W1 - Olympus TIRF

Suitable for membrane trafficking studies.

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	, inverted stand, manual IX71, inverted stand, manual XY stage
objectives
illumination
detection	Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um;
reflectors	Cairn optosplit II image splitter: two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575
features	custom-built TIRF condenser, manual TIRF angle adjustment
software
incubation	mobile stage incubator
links	[ manual] [laser power laser power values] [- beam path] [ advanced system check]
inv.nr.	404504

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