built TIRF condenser, manual TIRF angle adjustment
software	[[software::iQ 2.9 + iQ 3.0 iQ3 user guide]]
incubation	Stage incubator (T+CO2) + objective heater
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.	404504 10.1.145.13 AIM 2601000564"404504 10.1.145.13 <br /> AIM 2601000564" cannot be used as a page name in this wiki.

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